Glucosamine is an important functional monosaccharide, which has large market demand. At present, the main industrial production method of glucosamine is acid hydrolysis using shells of shrimps and crabs, but this method can cause environmental pollution, while the single-microorganism fermentation method cannot accumulate a large amount of glucosamine due to the influence of intermediate metabolite. In this study, the synthesis of glucosamine was divided into two steps: efficient synthesis of N-acetyl glucosamine and its deacetylation via division of labor of Escherichia coli co-cultures. Endogenous and exogenous deacetylase encoding genes were respectively enhanced and compared, and further optimization of inoculation ratio of two strains and fermentation conditions were conducted to improve the conversion efficiency from N-acetyl glucosamine to glucosamine. The final co-culture system produced 11.21 g/L glucosamine at 24 h in shake flask, which was about 3.1 times higher than the initial control system. This strategy can be used as a reference for the synthesis of other products which cannot be highly accumulated due to the feedback inhibition of key enzymes from intermediate metabolites.
ZHAO Kexin
,
GENG Zihao
,
YI Jinhang
,
ZHUO Mingyang
,
ZHANG Chunyue
,
SUN Wenchao
,
MA Qian
. Fermentative production of glucosamine using Escherichia coli co-cultures via division of labor[J]. Food and Fermentation Industries, 2023
, 49(19)
: 60
-66
.
DOI: 10.13995/j.cnki.11-1802/ts.034517
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