Gas vesicles, which are composed of gas vesicle protein (Gvp), exist in Cyanobacteria, halophilic Archaea, and other microorganisms, providing buoyancy or stress resistance for cells. Although gvp gene cluster widely exists in Streptomyces strains, its function is still unclear. In this study, the endogenous gvpA, gvpO, and complete gene cluster gvpOAFGJLSK (gvp40587) were expressed in a transglutaminase gene deleted strain (smY2019Δtg) respectively to investigate their effects on the trypsin synthesis and the physiological status of the host. As indicated by enzyme activity analysis, overexpressing gvp40587 and gvpA increased the highest extracellular trypsin activity by 64.3% and 17.0%, respectively; the gvpO overexpression had little effect on trypsin activity. SDS-PAGE analysis showed that the trypsin bands (26 kDa) in the extracellular supernatant of gvp40587 and gvpA overexpression strains were significantly thicker than that of the control strain. In the strain over-expressing the complete gvp gene cluster, the transcriptional levels of the eight gvp genes were increased to different degrees. In addition, the intracellular ATP content and NADPH/NADP+ of the gvp40587-overexpressed strain increased 1.4 and 0.4 times than those of the control strain, respectively. These results showed that the overexpression of endogenous gvp gene cluster could improve the trypsin synthesis by S. mobaraensis, and the increases in energy metabolism and protein precursor synthesis may be the main reason for the increased yield. This is the first report regarding the important role of gvp gene in the enzyme production in S. mobaraensis, providing new ideas for the regulation of protein expression in Streptomyces.
SU Yingying
,
LIU Song
. Enhanced trypsin production in Streptomyces mobaraensis by overexpressing gas vesicle protein[J]. Food and Fermentation Industries, 2023
, 49(14)
: 1
-8
.
DOI: 10.13995/j.cnki.11-1802/ts.035479
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