Food and Fermentation Industries >

2023 , Vol. 49 >Issue 3: 9 - 15

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031795

Molecular mechanism of antimicrobial lipopeptide produced by marine Bacillus amyloliquefaciens CMT-9 analyzed by LC-MS/MS and comparative genomics

  • HUA Meifang ,
  • LI Dongxun ,
  • DENG Qi ,
  • SUN Lijun ,
  • ZHONG Saiyi ,
  • LIAO Jianmeng
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Province Engineering Laboratory for Marine Biological Products, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034,China)
    3(Zhanjiang Institute of Food and Drug Control, Zhanjiang 524022, China)

Received date: 2022-04-01

  Revised date: 2022-04-28

  Online published: 2023-03-03

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Abstract

The article aims to explore the molecular mechanism of antimicrobial lipopeptide by marine Bacillus amyloliquefaciens CMT-9. LC-MS/MS was used to detect the main components of CMT-9 fermentation broth, and whole genome information was analyzed by combining Illumina high-throughput sequencing, which were in comparison with the metabolic pathways and key genes related to lipopeptide synthesis in Bacillus velezensis CMT-6, Bacillus amyloliquefaciens DSM7T, Bacillus velezensis FZB42 and Bacillus subtilis 168. The results indicated that the primary components of fermentation broth of CMT-9 were Surfactin, Iturin and Fengycin. From the whole genome sequencing analysis, the genome length of the CMT-9 strain chromosome is 3 928 149 bp, with 43.39% G+C, 83 tRNA and 9 rRNA. From GO, KEGG and COG databases, the differential genes mainly originated from the pathways associated with lipopeptide synthesis, such as carbohydrate metabolism and amino acid metabolism. High concordances were exhibited in genomic composition, content of G+C, colinearity, homology and shared genes between CMT-9, CMT-6 and FZB42 with similar lipopeptide spectrum. The differences of lipopeptide spectrum and the molecular mechanism in B. amyloliquefaciens were analyzed simultaneously via LC-MS/MS and comparative genomics, providing a theoretical basis for the screening of high-yielding lipopeptide strains and the construction of gene library.

Key words： Bacillus amyloliquefaciens CMT-9; antimicrobial lipopeptide; high performance liquid chromatography tandem mass spectrometry (LC-MS/MS); whole genome sequencing; comparative genomics

Cite this article

HUA Meifang , LI Dongxun , DENG Qi , SUN Lijun , ZHONG Saiyi , LIAO Jianmeng . Molecular mechanism of antimicrobial lipopeptide produced by marine Bacillus amyloliquefaciens CMT-9 analyzed by LC-MS/MS and comparative genomics[J]. Food and Fermentation Industries, 2023 , 49(3) : 9 -15 . DOI: 10.13995/j.cnki.11-1802/ts.031795

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