Effects of different mixed fermentation methods on the characteristic flavor of fermented soymilk

  • ZONG Lina ,
  • QU Hengxian ,
  • ZHANG Longfei ,
  • LIU Yang ,
  • LU Maolin ,
  • WANG Wenqiong ,
  • WA Yunchao ,
  • GU Ruixia
Expand
  • (Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou 225000, China)

Received date: 2022-09-02

  Revised date: 2022-10-06

  Online published: 2023-06-30

Abstract

In this study, the effect of different mixed fermentation methods on the flavor of fermented soymilk was studied. The volatile flavor of fermented soymilk under different mixed fermentation methods were analyzed by sensory score. The results showed that 40 kinds of volatile flavor compounds were detected in fermented mixed soymilk samples, among them, nine ketones and four esters were the main compounds that give fermented soymilk an aromatic smell, while six aldehydes were the main compounds that make fermented soymilk produce a bad flavor. The fermented milk was firstly acidified at 4 ℃ for 24 h, and then it was mixed with soymilk at a mass ratio of 1.5∶1. Under this condition, the acetoin, 2-heptanone, 1-nonanol, (E)-2-heptanaldehyde and (E)-2-octenal were produced to give good creamy flavor and soft aromatic flavor, it can cover up the bad flavor and give the best sensory score, and the sensory score was the highest.

Cite this article

ZONG Lina , QU Hengxian , ZHANG Longfei , LIU Yang , LU Maolin , WANG Wenqiong , WA Yunchao , GU Ruixia . Effects of different mixed fermentation methods on the characteristic flavor of fermented soymilk[J]. Food and Fermentation Industries, 2023 , 49(11) : 102 -110 . DOI: 10.13995/j.cnki.11-1802/ts.033522

References

[1] YANG Y, WANG B, FU Y, et al.HS-GC-IMS with PCA to analyze volatile flavor compounds across different production stages of fermented soybean whey tofu[J].Food Chemistry, 2021, 346:128880.
[2] LIU P, XIANG Q, GAO L, et al.Effects of different fermentation strains on the flavor characteristics of fermented soybean curd[J].Journal of Food Science, 2019, 84(1):154-164.
[3] 孙畅, 安彬, 杨柳, 等.复合益生菌发酵全豆豆乳的研制及储存稳定性研究[J].食品科技, 2019, 44(12):66-73.
SUN C, AN B, YANG L, et al.Study on preparation and storage stability of whole bean milk fermented by complex probiotics[J].Food Science and Technology, 2019, 44(12):66-73.
[4] 郭帅, 韩之皓, 白梅, 等.嗜热链球菌S10复合植物乳杆菌P-8发酵豆乳中挥发性风味物质的SPME-GC-MS分析[J].中国食品学报, 2020, 20(10):268-279.
GUO S, HAN Z H, BAI M, et al.Analysis of fermented soybean milk produced by Streptococcus thermophilus S10 compound Lactobacillus plantarum P-8 by SPME-GC-MS[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(10):268-279.
[5] PENG X H, LIAO Y, REN K Y, et al.Fermentation performance, nutrient composition, and flavor volatiles in soy milk after mixed culture fermentation[J].Process Biochemistry, 2022, 121:286-297.
[6] KANEKO S, KUMAZAWA K, NISHIMURA O.Studies on the key aroma compounds in soy milk made from three different soybean cultivars[J].Journal of Agricultural and Food Chemistry, 2011, 59(22):12 204-12 209.
[7] DU L, RO K S, ZHANG Y J, et al.Effects of Lactiplantibacillus plantarum X7021 on physicochemical properties, purines, isoflavones and volatile compounds of fermented soymilk[J].Process Biochemistry, 2022, 113:150-157.
[8] 刘婷, 赵良忠, 周小虎, 等.酸豆奶发酵过程中大豆异黄酮及风味物质变化规律[J].食品与发酵工业, 2022, 48(6):181-187.
LIU T, ZHAO L Z, ZHOU X H, et al.Changes of soybean isoflavones and flavor substances in fermented soybean milk[J].Food and Fermentation Industries, 2022, 48(6):181-187.
[9] 徐寅, 黄玉军, 陈霞, 等.牛乳含量对发酵豆乳风味成分的影响[J].食品科学, 2013, 34(4):1-5.
XU Y, HUANG Y J, CHEN X, et al.Influence of milk content on flavor compounds in fermented soymilk[J].Food Science,2013, 34(4):1-5.
[10] ZHENG Y, FEI Y T, YANG Y, et al.A potential flavor culture: Lactobacillus harbinensis M1 improves the organoleptic quality of fermented soymilk by high production of 2,3-butanedione and acetoin[J].Food Microbiology, 2020, 91:103540.
[11] 刘婷, 赵良忠, 李明, 等.乳酸菌发酵酸豆奶中不良风味物质研究进展[J].食品安全质量检测学报, 2021, 12(14):5 720-5 726.
LIU T, ZHAO L Z, LI M, et al.Research progress on off-flavor substancein fermented soybean milk by lactic acid bacteria[J].Journal of Food Safety & Quality, 2021, 12(14):5 720-5 726.
[12] CAO J, ZHANG Z M,ZHANG Q S, et al.Effects of Bacillus coagulans GBI-30, 6086 as an adjunct starter culture on the production of yogurt[J].Food Research International, 2022, 160:111398.
[13] 陈佩, 党辉, 贺国旗, 等.一株干酪乳杆菌生物学特性及其发酵豆乳的研究[J].食品研究与开发, 2017, 38(17):17-21.
CHEN P, DANG H, HE G Q, et al.Study on the biological characteristics of Lactobacillus casei and application in the fermented soymilk produce[J].Food Research and Development, 2017, 38(17):17-21.
[14] 李婷, 田佳乐, 刘洋, 等.保加利亚乳杆菌的筛选及其发酵乳中的风味物质[J].中国食品学报, 2021, 21(3):315-323.
LI T, TAIN J L, LIU Y, et al.Screening of Lactobacillus delbrueckii subsp.bulgaricus and its flavor substances in fermented milk[J].Chinese Journal of Food, 2021, 21(3):315-323.
[15] 张媛, 潘潇, 刘文君, 等.植物乳杆菌P9接种量对凝固型发酵乳贮藏特性的影响[J].中国乳品工业, 2022, 50(2):21-24.
ZHANG Y, PAN X, LIU W J, et al.Effect of Lactobacillus plantarum P9 inoculation amount on the storage characteristics of coagulated fermented milk[J].China Dairy Industry, 2022, 50(2):21-24.
[16] 王磊. 酸乳用乳酸菌的筛选与应用研究[D].扬州:扬州大学, 2021.
WANG L.Study on screening and application of lactic acid bacteria for yoghurt[D].Yangzhou:Yangzhou University, 2021.
[17] 杨淑娟, 苗壮壮, 史嘉琪, 等.乳双歧杆菌Probio-M8对发酵乳风味的影响及应用评价[J].中国食品学报, 2022, 22(7):256-266.
YANG S J, MIAO Z Z, SHI J Q, et al.Effect of Bifidobacterium lactis Probio-M8 on fermented milk flavor and its application evaluation[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(7):256-266.
[18] 任为一, 陈海燕, 李婷, 等.嗜热链球菌发酵乳中挥发性风味物质的GC-MS指纹图谱[J].中国食品学报, 2020, 20(9):190-202.
REN W Y, CHEN H Y, LI T, et al.Analysis of volatile flavor compounds in fermented milk with Streptococcus thermophilus by gas chromatographic fingerprints[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(9):190-202.
[19] TIAN H X, SHI Y H, ZHANG Y, et al.Screening of aroma-producing lactic acid bacteria and their application in improving the aromatic profile of yogurt[J].Journal of Food Biochemistry, 2019, 43(10):e12837.
[20] 王艺会, 蔡淼, 张敏, 等.添加酪蛋白水解肽改善酸乳的加工特性[J].食品科学, 2022, 43(12):34-41.
WANG Y H, CAI M, ZHANG M, et al.Adding casein hydrolytic peptides to improve the processing characteristics of yogurt[J].Food Science, 2022, 43(12):34-41.
[21] 丹彤, 乔少婷, 田佳乐.具有良好风味嗜热链球菌的筛选及其产香特性分析[J].食品工业科技, 2021, 42(16):99-105.
DAN T, QIAO S T, TIAN J L.Screening of Streptococcus thermophilus with good flavor and analysis of its aroma production characteristics[J].Science and Technology of Food Industry, 2021, 42(16):99-105.
[22] 李彤, 彭珍, 熊涛.乳酸菌发酵对复合豆乳饮料营养成分、香气成分及抗氧化活性的影响[J].食品与发酵工业, 2018, 44(4):111-118.
LI T, PENG Z, XIONG T.Effects of lactic acid bacteria on nutritional components, aroma components and antioxidant activity of compound soybean milk[J].Food and Fermentation Industries, 2018, 44(4):111-118.
[23] WANG Y W, GUO H C, WU A D, et al.Multiple-strain Lactobacillus-fermented soymilk with antioxidant capacity and delicate flavour[J].International Journal of Food Science & Technology, 2021, 56(11):6 052-6 061.
[24] 韩之皓, 郭帅, 黄天, 等.复合益生菌发酵不同基料乳酸菌饮料中挥发性代谢物差异分析[J].中国食品学报, 2021, 21(3):300-314.
HAN Z H, GUO S, HUANG T, et al.Difference analysis of volatile flavor metabolite in Lactobacillus beverage fermented with complex probiotics[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(3):300-314.
[25] DAN T, CHEN H Y, LI T, et al.Influence of Lactobacillus plantarum P-8 on fermented milk flavor and storage stability[J].Frontiers in Microbiology, 2019, 9:3133.
[26] 丹彤, 张和平.发酵乳中风味物质的研究进展[J].中国食品学报, 2018, 18(11):287-292.
DAN T, ZHANG H P.The research progress on flavor compounds in fermented milk[J].Journal of Chinese Institute of Food Science and Technology, 2018, 18(11):287-292.
[27] 王磊, 宗丽娜, 高宗露, 等.复合乳酸菌发酵酸乳贮藏期稳定性及其风味物质的研究[J].食品与发酵工业, 2021, 47(12):55-62.
WANG L, ZONG L N, GAO Z L, et al.Stability and flavor compounds of yoghurt fermented by composite lactic acid bacteria during storage[J].Food and Fermentation Industries, 2021, 47(12):55-62.
Outlines

/

〈 〉