To explore the difference in antioxidant capacity between astaxanthin from Haematococcus pluvialis (E-AST) and synthetic astaxanthin (S-AST), the effects of astaxanthin on cell viability, different active oxygen molecules, and cellular antioxidant activity (CAA) in HepG2 cells were analyzed after 24 h treatment. Results showed that E-AST and S-AST had no obvious toxic effects on HepG2 cells, and 10 μmol/L E-AST could significantly improve the viability of HepG2 cells. Fluorescence probe DCFH-DA identified that both of E-AST and S-AST could significantly decrease ROS content in HepG2 cells compared with the control group (P<0.05), except 5 μmol/L E-AST and 2.5 μmol/L S-AST. However, there was no significant difference between the two astaxanthin treatment groups with the same concentration (P>0.05). The detection results with DHR123 showed that the scavenging capacity of 5 μmol/L E-AST on hydrogen peroxide (H2O2) was significantly higher than that of S-AST (P<0.05). Furthermore, superoxide anion free radical (·O2-) specific probes DHE demonstrated that the ·O2- content decreased significantly exposed to 2.5 μmol/L S-AST (P<0.05), while other treatment groups had no significant change compared with the control group (P>0.05). The CAA of the two astaxanthins was increased does-dependent, but there was no significant difference between E-AST and S-AST at the same concentration (P>0.05). These results suggest that the antioxidant capacity of astaxanthin from two different sources is different in HepG2 cells by scavenging reactive oxygen species.
DING Rui
,
FENG Minglei
,
CHEN Aqin
. Evaluation of antioxidant capacity of astaxanthin from different sources based on HepG2 cells[J]. Food and Fermentation Industries, 2023
, 49(13)
: 172
-176
.
DOI: 10.13995/j.cnki.11-1802/ts.032263
[1] HIGUERA-CIAPARA I, FÉLIX-VALENZUELA L, GOYCOOLEA F M.Astaxanthin:A review of its chemistry and applications[J].Critical Reviews in Food Science and Nutrition, 2006, 46(2):185-196.
[2] AMBATI R R, PHANG S M, RAVI S, et al.Astaxanthin:Sources, extraction, stability, biological activities and its commercial applications:A review[J].Marine Drugs, 2014, 12(1):128-152.
[3] CAPELLI B, TALBOTT S, DING L.Astaxanthin sources:Suitability for human health and nutrition[J].Functional Foods in Health and Disease, 2019, 9(6):430-445.
[4] SIES H, JONES D P.Reactive oxygen species (ROS) as pleiotropic physiological signalling agents[J].Nature Reviews Molecular Cell Biology, 2020, 21(7):363-383.
[5] FASSETT R G, COOMBES J S.Astaxanthin:A potential therapeutic agent in cardiovascular disease[J].Marine Drugs, 2011, 9(3):447-465.
[6] KIM S H, LIM J W, KIM H.Astaxanthin prevents decreases in superoxide dismutase 2 level and superoxide dismutase activity in Helicobacter pylori-infected gastric epithelial cells[J].Journal of Cancer Prevention, 2019, 24(1):54-58.
[7] ISPADA J, RODRIGUES T A, RISOLIA P H B, et al.Astaxanthin counteracts the effects of heat shock on the maturation of bovine oocytes[J].Reproduction, Fertility, and Development, 2018, 30(9):1169-1179.
[8] KIM S H, KIM H.Inhibitory effect of astaxanthin on oxidative stress-induced mitochondrial dysfunction-a mini-review[J].Nutrients, 2018, 10(9):1137.
[9] 刘佳琳, 杜春梅.荧光探针法测定活性氧的研究进展[J].中国农学通报, 2018, 34(33):160-164.
LIU J L, DU C M.The detection of reactive oxygen with fluorescent probes:Research progress[J].Chinese Agricultural Science Bulletin, 2018, 34(33):160-164.
[10] MENG X Q, ZHANG W, ZHANG F, et al.Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells[J].Oncology Letters, 2016, 11(3):2145-2151.
[11] HU Y Y, SHEN L, LI L, et al.Astaxanthin protects against diabetic cardiomyopathy via activation of Akt pathway in H9c2 cells[J].Tropical Journal of Pharmaceutical Research, 2018, 17(11):2151-2156.
[12] SUDHARSHAN S J, DYAVAIAH M.Astaxanthin protects oxidative stress mediated DNA damage and enhances longevity in Saccharomyces cerevisiae[J].Biogerontology, 2021, 22(1):81-100.
[13] WOLFE K L, LIU R H.Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements[J].Journal of Agricultural and Food Chemistry, 2007, 55(22):8896-8907.
[14] LIM S R, KIM D W, SUNG J, et al.Astaxanthin inhibits autophagic cell death induced by bisphenol A in human dermal fibroblasts[J].Antioxidants (Basel, Switzerland), 2021, 10(8):1273.
[15] LI J J, WANG F, XIA Y J, et al.Astaxanthin pretreatment attenuates hepatic ischemia reperfusion-induced apoptosis and autophagy via the ROS/MAPK pathway in mice[J].Marine Drugs, 2015, 13(6):3368-3387.
[16] MACEDO R C, BOLIN A P, MARIN D P, et al.Astaxanthin addition improves human neutrophils function:In vitro study[J].European Journal of Nutrition, 2010, 49(8):447-457.
[17] RÉGNIER P, BASTIAS J, RODRIGUEZ-RUIZ V, et al.Astaxanthin from Haematococcus pluvialis prevents oxidative stress on human endothelial cells without toxicity[J].Marine Drugs, 2015, 13(5):2857-2874.
[18] YANG C, ZHANG L F, ZHANG H, et al.Rapid and efficient conversion of all-E-astaxanthin to 9Z-and 13Z-isomers and assessment of their stability and antioxidant activities[J].Journal of Agricultural and Food Chemistry, 2017, 65(4):818-826.
[19] VISIOLI F, ARTARIA C.Astaxanthin in cardiovascular health and disease:Mechanisms of action, therapeutic merits, and knowledge gaps[J].Food & Function, 2017, 8(1):39-63.
[20] DOSE J, MATSUGO S, YOKOKAWA H, et al.Free radical scavenging and cellular antioxidant properties of astaxanthin[J].International Journal of Molecular Sciences, 2016,17(1):103.
[21] NIU T T, XUAN R R, JIANG L G, et al.Astaxanthin induces the Nrf2/HO-1 antioxidant pathway in human umbilical vein endothelial cells by generating trace amounts of ROS[J].Journal of Agricultural and Food Chemistry, 2018, 66(6):1551-1559.
