To study the effects of germination on the phenolic content and antioxidant capacity of wheat seedlings, the changes of seedling growth, total phenolic content, and antioxidant capacity of five varieties of wheat seedlings, were investigated at different germination stages. The changes in the activities of phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and 4-coumaric acid CoA ligase (4CL) were also investigated at different stages of phenolic synthesis. Results showed that germination significantly improved the activity of key enzymes for the synthesis of phenolics in wheat seedlings. The contents of total phenolics in wheat seedlings of 5 varieties increased to different degrees, respectively, and the difference in total phenolics in wheat seedlings of different varieties was significant. The phenolics content of Qiule 168 was the highest in 5 cultivars at 6 days of germination, which was 740.72 mg GAE/100 g DW. There were great differences in the growth status of wheat seedlings of different varieties. ABTS cationic radical and DPPH radical scavenging activities of wheat seedlings also increased significantly with the extension of germination time. Correlation analysis showed that there was a significant correlation between total phenolic content and antioxidant capacity (P<0.05). In conclusion, germination could activate the key enzymes of phenolic synthesis in wheat seedlings, significantly improving the phenolic content and antioxidant capacity of wheat seedlings, and the accumulation of phenolic substances was an important condition to improve the antioxidant capacity. The research results could provide a theoretical basis for developing wheat seedlings into foods rich in functional components such as phenolics.
MA Yan
,
WANG Jing
,
CHENG Yongxia
,
QIAO Mingwu
,
SONG Lianjun
,
HUANG Xianqing
,
LIU Xingkai
,
QU Lingyu
,
WANG Shiming
. Effects of germination on phenolics and antioxidant capacity of wheat seedlings[J]. Food and Fermentation Industries, 2023
, 49(18)
: 178
-185
.
DOI: 10.13995/j.cnki.11-1802/ts.033123
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