Effect of ultrasound-assisted enzyme extraction on the protein degradation and biological of peptides in camel skin collagen

  • HE Jing ,
  • SHI Rui ,
  • Jirimutu
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  • 1(College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)
    2(China-Mongolia Joint Laboratory of Biopolymer Application “One Belt One Road”, Hohhot 010018, China)

Received date: 2022-08-16

  Revised date: 2022-08-30

  Online published: 2023-08-31

Abstract

In this study, the hydrolysis characteristics of collagen obtained by ultrasonic assisted enzymatic extraction and traditional enzymatic extraction were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, particle size distribution, and liquid chromatography tandem mass spectrometry based on simulated gastrointestinal digestion. Furthermore, molecular docking was carried out to screen the most potential bioactive peptides with high binding capacity and investigate the binding site and binding mechanism with target protein. The results showed that ultrasonic assisted enzyme extraction promoted the degradation of camel skin collagen, and its molecular mass of peptide fragments was distributed in the range of 500-1 500 Da. Through the virtual screening technology, it was confirmed that the ultrasonic-assisted enzymatic extraction could produce more potential bioactive peptides, mainly ACE inhibition. The most potential ACE inhibitory peptide LSFLF was obtained based on molecular docking. It could interact with the key amino acid residues with the active site of ACE. The results provide a new idea for further processing of camel skin and identification and preparation of bioactive peptides.

Cite this article

HE Jing , SHI Rui , Jirimutu . Effect of ultrasound-assisted enzyme extraction on the protein degradation and biological of peptides in camel skin collagen[J]. Food and Fermentation Industries, 2023 , 49(15) : 153 -159 . DOI: 10.13995/j.cnki.11-1802/ts.033338

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