Food and Fermentation Industries >

2023 , Vol. 49 >Issue 6: 143 - 148

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032471

Enhancement of dipeptides AQ, SQ, and IQ on the resistance of GES-1 cells to alcohol damage

  • LI Mingliang ,
  • XU Jinzhen ,
  • FENG Zhiyuan ,
  • LI Yiguang ,
  • ZHANG Zhuoran ,
  • LIU Dan ,
  • FANG Lei ,
  • LIAO Qun ,
  • LIU Wenying ,
  • LIU Wenjun
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  • 1(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Engineering Research Center of Protein and Functional Peptides, Beijing 100015,China)
    2(Jiangzhong Pharmaceutical Co.Ltd., Nanchang 330096, China)

Received date: 2022-05-27

  Revised date: 2022-06-22

  Online published: 2023-04-14

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Abstract

Some food-derived dipeptides have good absorption properties as well as physiological activities, which have powerful application prospects in the development of functional foods. To elucidate the role of dipeptides AQ, SQ, and IQ in enhancing the resistance of GES-1 cells to alcohol damage. Firstly, GES-1 cells were pretreated with dipeptide for 24 h, after which a cell injury model was established using alcohol; whether the model was established by detecting cell survival rate and lactate dehydrogenase release from cells, and the ameliorative effect of dipeptide on cellular oxidative stress was evaluated by detecting intracellular ROS content, antioxidant enzyme activity and oxidative damage marker content. The repair effect of dipeptides on cellular tight junctions was evaluated by detecting the protein expression levels of intracellular tight junction proteins as well as related damage markers. The results showed that the dipeptide could reduce the oxidative stress condition of cells and the expression of damage markers, thus increase the expression level of tight junction proteins. This study demonstrated that the above-mentioned dipeptides can enhance the ability of GES-1 cells to resist alcohol damage, and have certain application value and potential in repairing alcoholic gastric mucosal damage and protecting gastric mucosal barrier function.

Key words： dipeptide; GES-1 cell; alcohol damage; tight junction protein; gastric mucosal barrier

Cite this article

LI Mingliang , XU Jinzhen , FENG Zhiyuan , LI Yiguang , ZHANG Zhuoran , LIU Dan , FANG Lei , LIAO Qun , LIU Wenying , LIU Wenjun . Enhancement of dipeptides AQ, SQ, and IQ on the resistance of GES-1 cells to alcohol damage[J]. Food and Fermentation Industries, 2023 , 49(6) : 143 -148 . DOI: 10.13995/j.cnki.11-1802/ts.032471

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