Food and Fermentation Industries >

2023 , Vol. 49 >Issue 7: 212 - 217

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031386

Effect of ethanol on extraction purity of egg yolk phospholipids and solubility of egg yolk powder

  • GAO Jie ,
  • QI Libo ,
  • LIN Songyi ,
  • BAO Zhijie
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  • (School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2022-03-03

  Revised date: 2022-04-13

  Online published: 2023-04-28

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Abstract

To improve the extraction purity of egg yolk phospholipids and explore the change mechanism of the solubility of defatted egg yolk powder by different ethanol elution times, egg yolk phospholipids were extracted from egg yolk powder by ethanol-assisted supercritical CO2 oil extraction technology. The purity changes of egg yolk powder extracted phospholipids under different elution times of entrainer ethanol was analyzed by rod thin layer chromatography, and then the solubility change mechanism of defatted egg yolk powder was studied by Fourier infrared spectroscopy (FT-IR), fluorescence spectroscopy, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Results showed that the purity of egg yolk phospholipid increased gradually with the increase of ethanol elution times (P<0.05). FT-IR showed that the lipid peak disappeared gradually. Fluorescence spectra showed that the surface hydrophobicity increased gradually. The results of SEM showed that the protein and lipid of defatted egg yolk powder eluted with ethanol for 5 times were completely separated. In conclusion, the purity of egg yolk phospholipids extracted by 5 times of ethanol elution-assisted supercritical CO2 was the highest, and the solubility of defatted egg yolk powder before and after ethanol elution was not significantly different (P>0.05).

Key words： supercritical CO2 fluid extraction; egg yolk lecithin; degreased egg yolk powder; physicochemical characterization; solubility

Cite this article

GAO Jie , QI Libo , LIN Songyi , BAO Zhijie . Effect of ethanol on extraction purity of egg yolk phospholipids and solubility of egg yolk powder[J]. Food and Fermentation Industries, 2023 , 49(7) : 212 -217 . DOI: 10.13995/j.cnki.11-1802/ts.031386

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