Food and Fermentation Industries >

2023 , Vol. 49 >Issue 17: 202 - 208

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035762

A novel extraction method mediated with n-hexane and calcium chloride to concurrently extract phycocyanin and lipids from unbroken Spirulina platensis

  • GUO Xu ,
  • WEI Dengxiao ,
  • ZHONG Cairong ,
  • LAN Ying ,
  • HE Yongjin ,
  • CHEN Bilian
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  • 1(College of Life Sciences, Fujian Normal University, Fuzhou 350117, China)
    2(Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350117, China)

Received date: 2023-04-08

  Revised date: 2023-04-23

  Online published: 2023-09-27

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Abstract

In this study, a new extraction method mediated by organic solvent and salt was developed to simultaneously extract phycocyanin and lipids from the unbroken Spirulina platensis. To obtain the maximum co-production extraction performance, the process parameters of the developed extraction method with n-hexane and calcium chloride were optimized. After optimization, the optimal conditions to extract phycocyanin and oil from unbroken Spirulina platensis using the n-hexane and calcium chloride-mediated method were that calcium chloride concentration was 3%, the volume ratio of n-hexane to suspension was 2:1, the ratio of algal biomass to liquid was 1:60 (g:mL), and extraction time was 3 h. Under the optimized conditions, the maximum extraction rates of phycocyanin and oil by the exploited extraction method were 71.06% and 26.19%, respectively. Based on these obtained results, the novel developed extraction method not only enables the co-production extraction of phycocyanin and lipids from Spirulina platensis but also has the function to separate the extracted phycocyanin and lipids, which provides important practical details to extract the desired bioactive substances from Spirulina platensis through Spirulina-derived deep processing.

Key words： Spirulina biomass; phycocyanin; lipid; extraction; separation

Cite this article

GUO Xu , WEI Dengxiao , ZHONG Cairong , LAN Ying , HE Yongjin , CHEN Bilian . A novel extraction method mediated with n-hexane and calcium chloride to concurrently extract phycocyanin and lipids from unbroken Spirulina platensis[J]. Food and Fermentation Industries, 2023 , 49(17) : 202 -208 . DOI: 10.13995/j.cnki.11-1802/ts.035762

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