Food and Fermentation Industries >

2023 , Vol. 49 >Issue 12: 278 - 284

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033093

Direct determination of 19 amino acids in raw materials of Moutai-flavor Jiuqu by ultra performance liquid chromatography-tandem mass spectrometry

  • QIAO Minsha ,
  • WANG Fan ,
  • SHAN Qimuge ,
  • QIAO Xiaona ,
  • BI Rongyu ,
  • ZHANG Changxia ,
  • LU Jun
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  • 1(Tasly Academy, Tasly Holding Group Co.Ltd., Tianjin 300410, China)
    2(Guizhou Guotai Distillery Co.Ltd., Renhuai 564501, China)

Received date: 2022-07-25

  Revised date: 2022-08-18

  Online published: 2023-07-13

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Abstract

An efficient and rapid method was established by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of 19 free amino acids in Moutai-flavor Daqu starter and its raw material -wheat.And the variation tendency of these amino acids in the production process of Moutai-flavor Daqu starter was preliminarily analyzed.The sample was for direct content determination after crushing, ultrasonic extraction, centrifugation and appropriate dilution.The results showed that the linear relationship of these 19 free amino acids was good within a specific range (R2≥0.998 3), the limit of detection (LOD) was 0.3-6.1 ng/mL, the limit of quantitation (LOQ) was 2.1-29.3 ng/mL, and the recovery rate was 84.5%-118.5%.The relative standard deviation values of repeatability and instrument precision were both less than 6.5% (n=6).The method has the advantages of high speed, high sensitivity and good selectivity and is suitable for the quantitative analysis of 19 free amino acids in Moutai-flavor Daqu starter and its raw material.

Key words： amino acids; determination; ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); Moutai-flavor Jiuqu

Cite this article

QIAO Minsha , WANG Fan , SHAN Qimuge , QIAO Xiaona , BI Rongyu , ZHANG Changxia , LU Jun . Direct determination of 19 amino acids in raw materials of Moutai-flavor Jiuqu by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2023 , 49(12) : 278 -284 . DOI: 10.13995/j.cnki.11-1802/ts.033093

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