The dehulled gardenia fruit was used as raw materials, X-5 macroporous resin was used to separate and prepare gardenia yellow pigment. The color value and antioxidant activities of gardenia yellow pigment before and after purification were determined. The effects of temperature, light, metal ions, and pH value on the stability of gardenia yellow pigment before and after purification were also studied. Results showed that the color value of gardenia yellow pigment increased 4.07 times from 48.51 to 243.43 when the loading mass concentration was 2.00 mg/mL, the loading flow rate was 1.50 mL/min, the ethanol volume fraction was 80%, and the elution flow rate was 3.00 mL/min. The DPPH radical scavenging capacity, ferric-reducing antioxidant power, and ABTS cationic radical scavenging capacity were increased by 12.77, 6.73, and 21.66 times, respectively. In addition, compared with the gardenia yellow pigment before purification, the gardenia yellow pigment had higher stability after purification. The gardenia yellow pigment before and after purification showed poor stability at high temperatures, ultraviolet light, Fe3+, strong acids, and strong alkalis. This article aims to provide a theoretical basis for the development and application of gardenia yellow pigment, and it is of great significance to the research and development of healthy foods.
TANG Liqin
,
XU Yujuan
,
WU Jijun
,
LIU Haocheng
,
YU Yuanshan
,
LIN Xian
,
FU Manqin
,
PENG Jian
,
WEN Jing
. Purification, physicochemical properties, and stability evaluation of gardenia yellow pigment[J]. Food and Fermentation Industries, 2023
, 49(3)
: 189
-196
.
DOI: 10.13995/j.cnki.11-1802/ts.030125
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