In order to obtain special yeast strains for greengage wine brewing, ten strains of yeasts were isolated from sugared greengage juice and its peels, six strains with excellent fermentation performance were obtained through four stages of screening including gas production, ethanol production, aroma production and fermentation capacity, which were named as J3, J6, J7, J8, J9, and J10. 26S rDNA sequencing results showed that strains J7, J8, and J9 were Saccharomyces cerevisiae while strains J3, J6, and J10 were Hanseniaspora uvarum. The results of tolerance test exhibited that the six strains displayed good tolerances to ethanol, glucose and low pH. They all could grow at 4% ethanol, 400 g/L glucose and pH 3.1. In addition, strains J7 and J8 could tolerate 10% ethanol, J3, J6, and J10 could tolerate 450 g/L glucose, J3, J6, J7, J8, and J9 could tolerate pH 2.9. All six strains of yeasts could grow under the condition of high sugar and low pH, therefore they can meet the requirement for brewing greengage wine and are hopeful to be special fermentation yeasts with excellent characteristics for greengage wine after being domesticated.
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