Food and Fermentation Industries >

2023 , Vol. 49 >Issue 2: 226 - 233

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030567

Effects of acute temperature stress on lipid peroxidation and antioxidant physiological responses of different tissues of razor clam Sinonovacula constricta

  • FANG Jiaqi ,
  • ZHANG Min ,
  • ZHENG Kai ,
  • JIA Miao ,
  • LI Qixun ,
  • LI Yushen ,
  • WANG Hongting
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(National Experimental Teaching Demonstration Center for Food Science and Engineering(Shanghai Ocean University), Shanghai 201306, China)
    3(Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China)

Received date: 2021-12-30

  Revised date: 2022-01-12

  Online published: 2023-02-15

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Abstract

To investigate the oxidative stress and lipid peroxidation of Sinonovacula constricta under acute temperature stress, and to compare the antioxidative physiological response of different tissues of Sinonovacula constricta,the temporarily cultured Sinonovacula constrictawas divided into 5 groups. After temperature stress in a water bath at 25, 28, 30, 32, and 35 ℃, malondialdehyde (MDA) content, hydrogen peroxide(H2O2) content, superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione peroxidase (GPX) activity, glutathione (GSH) content, and total antioxidant capacity (T-AOC) were determined in the gill, digestive gland, and mantle tissue of Sinonovacula constricta. Results showed that the SOD, CAT, GPX, T-AOC activity, and GSH content of Sinonovacula constricta were the highest in the digestive gland under the temperature stress of 25-35 ℃. The SOD, T-AOC activity, and GSH content increased first and then decreased with the increase intemperature, and was the highest at 28 ℃. The CAT activity decreased with the increase intemperature, and then reached the minimum (19.87 U/mg) at 35 ℃. The trend of GPX activity was opposite to that of CAT activity, which was positively correlated with stress temperature and reached the maximum (172.36 U/mg) at 35 ℃. The level of MDA content was the highest inthe mantle, followed by gills, and the lowest inthe digestive gland, and all of them reached the maximum at 35 ℃. Compared with the mantle and gills, the digestive glands responded to oxidative stress in a timely manner, activated the antioxidant defense system and removed excess reactive oxygen species in vivo, and the overall antioxidant capacity was better.

Key words： Sinonovacula constricta; different tissues; acute temperature stress; oxidative stress; lipid peroxidation

Cite this article

FANG Jiaqi , ZHANG Min , ZHENG Kai , JIA Miao , LI Qixun , LI Yushen , WANG Hongting . Effects of acute temperature stress on lipid peroxidation and antioxidant physiological responses of different tissues of razor clam Sinonovacula constricta[J]. Food and Fermentation Industries, 2023 , 49(2) : 226 -233 . DOI: 10.13995/j.cnki.11-1802/ts.030567

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