It is crucial to develop new antimicrobial agents since the prevalence of pathogenic bacteria and antibiotic resistance poses great challenges to human health.To develop a simple method on synthesis of nanosized antibacterial agents with broad-spectrum antibacterial properties.Hydrothermal method was developed to synthesize the catechin functionalized ZnO/Ag nanocomposites wherein the catechin was regarded as the reducing agent and stabilizer.The morphology and structure of the prepared nanocomposites were characterized by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscope (TEM), energy dispersive spectroscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy (FTIR).The antibacterial activity was also tested.The results of UV-vis and TEM showed that silver nanoparticles dot on the surface of ZnO nanorods to form nanocomposites.The FTIR measurement confirmed that there was a strong interaction between catechin and nanocomposites, indicating that the successful synthesis of hydrophilic catechin functionalized ZnO/Ag nanocomposites.The antibacterial test results showed that the catechin-ZnO/Ag nanocomposites exerted strong bacteriostatic effect on Escherichia coli, Staphylococcus aureus and Candida albicans.The catechin-ZnO/Ag nanocomposites synthesized by the hydrothermal method possess broad-spectrum antibacterial properties, and are expected to be a new antibacterial agent to solve the problem of bacterial infection and antibiotic resistance.
[1] BEHZADNIA S, DAVOUDI A, REZAI M S, et al.Nosocomial infections in pediatric population and antibiotic resistance of the causative organisms in north of Iran[J].Iranian Red Crescent Medical Journal, 2014, 16(2):e14562.
[2] AMIN D H, ABOLMAATY A.Efficacy assessment of various natural and organic antimicrobials against Escherichia coli O157:H7, Salmonella enteritidis and Listeria monocytogenes[J].Bulletin of the National Research Centre, 2020, 44(1):172.
[3] CHEN R R, HAN Z H, HUANG Z L, et al.Antibacterial activity, cytotoxicity and mechanical behavior of nano-enhanced denture base resin with different kinds of inorganic antibacterial agents[J].Dental Materials Journal, 2017, 36(6):693-699.
[4] ALAVI M, KARIMI N, VALADBEIGI T.Antibacterial, antibiofilm, antiquorum sensing, antimotility, and antioxidant activities of green fabricated Ag, Cu, TiO2, ZnO, and Fe3O4 NPs via Protoparmeliopsis muralis lichen aqueous extract against multi-drug-resistant bacteria[J].ACS Biomaterials Science & Engineering, 2019, 5(9):4 228-4 243.
[5] ADEYEMI D K, ADELUOLA A O, AKINBILE M J, et al.Green synthesis of Ag, Zn and Cu nanoparticles from aqueous extract of Spondias mombin leaves and evaluation of their antibacterial activity[J].African Journal of Clinical and Experimental Microbiology, 2020, 21(2):106-113.
[6] LUNGU M V, VASILE E, LUCACI M, et al.Investigation of optical, structural, morphological and antimicrobial properties of carboxymethyl cellulose capped Ag-ZnO nanocomposites prepared by chemical and mechanical methods[J].Materials Characterization, 2016, 120:69-81.
[7] RAJITH KUMAR C R, BETAGERI V S, NAGARAJU G, et al.Synthesis of core/shell (ZnO/Ag) nanoparticles using Calotropis gigantea and their applications in photocatalytic and antibacterial studies[J].Journal of Inorganic and Organometallic Polymers and Materials, 2020, 30(9):3 410-3 417.
[8] ZHENG K Y, SETYAWATI M I, LEONG D T, et al.Antimicrobial silver nanomaterials[J].Coordination Chemistry Reviews, 2018, 357:1-17.
[9] MIRZAEI H, DARROUDI M.Zinc oxide nanoparticles:Biological synthesis and biomedical applications[J].Ceramics International, 2017, 43(1):907-914.
[10] AHMAD W, KALRA D.Green synthesis, characterization and anti microbial activities of ZnO nanoparticles using Euphorbia hirta leaf extract[J].Journal of King Saud University-Science, 2020, 32:2 358-2 364.
[11] ZHAO B H, DENG S X, LI J C, et al.Green synthesis, characterization and antibacterial study on the catechin-functionalized ZnO nanoclusters[J].Materials Research Express, 2021, 8(2):025006.
[12] 邢雅艳, 史宇哲, 邓世贤, 等.儿茶素-银纳米复合材料的制备及其应用[J].应用化学, 2020, 37(9):1 062-1 068.
XING Y Y, SHI Y Z, DENG S X, et al.Preparation and application of catechin-silver nanocomposites[J].Chinese Journal of Applied Chemistry, 2020, 37(9):1 062-1 068.
[13] BAE J, KIM N, SHIN Y, et al.Activity of catechins and their applications[J].Biomedical Dermatology, 2020, 4(1):8.
[14] PENSADO-LÓPEZ A, FERNÁNDEZ-REY J, REIMUNDE P, et al.Zebrafish models for the safety and therapeutic testing of nanoparticles with a focus on macrophages[J].Nanomaterials, 2021, 11(7):1784.
[15] PAL S, TAK Y K, SONG J M.Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli[J].Applied and Environmental Microbiology, 2007, 73(6):1 712-1 720.
[16] SHARMILA G, THIRUMARIMURUGAN M, MUTHUKUMARAN C.Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract:Characterization and evaluation of its antioxidant, bactericidal and anticancer activities[J].Microchemical Journal, 2019, 145:578-587.
[17] SONDI I, SALOPEK-SONDI B.Silver nanoparticles as antimicrobial agent:A case study on E.coli as a model for Gram-negative bacteria[J].Journal of Colloid and Interface Science, 2004, 275(1):177-182.
[18] LIU H R, LIU H, YANG J E, et al.Microwave-assisted one-pot synthesis of Ag decorated flower-like ZnO composites photocatalysts for dye degradation and NO removal[J].Ceramics International, 2019, 45(16):20 133-20 140.
[19] DENG Q, TANG H B, LIU G, et al.The fabrication and photocatalytic performances of flower-like Ag nanoparticles/ZnO nanosheets-assembled microspheres[J].Applied Surface Science, 2015, 331:50-57.
[20] XIA J, WANG D, LIANG P, et al.Vibrational (FT-IR, Raman) analysis of tea catechins based on both theoretical calculations and experiments[J].Biophysical Chemistry, 2020, 256:106282.
[21] TOMASZ D, KRYSTYNA S, RAFAŁ C, et al.The proapoptotic influence of AgNO3 on human keratinocytes and fibroblasts in vitro, the impact for burned patient management[J].Acta Poloniae Pharmaceutica, 2008, 65(5):515-519.
[22] ALI NAWAZ RANJHA M M, SHAFIQUE B, REHMAN A, et al.Biocompatible nanomaterials in food science, technology, and nutrient drug delivery:Recent developments and applications[J].Frontiers in Nutrition, 2022, 8:778155.
