Food and Fermentation Industries >

2023 , Vol. 49 >Issue 18: 140 - 147

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034267

Establishment and optimization of the ultrasonic-enzyme assisted aqueous two-phase extraction for obtaining the flavanones from Zhiqiao (Fructus aurantii)

  • CHEN Shanshan ,
  • HAN Leng ,
  • CHENG Yujiao ,
  • LI Guijie ,
  • FENG Ruizhang ,
  • LIU Wenwen ,
  • SUN Zhigao
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  • 1(Citrus Research Institute, Southwest University, Chongqing 400712, China)
    2(National Citrus Engineering Research Center, Chongqing 400712, China)
    3(Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education, Yibin University, Yibin 644000, China)

Received date: 2022-11-10

  Revised date: 2022-11-20

  Online published: 2023-10-25

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Abstract

Zhiqiao (Fructus aurantii) is a common medicinal and edible plant of citrus species in China. It contains abundant flavanones, namely neohesperidin and naringin, which are the major pharmacological components. To improve the extraction and separation efficiency of neohesperidin and naringin from Zhiqiao, the optimal conditions of the ultrasonic-assisted aqueous two-phase extraction using response surface methodology were explored, and a further ultrasonic-enzyme assisted aqueous two-phase extraction (UEA-ATPE) technique and a preparative liquid chromatography were established. The results showed that the optimal conditions of UEA-ATPE to obtain flavanones from Zhiqiao were as follows: mass fraction of ethanol, 27%; mass fraction of (NH4)2SO4, 21%; ultrasonic power, 137 W; the ratio of neutral protease:pectinase=1∶1 (mass ratio) in the complex enzymes. The yield of neohesperidin and naringin reached (92.27±2.13), (72.24±1.56) mg/g, respectively, which was better than that of water decoction, extraction using 50% ethanol, ultrasonic-assisted extraction using 95% ethanol and ultrasonic-assisted aqueous two-phase extraction, while the composition and content of unwanted extracts by the new method were less. The extracts were finally separated and purified using a preparative liquid chromatography and the purity of neohesperidin was 99.37%. The method has the advantages of high extraction efficiency and greenness, which is helpful for the deep development and utilization of Zhiqiao as a health food and improving its economic added value.

Key words： Zhiqiao (Fructus aurantii); flavanone; ultrasonic-enzyme assisted extraction; aqueous two-phase extraction; response surface method; neohesperidin

Cite this article

CHEN Shanshan , HAN Leng , CHENG Yujiao , LI Guijie , FENG Ruizhang , LIU Wenwen , SUN Zhigao . Establishment and optimization of the ultrasonic-enzyme assisted aqueous two-phase extraction for obtaining the flavanones from Zhiqiao (Fructus aurantii)[J]. Food and Fermentation Industries, 2023 , 49(18) : 140 -147 . DOI: 10.13995/j.cnki.11-1802/ts.034267

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