To develop selenium-enriched oyster peptides with antioxidant and angiotensin converting enzyme (ACE)inhibitory activities, selenium-enriched oyster peptides were prepared from selenium-enriched oyster protein, and its selenium content, amino acid composition, antioxidant activity, and ACE inhibitory activities were evaluated. Results showed that the optimal enzymatic conditions were as follows: Hydrolysis time of 4 h,temperature of 37 ℃, enzyme/substrate ratio of 0.5%, pH 1.0, and substrate mass concentration of 7 g/100mL. The selenium-enriched oyster peptidesprepared under these conditions were rich in hydrophobic and acidic amino acids related to antioxidant and ACE inhibitory activities, and the EC50 values for scavenging DPPH radicals and ABTS cation radicals were 1.365 and 1.074 mg/mL, respectively. Selenium-enriched oyster peptides showed good cellular antioxidant activity (EC50: 1.114 μg/mL) and had a significant protective effect on oxidative damage of HepG2 cells in a dose-effect relationship. In addition, selenium-enriched oyster peptides showed strong ACE inhibitory activity, which might be intrinsically related to its good antioxidant activity. Selenium-enriched oyster peptides have good antioxidant and antihypertensive activities, which lay a preliminary foundation for the further study of selenium-enriched peptides from oysters, and provide a theoretical basis for the development of natural selenium-enriched nutritional health food.
XIA Zhen
,
CHEN Bingbing
,
HUANG Wen
,
KANG Ao
,
LIANG Xingtang
,
YIN Yanzhen
,
TANG Dejian
,
MENG Li
,
LIU Guo
,
CAO Yong
,
MIAO Jianyin
. Preparation of selenium-enriched oyster peptides and their antioxidant and angiotensin converting enzyme inhibitory activities[J]. Food and Fermentation Industries, 2023
, 49(2)
: 120
-128
.
DOI: 10.13995/j.cnki.11-1802/ts.030065
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