The large-scale production of prodigiosin, which has biological activities such as antioxidant and antitumor, is of great importance for its research and development. A red pigment producing bacteria H04, isolated from the rhizosphere of Sophora japonica Linn., was identified as Serratia marcescens according to the 16S rDNA phylogenetic tree. The red pigment derived from H04 was determined as prodigiosin through UV and HPLC-MS. Single-factor and orthogonal experiments were used to optimize the fermentation conditions to improve the pigment yield. The optimum conditions were the fermentation medium using 15 g/L sucrose as carbon source, 0.5 g/L yeast extract powder, and 0.5 g/L ammonium sulfate as nitrogen source, 5 g/L NaCl, 0.75 g/L KCl, 10 g/L proline, and 0.5 g/L CaCO3; inoculum amount was 3%, 25 ℃, 180 r/min, 24 h. The optimized fermentation yielded 4.5 g/L of red pigment extract, with a 2.3-fold increase in yield compared to that before orthogonal optimization.
WANG Pengyu
,
XU Chenqi
,
PENG Han
,
JIANG Hongnian
,
LI He
,
HUO Yankun
,
REN Hong
. Isolation, identification, and optimization of fermentation conditions of a high-yielding prodigiosin Serratia marcescens H04[J]. Food and Fermentation Industries, 2023
, 49(11)
: 20
-26
.
DOI: 10.13995/j.cnki.11-1802/ts.032758
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