Effects of postharvest phenylalanine treatment on peel coloration of ‘Hujingmilu’ peach

  • MA Lijie ,
  • SHAO Xiaoda ,
  • ZHAO Sheng ,
  • PAN Hong ,
  • ZHAO Jing ,
  • ZHANG Yingtong ,
  • LING Jun ,
  • LI Pengxia ,
  • HUANG Wen ,
  • ZHOU Hongsheng
Expand
  • 1(College of Food Science, Shenyang Agricultural University, Shenyang 110866, China)
    2(Kunshan Yiqun Agricultural Products Company limited, Suzhou 215300, China)
    3(Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cold Chain Logistics Technology for Agro-product, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China)
    4(Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China)
    5(Nanjing Institute of Vegetable Science, Nanjing 210042, China)

Received date: 2022-07-07

  Revised date: 2022-08-05

  Online published: 2023-10-25

Abstract

To explore the effect of phenylalanine treatment on the peel color of peach during storage, the bagged ‘Hujingmilu’ peaches were soaked with 8 mmol/L phenylalanine for 10 min and then stored at room temperature (22±2) ℃ for 8 days, the effects of phenylalanine treatment on the fruit appearance, color difference, anthocyanin content, and gene expression levels of the anthocyanin metabolic pathway-related genes of the peach were studied in this research. Results showed that post-harvest application of phenylalanine could significantly induce the red color, improve the ΔE value, a* value, color index of red grapes (CIRG) value, and total anthocyanin content of postharvest peach peel, compared with the control group. On the 8th day, the content of anthocyanin in the phenylalanine treatment group was 1.64 times more than the control group. Phenylalanine treatment significantly promoted the expression of anthocyanin synthesis-related structural genes (PAL, F3H, DFR, UFGT) and regulatory genes (MYB10.1, bHLH3, WD40-1) in peach peel at the early stage of storage. This paper confirmed that phenylalanine treatment could effectively enhance the biosynthesis of anthocyanin and improve the peach peel color development of the bagged peach. This research provides technical guidance and theoretical reference for the regulation of peach color by using phenylalanine during postharvest storage.

Cite this article

MA Lijie , SHAO Xiaoda , ZHAO Sheng , PAN Hong , ZHAO Jing , ZHANG Yingtong , LING Jun , LI Pengxia , HUANG Wen , ZHOU Hongsheng . Effects of postharvest phenylalanine treatment on peel coloration of ‘Hujingmilu’ peach[J]. Food and Fermentation Industries, 2023 , 49(18) : 195 -201 . DOI: 10.13995/j.cnki.11-1802/ts.032905

References

[1] 田梦瑶, 周宏胜, 唐婷婷, 等.外源蔗糖处理对采后桃果皮色泽形成的影响[J].食品科学, 2022, 43(1):177-183.
TIAN M Y, ZHOU H S, TANG T T, et al.Effect of exogenous sucrose treatment on the peel coloration in postharvest peaches[J].Food Science, 2022, 43(1):177-183.
[2] RAHIM M A, BUSATTO N, TRAINOTTI L.Regulation of anthocyanin biosynthesis in peach fruits[J].Planta, 2014, 240(5):913-929.
[3] 徐僡, 郑远静, 高方平, 等.花色苷的生物合成及其影响因素研究进展[J].江苏农业学报, 2019, 35(5):1246-1253.
XU H, ZHENG Y J, GAO F P, et al.Advances in the biosynthesis and influencing factors of anthocyanins[J].Jiangsu Journal of Agricultural Sciences, 2019, 35(5):1246-1253.
[4] ZENG X Q, LI H, JIANG W B, et al.Phytochemical compositions, health-promoting properties and food applications of crabapples:A review[J].Food Chemistry, 2022, 386:132789.
[5] 何平, 李林光, 王海波, 等.套袋对‘秋雪’桃果实品质及花青素合成相关基因表达的影响[J].植物生理学报, 2018, 54(2):273-281.
HE P, LI L G, WANG H B, et al.Effect of bagging on fruit quality and anthocyanin synthesis-related gene expression of ‘Qiuxue’ peach[J].Plant Physiology Journal, 2018, 54(2):273-281.
[6] 庄维兵, 刘天宇, 束晓春, 等.植物体内花青素苷生物合成及呈色的分子调控机制[J].植物生理学报, 2018, 54(11):1630-1644.
ZHUANG W B, LIU T Y, SHU X C, et al.The molecular regulation mechanism of anthocyanin biosynthesis and coloration in plants[J].Plant Physiology Journal, 2018, 54(11):1630-1644.
[7] 戴思兰, 洪艳.基于花青素苷合成和呈色机理的观赏植物花色改良分子育种[J].中国农业科学, 2016, 49(3):529-542.
DAI S L, HONG Y.Molecular breeding for flower colors modification on ornamental plants based on the mechanism of anthocyanins biosynthesis and coloration[J].Scientia Agricultura Sinica, 2016, 49(3):529-542.
[8] GAO H N, JIANG H, CUI J Y, et al.Review:The effects of hormones and environmental factors on anthocyanin biosynthesis in apple[J].Plant Science, 2021, 312:111024.
[9] KUMAR PATEL M, MAURER D, FEYGENBERG O, et al.Phenylalanine:A promising inducer of fruit resistance to postharvest pathogens[J].Foods, 2020, 9(5):646.
[10] PERKOWSKI M C, WARPEHA K M.Phenylalanine roles in the seed-to-seedling stage:Not just an amino acid[J].Plant Science, 2019, 289(C):110223.
[11] OLIVA M, HATAN E, KUMAR V, et al.Increased phenylalanine levels in plant leaves reduces susceptibility to Botrytis cinerea[J].Plant Science, 2020, 290:110289.
[12] AGHDAM M S, MORADI M, RAZAVI F, et al.Exogenous phenylalanine application promotes chilling tolerance in tomato fruits during cold storage by ensuring supply of NADPH for activation of ROS scavenging systems[J].Scientia Horticulturae, 2019, 246:818-825.
[13] SAIDI L, DUANIS-ASSAF D, GALSARKER O, et al.Elicitation of fruit defense response by active edible coatings embedded with phenylalanine to improve quality and storability of avocado fruit[J].Postharvest Biology and Technology, 2021, 174:111442.
[14] CHEN Q B, MAN C, LI D N, et al.Arogenate dehydratase isoforms differentially regulate anthocyanin biosynthesis in Arabidopsis thaliana[J].Molecular Plant, 2016, 9(12):1609-1619.
[15] PORTU J, LÓPEZ-ALFARO I, GÓMEZ-ALONSO S, et al.Changes on grape phenolic composition induced by grapevine foliar applications of phenylalanine and urea[J].Food Chemistry, 2015, 180:171-180.
[16] SOGVAR O B, RABIEI V, RAZAVI F, et al.Phenylalanine alleviates postharvest chilling injury of plum fruit by modulating antioxidant system and enhancing the accumulation of phenolic compound[J].Food Technology and Biotechnology, 2020, 58(4):433-444.
[17] 洪敏, 贺明阳, 王日葵, 等.塔罗科血橙室温贮藏期间花色苷和糖酸积累变化及相关代谢基因表达特征[J].浙江大学学报(农业与生命科学版), 2021, 47 (5):589-597.
HONG M, HE M Y, WANG R K, et al.Changes of anthocyanin, sugar and acid accumulation and expression characteristics of related metabolic genes in Tarocco blood oranges during room temperature storage[J].Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(5):589-597.
[18] ZHAO Y, DONG W Q, WANG K, et al.Differential sensitivity of fruit pigmentation to ultraviolet light between two peach cultivars[J].Frontiers in Plant Science, 2017, 8:1552.
[19] TANG T T, ZHOU H S, WANG L B, et al.Post-harvest application of methyl jasmonate or prohydrojasmon affects color development and anthocyanins biosynthesis in peach by regulation of sucrose metabolism[J].Frontiers in Nutrition, 2022, 9:871467.
[20] PATHARE P B, OPARA U L, AL-SAID F A.Colour measurement and analysis in fresh and processed foods:A review[J].Food and Bioprocess Technology, 2013, 6(1):36-60.
[21] CARREÑO J, MARTÍNEZ A, ALMELA L, et al.Measuring the color of table grapes[J].Color Research and Application, 1996, 21(1):50-54.
[22] XU Y H, LI S E, HUAN C, et al. Effects of 1-methylcyclopropene treatment on quality and anthocyanin biosynthesis in plum (Prunus salicina cv. Taoxingli) fruit during storage at a non-chilling temperature[J]. Postharvest Biology and Technology, 2020, 169: 111291.
[23] ZHAO Y, DONG W Q, ZHU Y C, et al.PpGST1, an anthocyanin-related glutathione S-transferase gene, is essential for fruit coloration in peach[J].Plant Biotechnology Journal, 2020, 18 (5):1284-1295.
[24] LIU Y F, MA K X, QI Y W, et al.Transcriptional regulation of anthocyanin synthesis by MYB-bHLH-WDR complexes in kiwifruit (Actinidia chinensis)[J].Journal of Agricultural and Food Chemistry, 2021, 69(12):3677-3691.
[25] EDAHIRO J I, NAKAMURA M, SEKI M, et al.Enhanced accumulation of anthocyanin in cultured strawberry cells by repetitive feeding of L-phenylalanine into the medium[J].Journal of Bioscience and Bioengineering, 2005, 99(1):43-47.
[26] PORTU J, LÓPEZ R, SANTAMARÍA P, et al.Elicitation with methyl jasmonate supported by precursor feeding with phenylalanine:Effect on Garnacha grape phenolic content[J].Food Chemistry, 2017, 237:416-422.
[27] ZHANG Y T, LING J, ZHOU H S, et al.1-Methylcyclopropene counteracts ethylene inhibition of anthocyanin accumulation in peach skin after harvest[J].Postharvest Biology and Technology, 2022, 183:111737.
[28] BISWAS T, MATHUR A, GUPTA V, et al.Elicitation and phenylalanine precursor feeding based modulation of in vitro anthocyanin production, enzyme activity and gene expression in an Indian ginseng congener- Panax sikkimensis Ban[J].Industrial Crops & Products, 2020, 145:111986.
[29] FANYUK M, KUMAR PATEL M, OVADIA R, et al.Preharvest application of phenylalanine induces red color in mango and apple fruit’s skin[J].Antioxidants, 2022, 11(3):491.
[30] GOHARI G, ZAREEI E, KULAK M, et al.Improving the berry quality and antioxidant potential of flame seedless grapes by foliar application of chitosan-phenylalanine nanocomposites (CS-Phe NCs)[J].Nanomaterials, 2021, 11(9):2287.
Outlines

/