This research used ultrasonic-assisted technique to prepare thyme essential oil nanoemulsion with different concentrations and droplet sizes, then explored the inhibitory effects of concentration and droplet size of nanoemulsion on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis. It was observed by transmission electron microscopy that the nanoemulsions were perfectly spherical with the average droplet size of 50 nm, and were comparable to the results measured by particle size analyzer. Fourier transform infrared spectroscopy showed that the change of ultrasonic power could adjust the droplet size of the nanoemulsion but had no effect on the chemical structure of its components. Antimicrobial activity research proved that the concentration of thyme essential oil nanoemulsion had a significant effect on the antibacterial of different strains (P<0.05), while the droplet size had difference on antibacterial effect of different strains. P. aeruginosa had the most significant effect (P<0.05), while that of E. coli was not as apparent as that of P. aeruginosa. The results showed that the optimal combination of the concentration and droplet size of the thyme essential oil nanoemulsion could enhance its antibacterial effect.
XUE Siya
,
ZHANG Runfeng
,
WANG Qing
,
CHEN Shan
. Antibacterial activity of thyme essential oil nanoemulsion with different concentration and droplet size[J]. Food and Fermentation Industries, 2023
, 49(14)
: 67
-72
.
DOI: 10.13995/j.cnki.11-1802/ts.033074
[1] LI C P, PRESCOTT B, CHI L L, et al.Antiviral and antibacterial activity of thymus extracts[J].Proceedings of the Society for Experimental Biology and Medicine, 1963, 114:504-509.
[2] 袁丽雪, 赵愉涵, 孙斐, 等.香茅精油处理对马铃薯的抑芽作用研究[J].食品与发酵工业, 2022,48(23):256-263.
YUAN L X, ZHAO Y H, SUN F,et al.Inhibition effect of citronella essential oil on potato tuber sprout[J].Food and Fermentation Industries, 2022,48(23):256-263.
[3] PANDEY A K, CHÁÁVEZ-GONZÁLEZ M L, SILVA A S, et al.Essential oils from the genus Thymus as antimicrobial food preservatives:Progress in their use as nanoemulsions-a new paradigm[J].Trends in Food Science & Technology, 2021, 111:426-441.
[4] OUAKOUAK H, BENARFA A, MESSAOUDI M, et al.Biological properties of essential oils from thymus algeriensis boiss[J].Plants,2021, 10(4):786.
[5] ARTIGA-ARTIGAS M, GUERRA-ROSAS M I, MORALES-CASTRO J, et al.Influence of essential oils and pectin on nanoemulsion formulation:A ternary phase experimental approach[J].Food Hydrocolloids, 2018, 81:209-219.
[6] OZOGUL Y, KULEY BOĞA E, AKYOL I, et al.Antimicrobial activity of thyme essential oil nanoemulsions on spoilage bacteria of fish and food-borne pathogens[J].Food Bioscience, 2020, 36:100635.
[7] KOMAIKO J S, MCCLEMENTS D J.Formation of food-grade nanoemulsions using low-energy preparation methods:A review of available methods[J].Comprehensive Reviews in Food Science and Food Safety, 2016, 15(2):331-352.
[8] YANG Z H, HE Q, ISMAIL B B, et al.Ultrasonication induced nano-emulsification of thyme essential oil:Optimization and antibacterial mechanism against Escherichia coli[J].Food Control, 2022, 133:108609.
[9] GUO M M, ZHANG L J, HE Q, et al.Synergistic antibacterial effects of ultrasound and thyme essential oils nanoemulsion against Escherichia coli O157:H7[J].Ultrasonics Sonochemistry, 2020, 66:104988.
[10] HE Q, ZHANG L J, SONG L Y, et al.Inactivation of Staphylococcus aureus using ultrasound in combination with thyme essential oil nanoemulsions and its synergistic mechanism[J].LWT, 2021, 147:111574.
[11] HE Q, LIU D H, ASHOKKUMAR M, et al.Antibacterial mechanism of ultrasound against Escherichia coli:Alterations in membrane microstructures and properties[J].Ultrasonics Sonochemistry, 2021, 73:105509.
[12] ASWATHANARAYAN J B, VITTAL R R.Nanoemulsions and their potential applications in food industry[J].Frontiers in Sustainable Food Systems, 2019, 3:95.
[13] KAH M, BEULKE S, TIEDE K, et al.Nanopesticides:State of knowledge, environmental fate, and exposure modeling[J].Critical Reviews in Environmental Science and Technology, 2013, 43(16):1 823-1 867.
[14] MOGHIMI R, GHADERI L, RAFATI H, et al.Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E.coli[J].Food Chemistry, 2016, 194:410-415.
[15] AHARI H, NASIRI M.Ultrasonic technique for production of nanoemulsions for food packaging purposes:A review study[J].Coatings, 2021, 11(7):847.
[16] NOMAN A, JIANG Q X, XU Y S, et al.Effects of ultrasonic, microwave, and combined ultrasonic-microwave pretreatments on the enzymatic hydrolysis process and protein hydrolysate properties obtained from Chinese sturgeon (Acipenser sinensis)[J].Journal of Food Biochemistry, 2020, 44(8):e13292.
[17] WANG Y B, CEN C N, CHEN J, et al.Nano-emulsification improves physical properties and bioactivities of litsea cubeba essential oil[J].LWT, 2021, 137:110361.
[18] OMID A, HODA J.Intensification process in thyme essential oil nanoemulsion preparation based on subcritical water as green solvent and six different emulsifiers[J].Green Processing and Synthesis, 2021, 10(1):430-439.
[19] JADHAV A J, HOLKAR C R, KAREKAR S E, et al.Ultrasound assisted manufacturing of paraffin wax nanoemulsions:Process optimization[J].Ultrasonics Sonochemistry, 2015, 23:201-207.
[20] FADLI M, CHEVALIER J, BOLLA J M, et al.Thymus maroccanus essential oil, a membranotropic compound active on Gram-negative bacteria and resistant isolates[J].Journal of Applied Microbiology, 2012, 113(5):1 120-1 129.
[21] GUERRA-ROSAS M I, MORALES-CASTRO J, CUBERO-MÁRQUEZ M A, et al.Antimicrobial activity of nanoemulsions containing essential oils and high methoxyl pectin during long-term storage[J].Food Control, 2017, 77:131-138.
[22] CHANG Y H, MCLANDSBOROUGH L, MCCLEMENTS D J.Physical properties and antimicrobial efficacy of thyme oil nanoemulsions:Influence of ripening inhibitors[J].Journal of Agricultural and Food Chemistry, 2012, 60(48):12 056-12 063.
[23] LIAO W, DUMAS E, GHNIMI S, et al.Effect of emulsifier and droplet size on the antibacterial properties of emulsions and emulsion-based films containing essential oil compounds[J].Journal of Food Processing and Preservation, 2021, 45(12):e16072.
