Determination of L-theanine, caffeine, tea polyphenols, and palmitic acid in green tea by gas chromatography-tandem triple quadrupole mass spectrometry

  • GAO Yaohua ,
  • DING Tao ,
  • SUN Can ,
  • LIU Bing ,
  • TENG Shuang
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  • 1(Rizhao Customs, Rizhao 276826, China)
    2(Nanjing Customs Plant and Food Testing Center, Nanjing 210019, China)
    3(College of Food Science and Technology/Supervision, Inspection and Testing Center for Quality of Meat-products (Nanjing), Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China)

Received date: 2022-04-18

  Revised date: 2022-05-17

  Online published: 2023-04-14

Abstract

A method was developed for the simultaneous determination of L-theanine, caffeine, tea polyphenols, and palmitic acid in green tea by gas chromatography-tandem triple quadrupole mass spectrometry (GC-MS/MS). The samples were immersed in water at 85 ℃ and tea samples were extracted with acetonitrile and then purified by the QuEchERs method. The analytes were separated on an HP-5MS capillary column and analyzed by GC-MS/MS. Multiple reaction monitoring (MRM) mode effectively reduced the background interference of the tea substrate. The limits of detection (LOD) of the method ranged from 0.001 to 0.003 mg/kg, the experimental results of the method validation test showed that the average recovery of these compounds was between 73% and 104%, and the relative standard deviation (RSD) was between 3.0% and 10.5%. In conclusion, it was verified that the method had good reproducibility, high accuracy, and simple operation by testing commercial tea samples, therefore, it was suitable for the determination of L-theanine, caffeine, tea polyphenols, and palmitic acid in green tea.

Cite this article

GAO Yaohua , DING Tao , SUN Can , LIU Bing , TENG Shuang . Determination of L-theanine, caffeine, tea polyphenols, and palmitic acid in green tea by gas chromatography-tandem triple quadrupole mass spectrometry[J]. Food and Fermentation Industries, 2023 , 49(6) : 276 -282 . DOI: 10.13995/j.cnki.11-1802/ts.032018

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