In this study, the aging model of mice was established by D-galactose. The effect of a newly discovered lactic acid bacteria (Lactobacillus delbrueckii subsp. bulgaricus KSFY07, LDSBKSFY07) on the improvement of exercise ability in aging mice and its mechanism were observed. The study examined the effect and mechanism of LDSBKSFY07 on improving the exercise ability of mice by observing the running ability, swimming endurance, blood biochemical indexes, histopathological changes and mRNA expression of aging mice, and the microbial community in the intestinal of mice. The results of animal experiments showed that LDSBKSFY07 (1.5×109 CFU/kg BW gavage) prolonged the running time and exhaustive swimming time of aging mice, and reduced blood urea nitrogen (BUN), blood lactic acid (BLA), malondialdehyde (MDA) in the serum of aging mice, and increased the levels of hepatic glycogen (HG), muscle glycogen (MG) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px). Histopathological observations suggested that LDSBKSFY07 alleviated the aging damage of mouse liver and kidney tissues. LDSBKSFY07 also down-regulated the expression of inducible nitric oxide synthase (iNOS), TNF-α mRNA expression and up-regulated the expression of neuronal nitric oxide synthase (nNOS), Cu/Zn-SOD, Mn-SOD, catalase (CAT) in liver, kidney, and skeletal muscle tissue of aging mice. In addition, LDSBKSFY07 down-regulated syncytin-1 mRNA expression in skeletal muscle tissue of aging mice. Meanwhile, LDSBKSFY07 increased the abundance of Firmicutes, Lactobacillus and Bifidobacterium and decreased the abundance of Bacteroides in the gut of aging mice. Moreover, LDSBKSFY07 had a better regulatory effect on the status of tissue, serum and intestinal flora than vitamin C (150 mg/kg BW by gavage), which could slow down the aging of mice and improve their exercise ability.
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