To study the hypolipidemic, antioxidant and hepatoprotective effects of fermentation broth made from hawthorn, Cassia tora, and Pueraria lobata, HPLC-MS/MS was used for qualitative and quantitative analysis of the components of broth. Mice were fed a high-fat diet for modeling, and after successful modeling, different doses of fermentation broth(treatment group) and simvastatin(positive control) were administered by gavage for 4 weeks. The concentration of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and malondialdehyde (MDA); the activity of superoxide dismutase (SOD), alanine transaminase (ALT) and aspartate transaminase (AST) were compared. The pathological results of liver and kidney tissues were compared and analyzed. The results showed that after 4 weeks of intragastric administration, compared with the high-fat model group, the body weight of mice in each treatment group recovered significantly (P<0.01). Compared with blank control, the concentrations of TC and TG in high-fat model group significantly increased (P<0.01). The concentrations of TG, TC, and LDL-C in treatment group significantly decreased (P<0.01), while that of HDL-C significantly increased (P<0.01). The activity of SOD was significantly increased (P<0.01), while the content of MDA, the activity of AST and ALT significantly decreased (P<0.01). Fermentation broth eliminated fat particles in live with a dose-dependent effect. Fermentation broth was less toxic to kidney. Kidneys of the high-fat model were more tolerant, with no significant damage to kidneys. The positive control group was also more effective, but not as effective as the fermentation broth. The fermentation broth has certain lipid-regulating, antioxidant, and hepatoprotective effects, and can better restore the fatty liver of hyperlipidemic mice, with has a quantitative-effect relationship, and its hypolipidemic effect is better than that of the positive control.
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