Food and Fermentation Industries >

2023 , Vol. 49 >Issue 1: 273 - 278

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030317

Determination of sialic acid in dairy products by liquid chromatography-tandem mass spectrometry with stable isotope internal standard

  • HE Shu ,
  • CAO Jing
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  • (College of Chemistry and Material Science, Shanghai Normal University, Shanghai 200234, China)

Received date: 2021-12-06

  Revised date: 2022-01-07

  Online published: 2023-02-14

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Abstract

A quantitative method with stable isotope internal standard (SIIS) for the determination of N-acetylneuraminic acid (Neu5Ac) in dairy products by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established. In this research, for the first time, SIIS coupled with LC-MS/MS to quantify Neu5Ac was employed. LC-MS/MS was done in negative ion and multi-reaction monitoring mode. This study also optimized experimental conditions including sample preparation and LC-MS conditions. Totally 6 kinds of dairy products were detected by this method. Results showed that the linear range of sialic acid was 0.0125-5 μg/mL and the correlation coefficient was 0.999 9. The recoveries of the spiked samples were between 76.5% and 96.4%, and the relative standard deviations (n=6) were between 0.6% and 6.35%. The limit of detection and limit of quantification were 0.4 ng/mL and 1.6 ng/mL, respectively. The quantitative results of the SIIS method were accurate and reliable. Thus, the method explored here can be used for the determination of sialic acid in different dairy products.

Key words： sialic acid; LC-MS/MS; stable isotope internal standard; dairy products

Cite this article

HE Shu , CAO Jing . Determination of sialic acid in dairy products by liquid chromatography-tandem mass spectrometry with stable isotope internal standard[J]. Food and Fermentation Industries, 2023 , 49(1) : 273 -278 . DOI: 10.13995/j.cnki.11-1802/ts.030317

References

[1] WANG B.Sialic acid is an essential nutrient for brain development and cognition[J].Annual Review of Nutrition, 2009, 29:177-222.
[2] 任艳娇, 刘玉淑, 伍姚, 等.超高效液相色谱-串联四级杆飞行时间质谱测定奶粉中N-乙酰神经氨酸[J].食品与发酵工业, 2019, 45(19):287-291.
REN Y J, LIU Y S, WU Y, et al.Evaluation of N-acetylneuraminic acid in milk powder by ultra-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry[J].Food and Fermentation Industries, 2019, 45(19):287-291.
[3] DU J J, ZHANG Q W, LI J J, et al.LC-MS in combination with DMBA derivatization for sialic acid speciation and distribution analysis in fish tissues[J].Analytical Methods, 2020, 12(17):2 221-2 227.
[4] SCHAUER R.Sialic acids as regulators of molecular and cellular interactions[J].Current Opinion in Structural Biology, 2009, 19(5):507-514.
[5] WANG B.Molecular mechanism underlying sialic acid as an essential nutrient for brain development and cognition[J].Advances in Nutrition, 2012, 3(3):465S-472S.
[6] WANG B, MCVEAGH P, PETOCZ P, et al.Brain ganglioside and glycoprotein sialic acid in breastfed compared with formula-fed infants[J].The American Journal of Clinical Nutrition, 2003, 78(5):1 024-1 029.
[7] ANDERSON J W, JOHNSTONE B M, REMLEY D T.Breast-feeding and cognitive development:A meta-analysis[J].The American Journal of Clinical Nutrition, 1999, 70(4):525-535.
[8] 黄华军, 奚星林, 吴宏中, 等.奶粉中唾液酸的测定方法[J].分析测试学报, 2006(4):129-131.
HUANG H J, XI X L, WU H Z, et al.Determination of sialic acid in milk powder[J].Journal of Instrumental Analysis, 2006(4):129-131.
[9] 黄华军, 奚星林, 陈文锐, 等.分光光度法检测燕窝及其制品中燕窝含量[J].广州食品工业科技, 2003, 19(3):68-69.
HUANG H J, XI X L, CHEN W R, et al.Determination of content of bird nest by spectrophotometer[J].Guangzhou Food Science and Technology, 2003, 19(3):68-69.
[10] 李敏, 黄华军, 奚星林, 等.燕窝中唾液酸含量的分光光度测定方法[J].中国卫生检验杂志, 2011, 21(3):598-600.
LI M, HUANG H J, XI X L, et al.Determination of sialic acid in bird nest by spectrophotometer[J].Chinese Journal of Health Laboratory Technology, 2011, 21(3):598-600.
[11] 王羚郦, 吴国洪, 邱子博, 等.九种燕窝中唾液酸含量的紫外可见分光光度法测定[J].时珍国医国药, 2013, 24(11):2 571-2 573.
WANG L L, WU G H, QIU Z B, et al.The determination of sialic acid in nine kinds of edible bird's nest by UV-Vis spectrometry[J].Lishizhen Medicine and Materia Medica Research, 2013, 24(11):2 571-2 573.
[12] 王蕾, 李晓东.高效液相色谱法检测婴儿配方奶粉中唾液酸[J].中国乳品工业, 2013, 41(5):52-55.
WANG L, LI X D.Deteminnation of total sialic acid content in infant formula by HPLC-FLD[J].China Dairy Industry, 2013, 41(5):52-55.
[13] 王天娇, 汤鋆, 胡争艳, 等.高效液相色谱法快速检测人血清中唾液酸[J].卫生研究, 2021, 50(2):333-336.
WANG T J, TANG J, HU Z Y, et al.Rapid determination of sialic acid in human serum by high performance liquid chromatography[J].Journal of Hygiene Research, 2021, 50(2):333-336.
[14] 徐柳柳, 陈丹, 马凡提, 等.高效液相色谱法检测酸奶中的唾液酸[J].中国食品添加剂, 2020, 31(5):107-112.
XU L L, CHEN D, MA F T, et al.Determination of sialic acid in yogurt by high performance liquid chromatography[J].China Food Additives, 2020, 31(5):107-112.
[15] TANG K T, LIANG L N, CAI Y Q, et al.Determination of sialic acid in milk and products using high performance anion-exchange chromatography coupled with pulsed amperometric detection[J].Chinese Journal of Analytical Chemistry, 2008, 36(11):1 535-1 538.
[16] LACOMBA R, SALCEDO J, ALEGRÍA A, et al.Determination of sialic acid and gangliosides in biological samples and dairy products:A review[J].Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(2):346-357.
[17] 赖心田, 张世伟, 陈血建, 等.酶联免疫法定量检测市售燕窝及其加工制品中特征蛋白含量[J].农产品加工(学刊), 2013(18):4-7;12.
LAI X T, ZHANG S W, CHEN X J, et al.Enzyme linked immunosorbent assay for sialoglycoprotein in commercially available edible bird's nest and its products[J].Academic Periodical of Farm Products Processing, 2013(18):4-7;12.
[18] SHAW C J, CHAO H, XIAO B M.Determination of sialic acids by liquid chromatography-mass spectrometry[J].Journal of Chromatography A, 2001, 913(1-2):365-370.
[19] WANG D, ZHOU X, WANG L, et al.Quantification of free sialic acid in human plasma through a robust quinoxalinone derivatization and LC-MS/MS using isotope-labeled standard calibration[J].Journal of Chromatography B, 2014, 944:75-81.
[20] 刘彦品, 刘利娟, 牟光庆, 等.超高效液相色谱-串联四极杆质谱法测定奶粉中唾液酸的含量[J].中国奶牛, 2015(17):44-48.
LIU Y P, LIU L J, MU G Q, et al.Determination of sialic acid in formula using UPLC-MS/MS[J].China Dairy Cattle, 2015(17):44-48.
[21] 茹元朴, 陈君, 张明辉, 等.高效阴离子交换色谱-脉冲安培法测定母乳及婴儿配方粉中的唾液酸[J].食品与发酵工业, 2021, 47(11):221-226.
RU Y P, CHEN J, ZHANG M H, et al.Determination of sialic acids in human milk and infant formulas by high-performance anion-exchange chromatography with pulsed amperometric detection[J].Food and Fermentation Industries, 2021, 47(11):221-226.
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