Antioxidant effect and antiallergic activity of corn oligopeptides, wheat oligopeptides and their mineral chelate

  • LU Zhihao ,
  • ZHAO Xiaohan ,
  • PEI Chenhao ,
  • FENG Xiaowen ,
  • CHENG Qingli ,
  • GU Ruizeng ,
  • LI Guoming ,
  • LIU Wenying
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  • 1(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing Engineering Research Center of Protein and Functional Peptides, Beijing 100015, China)
    2(School of Biochemical, Beijing City University, Beijing 100083, China)

Received date: 2020-12-17

  Revised date: 2021-01-12

  Online published: 2023-08-30

Abstract

The antioxidant effect and antiallergic activity of corn oligopeptides, wheat oligopeptides, calcium-chelating corn oligopeptides, and ferrum-chelating wheat oligopeptides were studied based on the physicochemical property of two oligopeptides. The ability to scavenge DPPH radical and hydroxyl radical as well as the reductive power and hyaluronidase activity inhibition were analyzed. The antioxidant capacity analysis showed that the four samples had the strong antioxidant capacity, and the hydroxyl radical scavenging ratio of the two chelates was significantly higher than that of the corresponding oligopeptides. The analysis of antiallergic activity indicated that the hyaluronidase inhibition ratio of the chelates was much higher than that of the corresponding oligopeptides when the concentration was less than 20 mg/mL. There was a certain positive correlation between the antioxidant capacity and antiallergic activity of the sample because of the similarity between the results of hydroxyl radical test and the hyaluronidase inhibition test. However, the inhibition ratio of oligopeptides increased significantly at a concentration of 20-100 mg/mL, while the inhibition ratio of chelates tended to be flat. The chelates possess both strong antioxidant capacity and antiallergic activity when the concentration was less than 20 mg/mL, so the chelates can be further applied and developed in the food and medicine area.

Cite this article

LU Zhihao , ZHAO Xiaohan , PEI Chenhao , FENG Xiaowen , CHENG Qingli , GU Ruizeng , LI Guoming , LIU Wenying . Antioxidant effect and antiallergic activity of corn oligopeptides, wheat oligopeptides and their mineral chelate[J]. Food and Fermentation Industries, 2023 , 49(14) : 35 -40 . DOI: 10.13995/j.cnki.11-1802/ts.026488

References

[1] 李勇, 蔡木易.肽营养学[M].北京:北京大学医学出版社, 2007.
LI Y, CAI M Y.Peptide Nutrition[M].Beijing:Peking University Medical Press, 2007.
[2] ORTIZ-MARTINEZ M, GONZALEZ DE MEJIA E, GARCÍA-LARA S, et al.Antiproliferative effect of peptide fractions isolated from a quality protein maize, a white hybrid maize, and their derived peptides on hepatocarcinoma human HepG2 cells[J].Journal of Functional Foods, 2017, 34:36-48.
[3] WANG L Y, DING L, YU Z P, et al.Intracellular ROS scavenging and antioxidant enzyme regulating capacities of corn gluten meal -derived antioxidant peptides in HepG2 cells[J].Food Research International, 2016, 90:33-41.
[4] 周伟, 赵泽龙, 张丽萍.小麦低聚肽的结构及抗氧化能力分析[J].食品工业, 2017, 38(9):196-200.
ZHOU W, ZHAO Z L, ZHANG L P.Structure and antioxidant capacity of wheat oligopeptide[J].The Food Industry, 2017, 38(9):196-200.
[5] HUANG W H, SUN J, HE H, et al.Antihypertensive effect of corn peptides, produced by a continuous production in enzymatic membrane reactor, in spontaneously hypertensive rats[J].Food Chemistry, 2011, 128(4):968-973.
[6] MUGURUMA M, AHHMED A M, KATAYAMA K, et al.Identification of pro-drug type ACE inhibitory peptide sourced from porcine myosin B:Evaluation of its antihypertensive effects in vivo[J].Food Chemistry, 2009, 114(2):516-522.
[7] CAETANO-SILVA M E, NETTO F M, BERTOLDO-PACHECO M T, et al.Peptide-metal complexes:Obtention and role in increasing bioavailability and decreasing the pro-oxidant effect of minerals[J].Critical Reviews in Food Science and Nutrition, 2021,61(9):1 470-1 489.
[8] PALIKA R, MASHURABAD P C, NAIR M K, et al.Characterization of iron-binding phosphopeptide released by gastrointestinal digestion of egg white[J].Food Research International, 2015, 67:308-314.
[9] DE LA HOZ L, PONEZI A N, MILANI R F, et al.Iron-binding properties of sugar cane yeast peptides[J].Food Chemistry, 2014, 142:166-169.
[10] ZHAO L N, HUANG S L, CAI X X, et al.A specific peptide with calcium chelating capacity isolated from whey protein hydrolysate[J].Journal of Functional Foods, 2014, 10:46-53.
[11] 刘艳, 鲁军, 陈亮, 等.牡蛎肽锌螯合物的制备工艺研究[J].食品工业科技, 2016, 37(8):257-261.
LIU Y, LU J, CHEN L, et al.Preparation of oyster peptide-zinc chelates[J].Science and Technology of Food Industry, 2016, 37(8):257-261.
[12] 蒲传奋, 唐文婷.玉米醇溶蛋白肽钙螯合物的制备及其抑菌性能研究[J].食品科技, 2015, 40(12):246-250.
PU C F, TANG W T.Preparation and antibacterial properties of zein peptide-calcium chelate[J].Food Science and Technology, 2015, 40(12):246-250.
[13] 王子怀, 胡晓, 李来好, 等.肽-金属离子螯合物的研究进展[J].食品工业科技, 2014, 35(8):359-362.
WANG Z H, HU X, LI L H, et al.Research progress in peptide-mineral ion complexes[J].Science and Technology of Food Industry, 2014, 35(8):359-362.
[14] 汪婧瑜, 张业辉, 张友胜, 等.不同结构的乌鳢螯合肽对抗氧化活性的影响[J].中国食品学报, 2019, 19(10):93-99.
WANG J Y, ZHANG Y H, ZHANG Y S, et al.The effect of different structure of chelated peptides of Channa argus on antioxidant activity[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(10):93-99.
[15] 张智, 刘慧, 刘奇, 等.玉米肽-锌螯合物结构表征及抗氧化活性分析[J].食品科学, 2017, 38(3):131-135.
ZHANG Z, LIU H, LIU Q, et al.Structural characteristics and antioxidant activity of corn peptide-Zn complex[J].Food Science, 2017, 38(3):131-135.
[16] 中华人民共和国工业与信息化部. QB/T 5298—2018 小麦低聚肽粉[S].北京:中国轻工业出版社, 2018.
Ministry of Industry and Information Technology of the People′s Republic of China.QB/T 5298—2018 Oligopeptides powder of wheat[S].Beijing:China Light Industry Press, 2018.
[17] 中华人民共和国工业与信息化部. QB/T 4707—2014 玉米低聚肽粉[S].北京:中国轻工业出版社, 2014.
Ministry of Industry and Information Technology of the People’s Republic of China.QB/T 4707—2014 Oligopeptides powder of corn[S].Beijing:China Light Industry Press, 2014.
[18] 韦献雅, 殷丽琴, 钟成, 等.DPPH法评价抗氧化活性研究进展[J].食品科学, 2014, 35(9):317-322.
WEI X Y, YIN L Q, ZHONG C, et al.Advances in the DPPH radical scavenging assay for antioxidant activity evaluation[J].Food Science, 2014, 35(9):317-322.
[19] LIU X, SUN Z L, ZHANG M S, et al.Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus[J].Carbohydrate Polymers, 2012, 90(4):1 664-1 670.
[20] ZOVKO KONČIČ M, KREMER D, GRUZ J, et al.Antioxidant and antimicrobial properties of Moltkia petraea(Tratt.) Griseb.flower, leaf and stem infusions[J].Food and Chemical Toxicology, 2010, 48(6):1 537-1 542.
[21] 刘超, 孙培冬, 章姝敏.鸢尾苷的提取分离及其对透明质酸酶活性的影响[J].应用化工, 2012, 41(1):133-136.
LIU C, SUN P D, ZHANG S M.Extraction and separation of tectoridin and its influence on the activity of hyaluronidase[J].Applied Chemical Industry, 2012, 41(1):133-136.
[22] ARLIAN L G. Arthropod allergens and human health[J].Annual Review of Entomology, 2002, 47:395-433.
[23] METCALFE D D.Food hypersensitivity[J].Journal of Allergy and Clinical Immunology, 1984, 73(6):749-762.
[24] 方磊, 李国明, 徐珊珊, 等.牡蛎肽和三文鱼皮胶原肽低致敏性和抗过敏活性研究[J].食品与发酵工业, 2018, 44(9):91-97.
FANG L, LI G M, XU S S, et al.Study of low sensitization and antiallergic activity of oyster peptides and salmon skin collagen peptides[J].Food and Fermentation Industries, 2018, 44(9):91-97.
[25] MIURA K, MORISHITA Y, MATSUNO H, et al.Anti-allergic activity of monoacylated ascorbic acid 2-glucosides[J].Molecules, 2017, 22(12):2202.
[26] 侯安存. 关于补钙的研究进展[J].临床和实验医学杂志, 2016, 15(18):1 862-1 865.
HOU A C.Research progress in calcium supplement[J].Journal of Clinical and Experimental Medicine, 2016, 15(18):1 862-1 865.
[27] 王方海, 赵维, 陈建芳, 等.补铁剂研究进展[J].药学进展, 2016, 40(9):680-688.
WANG F H, ZHAO W, CHEN J F, et al.Research progress in iron supplement[J].Progress in Pharmaceutical Sciences, 2016, 40(9):680-688.
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