Research progress on separation, purification, chemical properties, and bioactivity of polysaccharide from Bletilla striata

  • ZHU Beibei
Expand
  • (College of Food Engineering, Shangqiu Polytechnic, Shangqiu 476000, China)

Received date: 2022-08-03

  Revised date: 2022-08-16

  Online published: 2023-06-13

Abstract

As a traditional medicinal and food homologous plant, Bletilla striata has the effects of promoting muscle and hemostasis, and a long history of application. Studies have shown that Bletilla striata polysaccharide is its main functional active component, which has the functions of antioxidant, anti-inflammatory, hemostasis, immune regulation, gastrointestinal mucosal protection, etc. These biological activities are closely related to the chemical structure of Bletilla striata polysaccharide. The chemical structure of Bletilla striata polysaccharide is the basis of its biological activity, which is affected by many factors, such as monosaccharide composition, molecular weight, glycosidic bond connection mode, spatial conformation, and extraction, separation, and purification methods of Bletilla striata polysaccharide, etc., which could cause differences in polysaccharide structure. In this paper, the research progress of extraction, purification, structural characteristics, and biological activity of Bletilla striata polysaccharide was reviewed, and the structure-activity relationship between the structure and biological activity of Bletilla striata polysaccharide was clarified to further promote the application of Bletilla striata polysaccharide in functional food and biomedicine.

Cite this article

ZHU Beibei . Research progress on separation, purification, chemical properties, and bioactivity of polysaccharide from Bletilla striata[J]. Food and Fermentation Industries, 2023 , 49(10) : 343 -350 . DOI: 10.13995/j.cnki.11-1802/ts.033193

References

[1] 孔伟华, 徐建波, 崔琦, 等.白及化学成分、药理作用和白及多糖提取工艺的研究进展[J].中医药信息, 2021, 38(9):69-78.
KONG W H, XU J B, CUI Q, et al.Research progress in chemical components and pharmacological actions of Bletilla striata and extraction technology of its polysaccharide[J].Information on Traditional Chinese Medicine, 2021, 38(9):69-78.
[2] 瞿燕, 张晨, 廖祯诚, 等.白及多糖应用于医用生物材料的探讨[J].中药与临床, 2017, 8(5):54-58.
QU Y, ZHANG C, LIAO Z C, et al.Exploration on pharmaceutical applications of Bletilla striata polysaccharide in medical biomaterials[J].Pharmacy and Clinics of Chinese Materia Medica, 2017, 8(5):54-58.
[3] CHEN Z Y, CHENG L Z, HE Y C, et al.Extraction, characterization, utilization as wound dressing and drug delivery of Bletilla striata polysaccharide:A review[J].International Journal of Biological Macromolecules, 2018, 120:2 076-2 085.
[4] 吕洪乐, 张同华, 李倩.白芨多糖药理作用的研究进展[J].中国药房, 2015, 26(28):4 014-4 016.
LYU H L, ZHANG T H, LI Q, et al.Research progress on pharmacological effects of Bletilla striata polysaccharide[J].China Pharmacy, 2015, 26(28):4 014-4 016.
[5] JI X L, YIN M S, NIE H, et al.A review of isolation, chemical properties, and bioactivities of polysaccharides from Bletilla striata[J].BioMed Research International, 2020, 2020:5391379.
[6] 马子豪, 马婕, 吕金盈, 等.白及多糖在新型递药系统和生物材料中的应用进展[J].中国中药杂志, 2021, 46(18):4 666-4 673.
MA Z H, MA J, LYU J Y, et al.Progress in application of Bletilla striata polysaccharide in novel drug delivery systems and biomaterials[J].China Journal of Chinese Materia Medica, 2021, 46(18):4 666-4 673.
[7] 胡雨涛, 叶欣, 徐伟, 等.中药白芨止血作用的及其应用的研究进展[J].药物资讯, 2021, 10(3):152-157.
HU Y T, YE X, XU W, et al.Advances in the study of the hemostation effect and its application of Chinese medicine Bletilla striata[J].Pharmacy Information, 2021, 10(3):152-157.
[8] 李雪营, 林先燕, 梁黔生, 等.不同炮制方法对白芨多糖含量的影响[J].海峡药学, 2021, 33(2):51-52.
LI X Y, LIN X Y, LIANG Q S, et al.Effects of different processing methods on polysaccharide content of Bletilla striata[J].Strait Pharmaceutical Journal, 2021, 33(2):51-52.
[9] 李笑眉. 白芨多糖水凝胶促进糖尿病创面愈合的应用研究[D].大连:大连医科大学, 2021.
LI X M.Study on the application of Bletilla striata polysaccharide hydrogel in promoting the healing of diabetic wounds[D].Dalian:Dalian Medical University, 2021.
[10] 葛雯, 陈宏降, 罗益远, 等.白及多糖的研究进展[J].人参研究, 2021, 33(3):55-59.
GE W, CHEN H J, LUO Y Y, et al.Research progress on Bletilla striata polysaccharide[J].Ginseng Research, 2021, 33(3):55-59.
[11] XU D L, PAN Y C, CHEN J S.Chemical constituents, pharmacologic properties, and clinical applications of Bletilla striata[J].Frontiers in Pharmacology, 2019, 10:1168.
[12] 唐森, 梁冰娜, 覃逸明, 等.响应面优化白芨水溶性多糖提取工艺的研究[J].山东化工, 2019, 48(22):3-7.
TANG S, LIANG B N, QIN Y M, et al.Extraction process of polysaccharide in Bletilla striata optimized by response surface method[J].Shandong Chemical Industry, 2019, 48(22):3-7.
[13] 董建新, 俞涵, 姜伟, 等.响应面法优化白及多糖的提取和醇沉工艺[J].基因组学与应用生物学, 2019, 38(8):3 681-3 689.
DONG J X, YU H, JIANG W, et al.Optimization of extraction and alcohol precipitation process of Bletilla striata polysaccharides based on response surface methodology[J].Genomics and Applied Biology, 2019, 38(8):3 681-3 689.
[14] 吴梦晴. 白芨多糖的超声辅助提取工艺及抗氧化活性研究[J].巢湖学院学报, 2019, 21(6):95-101.
WU M Q.Ultrasound-assisted extraction process and antioxidant activity of Bletilla striata polysaccharide[J].Journal of Chaohu University, 2019, 21(6):95-101.
[15] 刘长命, 王洁.白芨多糖提取的工艺优化[J].商洛学院学报, 2018, 32(4):49-53.
LIU C M, WANG J.Extraction conditions optimization of polysaccharide from Bletilla striata[J].Journal of Shangluo University, 2018, 32(4):49-53.
[16] LI Q, LI K, HUANG S S, et al.Optimization of extraction process and antibacterial activity of Bletilla striata polysaccharides[J].Asian Journal of Chemistry, 2014, 26(12):3 574-3 580.
[17] 刘煜, 韩伟.超声辅助提取白芨多糖的研究[J].机电信息, 2017(32):35-40;59.
LIU Y, HAN W.Ultrasonic-assisted extraction of polysaecharide from Bleailla striata[J].Mechanical and Electrical Information, 2017(32):35-40;59.
[18] 何晓梅, 谷仿丽, 黄仁术, 等.白芨多糖超声波辅助提取工艺优化及体外抗氧化活性[J].皖西学院学报, 2017, 33(2):1-5.
HE X M, GU F L, HUANG R S, et al.Ultrasonic-assisted extraction and in vitro antioxidant activities evaluation of polysaccharides from Blettila striata[J].Journal of West Anhui University, 2017, 33(2):1-5.
[19] QU Y, LI C X, ZHANG C, et al.Optimization of infrared-assisted extraction of Bletilla striata polysaccharides based on response surface methodology and their antioxidant activities[J].Carbohydrate Polymers, 2016, 148:345-353.
[20] 吴诗惠, 王剑波, 开拓, 等.白芨多糖超声提取工艺及其抗氧化活性研究[J].世界中医药, 2020, 15(17):2 556-2 560.
WU S H, WANG J B, KAI T, et al.Study on ultrasonic extraction technology and antioxidant activity of Bletilla striata polysaccharides extraction process[J].World Chinese Medicine, 2020, 15(17):2 556-2 560.
[21] 朱富成, 罗书岚, 郑宣, 等.大别山白芨多糖酶法辅助提取及活性研究[J].天然产物研究与开发, 2020, 32(8):1 389-1 395;1 323.
ZHU F C, LUO S L, ZHENG X, et al.Enzyme-assisted extraction of polysaccharides of Ta-pieh Mountains Bletilla striata and its activities study[J].Natural Product Research and Development, 2020, 32(8):1 389-1 395;1 323.
[22] 韩伟, 彭茹洁, 赵少甫.PB试验-响应面优化白芨多糖的提取工艺研究[J].徐州工程学院学报(自然科学版), 2018, 33(4):19-26.
HAN W, PENG R J, ZHAO S F.Extraction optimization of total polysaecharide from Bletilla striataby response surface methodology with plackett-burman design[J].Journal of Xuzhou Institute of Technology (Natural Sciences Edition), 2018, 33(4):19-26.
[23] JI X L, PENG B X, DING H H, et al.Purification, structure and biological activity of pumpkin polysaccharides:A review[J].Food Reviews International, 2023,39(1):307-319.
[24] 冀晓龙, 尹明松, 侯春彦, 等.红枣多糖提取、分离纯化及生物活性研究进展[J].食品工业科技, 2020, 41(23):346-353;358.
JI X L, YIN M S, HOU C Y, et al.Recent advances in jujube (Zizyphus jujuba Mill.) polysaccharides:Extraction, isolation and purification and bioactivities[J].Science and Technology of Food Industry, 2020, 41(23):346-353;358.
[25] ZHU J X, GUO X X, GUO T T, et al.Novel pH-responsive and self-assembled nanoparticles based on Bletilla striata polysaccharide:Preparation and characterization[J].RSC Advances, 2018, 8(70):40 308-40 320.
[26] WANG Y R, HAN S W, LI R F, et al.Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata[J].International Journal of Biological Macromolecules, 2019, 122:628-635.
[27] CHEN J K, LYU L Y, LI Y, et al.Preparation and evaluation of Bletilla striata polysaccharide/graphene oxide composite hemostatic sponge[J].International Journal of Biological Macromolecules, 2019, 130:827-835.
[28] 戴洁, 周子明, 刘敬.苯酚-硫酸比色法检测白芨多糖含量的分析[J].中国处方药, 2021, 19(3):33-35.
DAI J, ZHOU Z M, LIU J.The analyzing of detecting Bletilla striata polysaccharide by phenol sulfuric acid colorimetry[J].Journal of China Prescription Drug, 2021, 19(3):33-35.
[29] 车向前, 陈芳, 常明泉, 等.白芨溶胶中植物蛋白的定性定量方法研究[J].实用药物与临床, 2018, 21(7):815-818.
CHE X Q, CHEN F, CHANG M Q, et al.Study on the qualitative and quantitative methods for plant protein in Rhizoma Bletillae sol[J].Practical Pharmacy and Clinical Remedies, 2018, 21(7):815-818.
[30] JI X L, PENG Q, YUAN Y P, et al.Isolation, structures and bioactivities of the polysaccharides from jujube fruit (Ziziphus jujuba Mill.):A review[J].Food Chemistry, 2017, 227:349-357.
[31] LAI Y L, LIN Y Y, SADHASIVAM S, et al.Efficacy of Bletilla striata polysaccharide on hydrogen peroxide-induced apoptosis of osteoarthritic chondrocytes[J].Journal of Polymer Research, 2018, 25(2):49.
[32] 陈姿燕. 白芨源多糖的分离纯化、结构解析以及抗氧化活性研究[D].上海:上海交通大学, 2020.
CHEN Z Y.Isolation purification, structural analysis and antioxidant activity of polysaccharides from Bletilla striata[D].Shanghai:Shanghai Jiao Tong University, 2020.
[33] LIAO Z C, ZENG R, HU L L, et al.Polysaccharides from tubers of Bletilla striata:Physicochemical characterization, formulation of buccoadhesive wafers and preliminary study on treating oral ulcer[J].International Journal of Biological Macromolecules, 2019, 122:1 035-1 045.
[34] PENG Q, LI M, XUE F, et al.Structure and immunobiological activity of a new polysaccharide from Bletilla striata[J].Carbohydrate Polymers, 2014, 107:119-123.
[35] YUE L, WANG W, WANG Y, et al.Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways[J].International Journal of Biological Macromolecules, 2016, 89:376-388.
[36] WANG Y, LIU D, CHEN S J, et al.A new glucomannan from Bletilla striata:Structural and anti-fibrosis effects[J].Fitoterapia, 2014, 92:72-78.
[37] CHEN Z Y, ZHAO Y, ZHANG M K, et al.Structural characterization and antioxidant activity of a new polysaccharide from Bletilla striata fibrous roots[J].Carbohydrate Polymers, 2020, 227:115362.
[38] WANG C M, SUN J T, LUO Y, et al.A polysaccharide isolated from the medicinal herb Bletilla striata induces endothelial cells proliferation and vascular endothelial growth factor expression in vitro[J].Biotechnology Letters, 2006, 28(8):539-543.
[39] 王尚萍. 黄花白芨多糖结构解析及其抗肿瘤活性研究[D].西安:陕西师范大学, 2017.
WANG S P.Structural analysis and anti-tumor activity of polysaccharide from Bletilla striata[D].Xi′an:Shaanxi Normal University, 2017.
[40] 陈思思, 吴蓓, 谭婷, 等.白芨多糖BSP-1的分离纯化、结构表征及抗肿瘤活性研究[J].中草药, 2019, 50(8):1 921-1 926.
CHEN S S, WU B, TAN T, et al.Isolation, purification, structural characterization of Bletilla striata polysaccharides and its antitumor activity[J].Chinese Traditional and Herbal Drugs, 2019, 50(8):1 921-1 926.
[41] KONG L S, YU L, FENG T, et al.Physicochemical characterization of the polysaccharide from Bletilla striata:Effect of drying method[J].Carbohydrate Polymers, 2015, 125:1-8.
[42] ZHUANG Y, WANG L F, LIU C J, et al.A novel fiber from Bletilla striata tuber:Physical properties and application[J].Cellulose, 2019, 26(9):5 201-5 210.
[43] 刘文立, 莫海云.白芨多糖通过调节免疫作用抑制结肠癌CT26荷瘤小鼠肿瘤生长[J].中国免疫学杂志, 2021, 37(8):941-945.
LIU W L, MO H Y.Bletilla striata polysaccharide inhibits tumor growth in colon cancer CT26 tumor-bearing mice by regulating immunity[J].Chinese Journal of Immunology, 2021, 37(8):941-945.
[44] WANG J Q, HU S Z, NIE S P, et al.Reviews on mechanisms of in vitro antioxidant activity of polysaccharides[J].Oxidative Medicine and Cellular Longevity, 2016, 2016:5692852.
[45] ZHU F M, DU B, XU B J.Anti-inflammatory effects of phytochemicals from fruits, vegetables, and food legumes:A review[J].Critical Reviews in Food Science and Nutrition, 2018, 58(8):1 260-1 270.
[46] 周美, 万科, 马风伟, 等.响应面法优化白芨多糖酶解工艺及其抗氧化、免疫活性研究[J].食品研究与开发, 2020, 41(10):128-135.
ZHOU M, WAN K, MA F W, et al.Response surface methodology for optimization of enzymatic hydrolysis on Bletilla striata (Thunb.) Reiehb.f.polysaccharides and antioxidant, in vitro immunostimulatory activities analysis[J].Food Research and Development, 2020, 41(10):128-135.
[47] 郭琪, 孟烨, 赵阳, 等.白芨提取物对博莱霉素致大鼠肺纤维化的治疗作用[J].国际药学研究杂志, 2016, 43(3):518-523.
GUO Q, MENG Y, ZHAO Y, et al.Inhibitory effect of Bletilla striata extracts on bleomycin-induced pulmonary fibrosis in rats[J].Journal of International Pharmaceutical Research, 2016, 43(3):518-523.
[48] DIAO H J, LI X, CHEN J N, et al.Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages[J].Journal of Bioscience and Bioengineering, 2008, 105(2):85-89.
[49] ZHANG C, HE Y N, CHEN Z J, et al.Effect of polysaccharides from Bletilla striata on the healing of dermal wounds in mice[J].Evidence-based Complementary and Alternative Medicine:ECAM, 2019, 2019:9212314.
[50] LUO L, ZHOU Z, XUE J, et al.Bletilla striata polysaccharide has a protective effect on intestinal epithelial barrier disruption in TAA-induced cirrhotic rats[J].Experimental and Therapeutic Medicine, 2018, 16(3):1 715-1 722.
[51] 李雨芯, 余雪嫣, 黄雪.白芨多糖上调溃疡性结肠炎小鼠肠黏膜紧密连接蛋白occludin的表达[J].基础医学与临床, 2021, 41(7):941-945.
LI Y X, YU X Y, HUANG X.Bletilla striata polysaccharide up-regulates the expression of tight junction protein occludin in intestinal mucosa of mice with ulcerative colitis[J].Basic and Clinical Medicine, 2021, 41(7):941-945.
[52] LI X C, BAI G.In vivo and in vitro effects of Bletilla striata polysaccharide-loaded paclitaxel nanoparticles on human gastric cancer cells[J].Tropical Journal of Pharmaceutical Research, 2019, 18(1):13.
Outlines

/