Food and Fermentation Industries >

2023 , Vol. 49 >Issue 4: 250 - 257

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031964

The prevalence, virulence, and antibiotic resistance of Vibrio parahemolyticus in aquatic products and their breeding environment in Shanghai

  • LIU Junjun ,
  • LIU Danyang ,
  • LI Darong ,
  • WANG Hui ,
  • ZHAO Yong ,
  • SUN Xiaohong
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  • 1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)
    2(Laboratory of Quality and Safety Risk Assessment for Aquatic Products Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China)
    3(Shanghai Engineering Research Center for Aquatic-Products Processing & Preservation, Shanghai 201306, China)

Received date: 2022-04-13

  Revised date: 2022-05-24

  Online published: 2023-03-20

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Abstract

To investigate the prevalence, virulence, serotype, antibiotic resistance and genetic relationship of Vibrio parahaemolyticus in the aquaculture products in Shanghai, Penaeus vannamei, Ctenopharyngodon idellus, Carassius auratus and Eriocheir sinensis, as well as their aquaculture water and sediment, were collected from different farms in Shanghai inform 2020 to 2021. V. parahaemolyticus was isolated according to the National Standard GB 4789.7—2013 and PCR was used to detect thermolabile hemolysin gene (tlh), heat-resistant direct hemolysin gene (tdh), heat-resistant-related hemolysin gene (trh), Photorhabdus insect-related toxin gene (pirA) that associated with acute hepatopancreas necrosis. Multiplex PCR was used to detect serotypes. The micro-broth dilution and Enterobacter repeat intergenic consensus sequence PCR (ERIC-PCR) were used to detect antimicrobial susceptibility and genetic relationship, respectively. V. parahaemolyticus was detected in 72 of the total 234 samples (30.8%) and the pollution of P. vannamei was the most serious (78.4%). The seasonal temperature significantly affected the abundance of V. parahaemolyticus, and the pollution rate was 51.4% in summer. None of the isolates possessed pathogenic genes tdh, trh and pirA. The distribution of serotypes was diverse and the most prevalent serotype was O1 (66.7%). Seventy-two V. parahaemolyticus strains were all resistant to ampicillin and sulfamethoxazole, more than 70% strains were resistant to cefazolin and erythromycin, and 18.1% strains were resistant to sulfamethoxazole-trimoxazole. The rate of intermediate-resistance or resistance to imipenem, cefotaxime, cefepime, chloramphenicol and kanamycin was less than 5%. ERIC-PCR clusters were dispersed and showed high genetic diversity. The results of this study provide data support for the quality and safety of aquatic products in Shanghai.

Key words： Vibrio parahaemolyticus; aquaculture; serotype; antibiotic resistance; genetic diversity

Cite this article

LIU Junjun , LIU Danyang , LI Darong , WANG Hui , ZHAO Yong , SUN Xiaohong . The prevalence, virulence, and antibiotic resistance of Vibrio parahemolyticus in aquatic products and their breeding environment in Shanghai[J]. Food and Fermentation Industries, 2023 , 49(4) : 250 -257 . DOI: 10.13995/j.cnki.11-1802/ts.031964

References

[1] RYU A R, MOK J S, LEE D E, et al.Occurrence, virulence, and antimicrobial resistance of Vibrio parahaemolyticus isolated from bivalve shellfish farms along the southern coast of Korea[J].Environmental Science and Pollution Research, 2019, 26(20):21 034-21 043.
[2] JEAMSRIPONG S, KHANT W, CHUANCHUEN R.Distribution of phenotypic and genotypic antimicrobial resistance and virulence genes in Vibrio parahaemolyticus isolated from cultivated oysters and estuarine water[J].FEMS Microbiology Ecology, 2020, 96(8):fiaa081.
[3] Food and Agriculture Organization of the United Nations (FAO). Fishery and Aquaculture Statistics[EB/OL].[2022-04-07].https://www.fao.org/fishery/static/Yearbook/YB2019_USBcard/navigation/index_intro_e.htm.
[4] MOK J S, RYU A, KWON J Y, et al.Distribution of Vibrio species isolated from bivalves and bivalve culture environments along the Gyeongnam coast in Korea:Virulence and antimicrobial resistance of Vibrio parahaemolyticus isolates[J].Food Control, 2019, 106:106697.
[5] JIANG Y H, CHU Y B, XIE G S, et al.Antimicrobial resistance, virulence and genetic relationship of Vibrio parahaemolyticus in seafood from coasts of Bohai Sea and Yellow Sea, China[J].International Journal of Food Microbiology, 2019, 290:116-124.
[6] JIANG H, YU T, YANG Y T, et al. Co-occurrence of antibiotic and heavy metal resistance and sequence type diversity of Vibrio parahaemolyticus isolated from Penaeus vannamei at freshwater farms, seawater farms, and markets in Zhejiang province, China[J].Frontiers in Microbiology, 2020, 11:1294.
[7] ZAHER H A, NOFAL M I, HENDAM B M, et al.Prevalence and antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the flesh of Nile tilapia, with special reference to their virulence genes detected using multiplex PCR technique[J].Antibiotics(Basel,Switzerland), 2021, 10(6):654.
[8] YAN L, PEI X Y, ZHANG X L, et al.Occurrence of four pathogenic Vibrios in Chinese freshwater fish farms in 2016[J].Food Control, 2019, 95:85-89.
[9] BEJ A K, PATTERSON D P, BRASHER C W, et al.Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh[J].Journal of Microbiological Methods, 1999, 36(3):215-225.
[10] NARAYANAN S V, JOSEPH T C, PEERALIL S, et al.Tropical shrimp aquaculture farms harbour pathogenic Vibrio parahaemolyticus with high genetic diversity and Carbapenam resistance[J].Marine Pollution Bulletin, 2020, 160:111551.
[11] 郭书林, 陈信忠, 尤颖哲, 等.福建省南美白对虾幼体急性肝胰腺坏死病病原分离与PCR检测[J].中国动物检疫, 2019, 36(6):96-99.
GUO S L, CHEN X Z, YOU Y Z, et al.Pathogen isolation and PCR detection of AHPND in Penaeus vannamei larvae in Fujian Province[J].China Animal Health Inspection, 2019, 36(6):96-99.
[12] KRUMPERMAN P H.Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods[J].Applied and Environmental Microbiology, 1983, 46(1):165-170.
[13] 谢腾飞. 中国食源性副溶血性弧菌遗传多样性分析与冷胁迫研究[D].广州:华南理工大学, 2018.
XIE T F.Study on genetic diversity and cold stress of foodborne Vibrio parahaemolyticus in China[D].Guangzhou:South China University, 2018.
[14] 洪锦春, 钟凌, 陈惠龙, 等.福建省2017—2018年淡水鱼养殖场嗜盐性弧菌污染状况分析[J].海峡预防医学杂志, 2019, 25(6):53-54.
HONG J C, ZHONG L, CHEN H L, et al. Analysis of pollution status of halophilic Vibrio in freshwater fish farms in Fujian Province from 2017 to 2018[J]. Strait Journal of Preventive Medicine, 2019, 25(6):53-54.
[15] 秦思, 乔昕, 沈赟, 等.2016年江苏省淡水养殖环节常见致病性弧菌污染状况调查[J].食品安全质量检测学报, 2019, 10(12):3 946-3 951.
QIN S, QIAO X, SHEN Y, et al.Contamination investigation of common pathogenic Vibrio species in freshwater aquaculture processing in Jiangsu Province in 2016[J].Journal of Food Safety & Quality, 2019, 10(12):3 946-3 951.
[16] HE Y, WANG S, ZHANG J P, et al.Integrative and conjugative elements-positive Vibrio parahaemolyticus isolated from aquaculture shrimp in Jiangsu, China[J].Frontiers in Microbiology, 2019, 10:1574.
[17] RAHMAN M S, CARRARO R, CARDAZZO B, et al.Molecular typing of Vibrio parahaemolyticus strains isolated from mollusks in the North Adriatic sea[J].Foodborne Pathogens and Disease, 2017, 14(8):454-464.
[18] SILVA I P, DE SOUZA CARNEIRO C, ALICE FONTES SARAIVA M, et al.Antimicrobial resistance and potential virulence of Vibrio parahaemolyticus isolated from water and bivalve mollusks from Bahia, Brazil[J].Marine Pollution Bulletin, 2018, 131:757-762.
[19] RODGERS C, PARVEEN S, CHIGBU P, et al.Prevalence of Vibrio parahaemolyticus, and Vibrio vulnificus in blue crabs (Callinectes sapidus), seawater and sediments of the Maryland Coastal Bays[J].Journal of Applied Microbiology, 2014, 117(4):1 198-1 209.
[20] 李艳, 张晓蕾, 李金平, 等.2013—2017年感染性腹泻的病原体特点和流行特征[J].中华医院感染学杂志, 2019, 29(11):1 732-1 736.
LI Y, ZHANG X L, LI J P, et al.Etiological and epidemiological characteristics of with infectious diarrhea in a hospital from 2013 to 2017[J].Chinese Journal of Nosocomiology, 2019, 29(11):1 732-1 736.
[21] 李平, 黄涵, 钟汶兵.海口市市售贝类海产品副溶血性弧菌污染调查及耐药和毒力基因研究[J].中国食品卫生杂志, 2019, 31(4):366-370.
LI P, HUANG H, ZHONG W B.Investigation of Vibrio parahaemolyticus contamination, drug resistance and virulence genes in shellfish products sold in Haikou[J].Chinese Journal of Food Hygiene, 2019, 31(4):366-370.
[22] CHEN X, ZHU Q Y, YU F, et al.Serology, virulence and molecular characteristics of Vibrio parahaemolyticus isolated from seafood in Zhejiang Province[J].PLoS One, 2018, 13(10):e0204892.
[23] 严伟, 沈月华, 徐德顺.2019年浙江省湖州市副溶血性弧菌病原学特征分析[J].中国食品卫生杂志, 2021, 33(1):74-78.
YAN W, SHEN Y H, XU D S.Etiologic characteristics of Vibrio parahaemolyticus stains in Huzhou of Zhejiang in 2019[J].Chinese Journal of Food Hygiene, 2021, 33(1):74-78.
[24] LALITHA M K, WALTER N M, JESUDASON M, et al.An outbreak of gastroenteritis due to Vibrio parahaemolyticus in Vellore[J].The Indian Journal of Medical Research, 1983, 78:611-615.
[25] 魏文娟. 养殖源弧菌耐药性及qnrVC基因介导喹诺酮药物耐药机制研究[D].上海:上海海洋大学,2020.
WEI W J.Antimicrobial resistance and molecular mechanism of qnrVC on fluoroquinolones resistance in Vibrio spp.isolated from mariculture[D].Shanghai:Shanghai Ocean University, 2020.
[26] 强鑫华, 蔡云祥.食源性腹泻患者分离副溶血性弧菌毒力基因及药敏分析[J].中国卫生检验杂志, 2021, 31(2):155-158.
QIANG X H, CAI Y X.Virulence and antimicrobial susceptibility of Vibrio parahaemolyticus in patients with foodborne diarrhea[J].Chinese Journal of Health Laboratory Technology, 2021, 31(2):155-158.
[27] XIE T F, WU Q P, ZHANG J M, et al.Comparison of Vibrio parahaemolyticus isolates from aquatic products and clinical by antibiotic susceptibility, virulence, and molecular characterisation[J].Food Control, 2017, 71:315-321.
[28] SU C L, CHEN L M.Virulence, resistance, and genetic diversity of Vibrio parahaemolyticus recovered from commonly consumed aquatic products in Shanghai, China[J].Marine Pollution Bulletin, 2020, 160:111554.
[29] XIE T F, YU Q, TANG X, et al.Prevalence, antibiotic susceptibility and characterization of Vibrio parahaemolyticus isolates in China[J].FEMS Microbiology Letters, 2020, 367(16):fnaa136.
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