Food and Fermentation Industries >

2023 , Vol. 49 >Issue 3: 306 - 313

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032719

Establishment and evaluation of LC-MS/MS method for ten mycotoxins in Xiangpai brine

  • AI Daoying ,
  • ZHENG Yirou ,
  • HUANG Zhanrui ,
  • ZHAO Liangzhong ,
  • LIN Lidan ,
  • ZHOU Hengping ,
  • ZHOU Kai ,
  • ZHOU Jinsong ,
  • YIN Shixian
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  • 1(College of Food and Chemical Engineering, Shaoyang University, Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang 422000, China)
    2(Department of Industry-University-Research Collaboration, Shenzhen Total-Test Technology Co.Ltd., Guangdong Engineering Research Center of High Specificity Biological Rapid Detection, Shenzhen 518038, China)
    3(Pingjiang Jinzai Food Co.Ltd., Yueyang 414000, China)

Received date: 2022-06-22

  Revised date: 2022-07-20

  Online published: 2023-03-03

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Abstract

This study established a method for the determination of ten mycotoxins in Xiangpai brine, and carried out methodological validation and actual sample detection based on high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS). The sample (added with isotope internal standard synchronously) was extracted by ultrasound and centrifugation with V(acetonitrile)∶V(water)∶V(acetic acid) =84∶15∶1, and the supernatant was diluted and purified with water. Methanol-water solution was used as mobile phase gradient elution, and electrospray positive and negative multi-reaction ion monitoring mode was adopted. Qualitative and quantitative analysis was carried out by isotope internal standard quantitative method. The results showed that the linearity of the ten mycotoxins were good in the range of 10-200 μg/L (R2>0.998 5), and the limits of detection and quantification were 0.1-13.0 μg/kg and 0.3-40.0 μg/kg, respectively. The spiked recoveries of the ten mycotoxins at the three concentration levels were 78.6%-107.3%, and the relative standard deviations were 2.2%-14.0%, which met the requirements of methodology. The method is easy to operate, and the recovery rate, sensitivity and detection limit all meet the methodological requirements. Thus, this method is suitable for the determination of ten mycotoxins in brine.

Key words： brine; mycotoxin; high performance liquid chromatography tandem mass spectrometry; isotope internal standard; methodological verification

Cite this article

AI Daoying , ZHENG Yirou , HUANG Zhanrui , ZHAO Liangzhong , LIN Lidan , ZHOU Hengping , ZHOU Kai , ZHOU Jinsong , YIN Shixian . Establishment and evaluation of LC-MS/MS method for ten mycotoxins in Xiangpai brine[J]. Food and Fermentation Industries, 2023 , 49(3) : 306 -313 . DOI: 10.13995/j.cnki.11-1802/ts.032719

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