Food and Fermentation Industries >

2023 , Vol. 49 >Issue 9: 252 - 258

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.032556

Changes of endogenous protease activity and water content in Larimichthys polyactis during low temperature storage

  • MA Xinyue ,
  • HAN Yue ,
  • DENG Shanggui ,
  • ZHENG Bin ,
  • ZHANG Xiaojun ,
  • YANG Zuisu ,
  • XU Dan
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  • 1(College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China)
    2(Zhejiang Marine Fisheries Research Institute, Zhoushan 316021, China)
    3(Key Laboratory of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province, Zhoushan 316021, China)

Received date: 2022-06-04

  Revised date: 2022-07-20

  Online published: 2023-06-05

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Abstract

Taking the small yellow croaker (Larimichthys polyactis) as the research object, the enzyme activities of cathepsin, α-glucosidase (AG) and β-acetylglucosaminidase (NAG) were measured by low-field nuclear magnetic resonance (LF-NMR), to measure moisture migration. Using texture and water-holding capacity as indicators, the law of enzyme activity, moisture migration, and quality change of L. polyactis meat were explored during frozen storage (0-200 d) at -18, -25 and -45 ℃ were discussed. The results showed that with the prolongation of freezing storage time, the hardness, springiness and chewiness of fish meat at three temperatures all showed a significant downward trend; cathepsin B, H and L showed a trend of first decreasing and then increasing, and the -45 ℃ group enzyme activity of the fish meat was always significantly lower than that of the other two groups; the activities of AG and NAG enzymes increased significantly; the total moisture content (A), bound moisture content (A21), and immobilized moisture content (A22) of fish meat decreased, while the free moisture content (A23) decreased. The water-holding capacity of the fish meat in the -45 ℃ group decreased the slowest. The results of this study showed that the ultra-low temperature (-45 ℃) of L. polyactis can better maintain the fish quality during long-term freezing process.

Key words： Larimichthys polyactis; cathepsin; moisture migration; ultra-low temperature; low-field nuclear magnetic resonance

Cite this article

MA Xinyue , HAN Yue , DENG Shanggui , ZHENG Bin , ZHANG Xiaojun , YANG Zuisu , XU Dan . Changes of endogenous protease activity and water content in Larimichthys polyactis during low temperature storage[J]. Food and Fermentation Industries, 2023 , 49(9) : 252 -258 . DOI: 10.13995/j.cnki.11-1802/ts.032556

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