Food and Fermentation Industries >

2023 , Vol. 49 >Issue 9: 158 - 163

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031463

In vitro digestion characteristics and stability of ursolic acid-loaded chitosan nanoparticles

  • ZHAO Lujie ,
  • DUAN Xu ,
  • CAO Weiwei ,
  • REN Guangyue ,
  • LI Linlin ,
  • LIU Panpan ,
  • REN Xing ,
  • MIAO Junwei ,
  • BHESH Bhandari ,
  • CHEN Junliang
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Collaorative Innovation Center of Grain Storage Security (Henan Province), Zhengzhou 450001, China)
    3(School of Agriculture and Food Sciences, University of Queensland, Brisbane 4702, Australia)

Received date: 2022-03-08

  Revised date: 2022-04-06

  Online published: 2023-06-05

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Abstract

Ursolic acid (UA)-loaded chitosan nanoparticles were prepared by freeze drying (FD), microwave freeze drying (MFD), and spray drying (SD), and the storage stability and antioxidant activity of UA in UA-loaded chitosan nanoparticles in light, temperature, and in vitro simulated gastrointestinal digestion process were investigated. Results showed that the release rate of UA-loaded chitosan nanoparticles was less in the gastric digestion stage. During the intestinal digestion stage, the release rates of UA-loaded chitosan nanoparticles prepared by FD, MFD, and SD were 59%, 55%, and 50%, respectively. It showed that UA-loaded chitosan nanoparticles had sustained release properties. The results of the DPPH free radical scavenging activity in simulating the gastrointestinal digestion stage showed that the antioxidant activities of UA-loaded chitosan nanoparticles were higher than those of UA. UA and UA-loaded chitosan nanoparticles had high stability at 4 ℃, and the retention rate of UA was greater than 80%. Under light protection conditions, the retention rates of UA-loaded chitosan nanoparticles prepared by FD, MFD, and SD were 66%, 64%, and 60%, respectively. Among them, the UA-loaded chitosan nanoparticles prepared by FD had the highest activity of DPPH free radical scavenging at 4 ℃ and avoidance of light, at 2.32 and 2.09 mg/g, respectively. The stability and antioxidant activities of UA-loaded chitosan nanoparticles prepared by FD and MFD under the light, temperature, and simulated gastrointestinal digestion were higher than those of UA-loaded chitosan nanoparticles, and the stability of UA was significantly improved.

Key words： ursolic acid; chitosan nanoparticles; stability; antioxidant activity

Cite this article

ZHAO Lujie , DUAN Xu , CAO Weiwei , REN Guangyue , LI Linlin , LIU Panpan , REN Xing , MIAO Junwei , BHESH Bhandari , CHEN Junliang . In vitro digestion characteristics and stability of ursolic acid-loaded chitosan nanoparticles[J]. Food and Fermentation Industries, 2023 , 49(9) : 158 -163 . DOI: 10.13995/j.cnki.11-1802/ts.031463

References

[1] 周茜, 韩雪, 韩晓梅, 等.响应面试验优化乌梅熊果酸提取工艺及其对大肠杆菌的抑制作用[J].食品科学, 2016, 37(8):67-73.
ZHOU Q, HAN X, HAN X M, et al.Optimization of ursolic acid extraction from Fructus mume and evaluation of its antibacterial activity against Escherichia coli[J].Food Science, 2016, 37(8):67-73.
[2] 张梦, 何曼, 孙强, 等.基于Hedgehog信号通路的熊果酸诱导结直肠癌SW480细胞凋亡的机制研究[J].中草药, 2021, 52(8):2 365-2 373.
ZHANG M, HE M, SUN Q, et al.Mechanism study on ursolic acid induced apoptosis of colorectal cancer SW480 cells based on Hedgehog signaling pathway[J].Chinese Traditional and Herbal Drugs, 2021, 52(8):2 365-2 373.
[3] LÓPEZ-HORTAS L, PÉREZ-LARRÁN P, GONZÁLEZ-MUÑOZ M J, et al.Recent developments on the extraction and application of ursolic acid.A review[J].Food Research International, 2018, 103:130-149.
[4] 孙涛, 刘伟佳, 谢晶, 等.纳米壳聚糖的制备及其在食品保鲜应用中的研究进展[J].食品与发酵工业, 2020, 46(8):293-299.
SUN T, LIU W J, XIE J, et al.Preparation of nano-chitosan and its application in food preservation[J].Food and Fermentation Industries, 2020, 46(8):293-299.
[5] 张晓明, 朱良奎, 成蕾, 等.壳聚糖/海藻酸钠/多孔淀粉-茶树精油微胶囊制备及释放性能分析[J].分析化学, 2019, 47(6):862-868.
ZHANG X M, ZHU L K, CHENG L, et al.Preparation and release behavior analysis of chitosan/sodium alginate/porous starch-tea tree essential oil microcapsule[J].Chinese Journal of Analytical Chemistry, 2019, 47(6):862-868.
[6] 李嫄, 赵静, 余忠姝, 等.壳聚糖包覆姜黄素脂质体体外释放和药代动力学研究[J].中国药理学通报, 2018, 34(6):810-814.
LI Y, ZHAO J, YU Z S, et al.Release characteristics in vitro and pharmacokinetics of chitosan coated curcumin liposomes in rats[J].Chinese Pharmacological Bulletin, 2018, 34(6):810-814.
[7] 谭啸, 邱婷婷, 李若男, 等.壳聚糖纳米粒子的制备和在食品抑菌中的研究进展[J].食品科学, 2020, 41(23):347-353.
TAN X, QIU T T, LI R N, et al.Recent progress in the preparation and application of chitosan nanoparticles as an antimicrobial in foods[J].Food Science, 2020, 41(23):347-353.
[8] 赵路洁, 段续, 曹伟伟, 等.熊果酸壳聚糖纳米颗粒的制备工艺优化及评价[J].食品与发酵工业, 2022, 48(12):46-51.
ZHAO L J, DUAN X, CAO W W, et al.Optimization and evaluation of preparation of ursolic acid loaded chitosan nanoparticles[J].Food and Fermentation Industries, 2022, 48(12):46-51.
[9] ZHAO L J, DUAN X, CAO W W, et al.Effects of different drying methods on the characterization, dissolution rate and antioxidant activity of ursolic acid-loaded chitosan nanoparticles[J].Foods (Basel, Switzerland), 2021, 10(10):2470.
[10] 张秀玲, 李晨, 汲润, 等.不同保护剂对蓝靛果冻干粉理化和花色苷缓释特性的影响[J/OL].食品科学:1-12[2022-03-06].http://kns.cnki.net/kcms/detail/11.2206.TS.20210816.1510.078.html.
ZHANG X L, LI C, JI R, et al.Effects of different protective agents on the physicochemical and anthocyanin sustained-release properties of freeze-dried powder of Lonicera edulis[J].Food Science, 1-12[2022-03-06].http://kns.cnki.net/kcms/detail/11.2206.TS.20210816.15 10.078.html.
[11] 孙亚利, 周文美, 黄永光, 等.以聚合乳清蛋白为壁材的苦荞黄酮微胶囊化及其品质分析[J].食品科学, 2020, 41(12):259-266.
SUN Y L, ZHOU W M, HUANG Y G, et al.Microencapsulation and quality analysis of tartary buckwheat flavonoids using polymerized whey protein as wall material[J].Food Science, 2020, 41(12):259-266.
[12] 雷雨晴, 王康平, 余雄伟, 等.卵白蛋白-菊糖-茶多酚微胶囊对石榴籽油稳定性的影响[J].食品科学, 2020, 41(21):58-65.
LEI Y Q, WANG K P, YU X W, et al.Stability of pomegranate seed oil loaded in ovalbumin-inluin-tea polyphenol microcapsules[J].Food Science, 2020, 41(21):58-65.
[13] 王枭, 孟官丽, 王齐蕾, 等.基于静电喷雾法萝卜硫素微胶囊的制备与表征[J].食品科学, 2021, 42(7):113-119.
WANG X, MENG G L, WANG Q L, et al.Preparation and characterization of sulforaphane microcapsules by electrospraying[J].Food Science, 2021, 42(7):113-119.
[14] 廖霞, 杨小兰, 李瑶, 等.槲皮素微胶囊的贮藏稳定性及抗氧化活性[J].食品科学, 2017, 38(1):60-66.
LIAO X, YANG X L, LI Y, et al.Storage stability and antioxidant activity of quercetin microcapsules[J].Food Science, 2017, 38(1):60-66.
[15] 康明, 孙蓉, 谢克难, 等.纳米碳酸钙干燥方式的研究[J].四川大学学报(工程科学版), 2004, 36(1):57-60.
KANG M, SUN R, XIE K N, et al.Study on drying processes of nanosized calcium carbonate[J].Journal of Sichuan University (Engineering Science Edition), 2004, 36(1):57-60.
[16] 尹乐斌, 刘桠丽, 何平, 等. 低苦味多肽固体饮料的制备及其体外模拟消化研究[J]. 食品与发酵工业, 2023, 49(4):189-195.
YIN L B, LIU Y L, HE P, et al. Preparation of low-bitter polypeptide solid beverage and study on its simulated digestion in vitro[J]. Food and Fermentation Industries, 2023, 49(4):189-195.
[17] 黄珊, 刘嘉, 李贵华, 等.壁材对方竹叶黄酮微胶囊结构及抗氧化性能的影响[J].食品工业科技, 2021, 42(22):55-61.
HUANG S, LIU J, LI G H, et al.Effects of wall materials on the structure and antioxidant activities of the bamboo leaves flavonoids microcapsules[J].Science and Technology of Food Industry, 2021, 42(22):55-61.
[18] 石泽栋, 蒋雅萍, 孙英杰, 等.牛至精油微胶囊的制备、表征及在杏贮藏期的抑菌效果[J].食品科学, 2021, 42(11):186-194.
SHI Z D, JIANG Y P, SUN Y J, et al.Preparation and characterization of oregano essential oil microcapsules and its effect on quality preservation of apricot fruit during storage[J].Food Science, 2021, 42(11):186-194.
[19] 毛莹, 帅晓艳, 王惠玲, 等.基于内源乳化法和喷雾干燥优化制备花色苷微胶囊及其稳定性分析[J].食品科学, 2020, 41(2):267-275.
MAO Y, SHUAI X Y, WANG H L, et al.Preparation and stability evaluation of anthocyanin microcapsules by emulsification/internal gelation with optimized spray drying[J].Food Science, 2020, 41(2):267-275.
[20] 赵彦巧, 王月, 孟翔宇, 等.喷雾干燥法制备玫瑰茄花色苷微胶囊条件优化[J].福建农业学报, 2021, 36(1):104-114.
ZHAO Y Q, WANG Y, MENG X Y, et al.Optimized preparation of spray-dried anthocyanins microcapsules[J].Fujian Journal of Agricultural Sciences, 2021, 36(1):104-114.
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