In this work, the effects of amino acids and vitamins on the growth and lactic acid synthetic metabolism with Lactobacillus paracasei were investigated based on the original chemical defined medium for lactic acid production. Six amino acids and three vitamins which had obvious effects on cell growth and lactic acid production were determined by single component concentration gradient experiments, and they were used for Plackett-Burman experiment getting the result that biotin, aspartic acid and glutamic acid had the most noticeable effects on lactic acid production. Then the range of the three factors that obtained maximum responses value were determined through the steepest experiment, and finally their optimal concentrations were obtained by Box-Behnken experiment. The lactic acid fermentations in shake flask and 2.5 L bioreactor were carried out to verify the above results, showing all the parameters, lactic acid production, productivity and the conversion ratio of lactic acid to glucose, had significant increase compared to the original chemical defined medium. This work paved the way for the quantitative metabolic research of the strain.
SHI Shengchao
,
LIU Zhihao
,
WANG Yonghong
. Optimization of amino acids and vitamins components in complete chemical defined medium of Lactobacillus paracasei[J]. Food and Fermentation Industries, 2023
, 49(18)
: 24
-30
.
DOI: 10.13995/j.cnki.11-1802/ts.034380
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