To improve the production of selenium polysaccharide from submerged fermentation of Antrodia cinnamomea, and to explore the changes of polysaccharide activity after selenium enrichment. Biomass, polysaccharide content, selenium content and selenium enrichment rate were used as evaluating indicator. The fermentation conditions of polysaccharide were optimized by single factor experiment and orthogonal experiment. Hence, the best fermentation conditions were obtained. Meanwhile, on the basis of establishing inflammatory model of mouse peritoneal macrophages (RAW264.7) induced by lipopolysaccharide (LPS), the anti-inflammatory activities of selenium polysaccharide and selenium-free polysaccharide of A. cinnamomea were evaluated. Through fermentation optimization, the optimum fermentation conditions were obtained as follows: Na2SeO3 2 mg/L, maltose 24 g/L, soluble starch 16 g/L, corn pulp dry powder 9 g/L, ammonium sulfate 9 g/L, liquid volume 75 mL/250 mL, initial pH 7, fermentation 9 d at 24 ℃, 150 r/min. The results of cell experiment in vitro showed that both of them could significantly reduce the expression of pro-inflammatory mediators such as nitrite oxide (NO), tumor necrosis factor (TNF-α), interleukin IL-6 and IL-1β, increase the activity of intracellular superoxide dismutase (SOD), and effectively inhibit the inflammatory reaction. Moreover, selenium polysaccharide is superior to selenium-free polysaccharide in increasing SOD activity and inhibiting IL-1β expression. In summary, while enriching selenium, selenium polysaccharide from A. cinnamomea have higher anti-inflammatory activity than selenium-free polysaccharide, which has a certain development prospect.
[1] JEITLER M, MICHALSEN A, FRINGS D, et al.Significance of medicinal mushrooms in integrative oncology:A narrative review[J].Frontiers in Pharmacology, 2020, 11:580656.
[2] ZHANG Y T, WANG Z, LI D Y, et al.A polysaccharide from Antrodia cinnamomea mycelia exerts antitumor activity through blocking of TOP1/TDP1-mediated DNA repair pathway[J].International Journal of Biological Macromolecules, 2018, 120:1 551-1 560.
[3] LU M K, LIN T Y, HU C H, et al.Characterization of a sulfated galactoglucan from Antrodia cinnamomea and its anticancer mechanism via TGFβ/FAK/Slug axis suppression[J].Carbohydrate Polymers, 2017, 167:229-239.
[4] LIU Y G, LI L Z, AN S S, et al.Antifatigue effects of Antrodia cinnamomea cultured mycelium via modulation of oxidative stress signaling in a mouse model[J].BioMed Research International, 2017, 2017:9374026.
[5] LIU Y Q, DING Y Q, YE M, et al.A novel heterogalactan from Antrodia camphorata and anti-angiogenic activity of its sulfated derivative[J].Polymers, 2017, 9(6):228.
[6] 殷娴, 邵蕾娜, 廖永红, 等.微生物富集有机硒研究进展[J].食品与发酵工业, 2021, 47(5):259-266.
YIN X, SHAO L N, LIAO Y H, et al.Research progress on organic selenium accumulation by microorganisms[J].Food and Fermentation Industries, 2021, 47(5):259-266.
[7] ZHU S Y, HU J H, LIU S, et al.Synthesis of Se-polysaccharide mediated by selenium oxychloride:Structure features and antiproliferative activity[J].Carbohydrate Polymers, 2020, 246:116545.
[8] LIU M, YAO W J S, ZHU Y F, et al.Characterization, antioxidant and antiinflammation of mycelia selenium polysaccharides from Hypsizygus marmoreus SK-03[J].Carbohydrate Polymers, 2018, 201:566-574.
[9] ZHOU N, LONG H R, WANG C H, et al.Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity[J].International Journal of Biological Macromolecules, 2020, 164:2 465-2 476.
[10] VIKNESHAN M, SARAVANAKUMAR R, MANGAIYARKARASI R, et al.Algal biomass as a source for novel oral nano-antimicrobial agent[J].Saudi Journal of Biological Sciences, 2020, 27(12):3 753-3 758.
[11] XIANG Q F, ZHANG W J, LI Q, et al.Investigation of the uptake and transport of polysaccharide from Se-enriched Grifola frondosa in Caco-2 cells model[J].International Journal of Biological Macromolecules, 2020, 158:1 330-1 341.
[12] 刘韫滔, 李林键, 李诚, 等.两种富硒黄牛肝菌伞多糖的制备、表征及其抗氧化活性[J] 食品科学, 2022,43(7):31-37.
LIU Y T, LI L J, LI C, et al.Preparation, characterization and antioxidant activity of two selenium-enriched polysaccharides from Suillellus luridus[J] Food Science, 2022,43(7):31-37.
[13] ZHANG B B, GUAN Y Y, HU P F, et al.Production of bioactive metabolites by submerged fermentation of the medicinal mushroom Antrodia cinnamomea:Recent advances and future development[J].Critical Reviews in Biotechnology, 2019, 39(4):541-554.
[14] WANG Z Q, ZHU C X, DAI A R, et al.Chemical characterization and antioxidant properties of cell wall polysaccharides from Antrodia cinnamomea mycelia[J].Food Bioscience, 2021, 41:100932.
[15] 肖咪, 刘利敏, 李鹏程, 等.冠突散囊菌发酵湖北海棠茶过程中主要成分及活性研究[J].食品科技, 2022, 47(1):79-86.
XIAO M, LIU L M, LI P C, et al.Dynamic analysis of the main active components and activity in fermentation process of Malus hupehensis by Eurotium cristatum[J].Food Science and Technology, 2022, 47(1):79-86.
[16] 韩瑨. 产糖乳酸菌的筛选、鉴定及其产糖条件的优化[J].微生物学杂志, 2021, 41(5):43-51.
HAN J.Screening, identification and polysaccharide biosynthesis condition optimization of EPS-Producing Lactic Acid Bacteria[J] Journal of Microbiology, 2021, 41(5):43-51.
[17] 刘玉洁, 董丽婷, 罗灿, 等.枯草芽孢杆菌LY-05发酵玉竹产水溶性多糖工艺优化及其抗氧化活性研究[J].食品工业科技, 2022, 43(3):212-221.
LIU Y J, DONG L T, LUO C, et al.Optimization of fermentation process for water-soluble polysaccharides and antioxidant activity of Polygonatum odoratum fermentated by Bacillus subtilis LY-05[J].Science and Technology of Food Industry, 2022, 43(3):212-221.
[18] 唐亚丽. 富硒古尼虫草菌发酵条件优化及活性成分研究[D].天津:天津科技大学,2016.
TANG Y L.Study on optimization of fermentation conditions and active ingredients of Cordyceps gunnii mycelium selenium-riched[D] Tianjin:Tianjin University of Science and Technology, 2016.
[19] 王正齐, 张薄博, 陈磊, 等.响应面法优化樟芝胞外多糖的发酵条件[J].食品工业科技, 2018, 39(11):99-107.
WANG Z Q, ZHANG B B, CHEN L, et al.Optimization of exopolysaccharide yield produced by submerged fermentation of Antrodia cinnamomea by response surface methodology[J].Science and Technology of Food Industry, 2018, 39(11):99-107.
[20] SHU C H, LUNG M Y.Effect of pH on the production and molecular weight distribution of exopolysaccharide by Antrodia camphorata in batch cultures[J].Process Biochemistry, 2004, 39(8):931-937.
[21] CHIANG C C, CHIANG B H.Processing characteristics of submerged fermentation of Antrodia cinnamomea in airlift bioreactor[J].Biochemical Engineering Journal, 2013, 73:65-71.
[22] 苗月, 任桂红, 甄东, 等.蛹虫草多糖调节小鼠巨噬细胞RAW264.7免疫活性的分子机制[J].食品科学, 2019, 40(9):188-194.
MIAO Y, REN G H, ZHEN D, et al.Molecular mechanism of Cordyceps militaris polysaccharides in regulating the immune function of macrophage RAW264.7 cells[J]. Food Science, 2019, 40(9):188-194.
[23] HUANG T T, WU S P, CHONG K Y, et al.The medicinal fungus Antrodia cinnamomea suppresses inflammation by inhibiting the NLRP3 inflammasome[J].Journal of Ethnopharmacology, 2014, 155(1):154-164.
[24] HSEU Y C, WU F Y, WU J J, et al.Anti-inflammatory potential of Antrodia camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-κB pathway[J].International Immunopharmacology, 2005, 5(13-14):1 914-1 925.
[25] LU M K, LEE M H, CHAO C H, et al.Physiochemical changes and mechanisms of anti-inflammation effect of sulfated polysaccharides from ammonium sulfate feeding of Antrodia cinnamomea[J].International Journal of Biological Macromolecules(Basel, Switerland), 2020, 148:715-721.
[26] ZHENG J P, JIAO S M, LI Q Y, et al.Antrodia cinnamomea oligosaccharides suppress lipopolysaccharide-induced inflammation through promoting O-GlcNAcylation and repressing p38/Akt phosphorylation[J].Molecules(Basel, Switzerland), 2017, 23(1):51.
