A method for the determination of polaramycin A from Streptomyces hygroscopicus (LP-93) fermentation broth by HPLC was established in this work. C18 column (250 mm×4.6 mm, 5 μm) and acetonitrile-water (45∶55) mobile phase were employed, as well the related parameters: flow rate of 1.0 mL/min, detection wavelength of 220 nm, injection volume of 10 μL, and column temperature of 30 ℃. On this basis, a high performance preparative chromatography (HPPC) purification method of polaramycin A was also established, in which C18 column (250 mm×21.2 mm, 7 μm), a flow rate of 15 mL/min and injection volume of 3 mL were employed. Further, the purified fraction was qualitatively analyzed by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometer (UPLC-Q-TOF). The results showed that the method exhibited good linearity with a coefficient of determination (R2=0.999 8) in the range of 2.5-500 mg/L. The average recoveries of the analyte were between 90.53%-101.96%, with relative standard deviation (RSD) less than 2%. Polaramycin A solution with high purity was obtained, with an average purity of 87.52% and an average yield of 71.84%. MS1 and MS2 spectra of polaramycin A were acquired. The method is rapid, simple, and accurate, which can be used for the determination of polaramycin A from S. hygroscopicus fermentation broth, and it provides a reference for the purification of this natural product.
SUN Bo
,
MA Sitong
,
SONG Jia
,
WANG Hui
,
MIAO Haijiang
,
YANG Zhaoyong
,
ZHAO Chen
. Determination and purification of polaramycin A from Streptomyces hygroscopicus fermentation broth by HPLC[J]. Food and Fermentation Industries, 2023
, 49(2)
: 246
-250
.
DOI: 10.13995/j.cnki.11-1802/ts.031125
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