Fermentation Engineering Principle and Technology is the core course of the national first-class undergraduate major of Jiangnan University, as well as the core course of Brewing Engineering major. This course aims to cultivate students' professional knowledge system in fermentation engineering, and focus on improving students′ engineering practice and ability to solve practical problems in modern fermentation industry. However, there are still some problems in the teaching process of this course, such as the brewing characteristics of the course system are not prominent enough; the students′ professional fear of difficulties is heavy; the theoretical study and industrial connection are poor; the evaluation system and feedback need to be improved; and the students′ awareness of innovation and practical ability cultivation is weak. Based on the above problems, we should give full play to the scientific and educational advantages of Jiangnan University in the field of brewing by restructuring the curriculum system covering the whole process of brewing technology, activating professional enthusiasm through the design of ideological and political elements, making theoretical learning and industrial in-depth integration through classic industrial case teaching, making continuous improvement of diversified evaluation and teaching feedback based on OBE(outcomes-based education) concepts, and extending the second classroom teaching of scientific and technological innovation competitions/industrial topics. Multi-dimensional strategies guarantee of the completion of curriculum training objectives. Through the above teaching reform and practice activities, the goal achievement of the course has been continuously improved, and the students have repeatedly won the awards of top discipline competitions. The course construction effectively realized the organic unity of knowledge teaching and innovation practice ability training, and continuously delivered innovative practical talents with engineering characteristics for the modern brewing industry.