Food and Fermentation Industries >

2023 , Vol. 49 >Issue 19: 105 - 111

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034153

Breeding of very high gravity brewing strains based on droplet microfluidic technology

  • HE Xiuli ,
  • SHI Yamin ,
  • XIE Xin ,
  • GUO Liyun ,
  • FU Xiaofen
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  • (Technology Center of Beijing Yanjing Beer Co. Ltd., Beijing Key Laboratory of Beer Brewing Technology, Beijing 101300, China)

Received date: 2022-10-30

  Revised date: 2022-12-15

  Online published: 2023-11-01

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Abstract

While the ultra-high gravity brewing technology reduces costs and energy consumption, good stress-resistant performance is a must for yeast needed in high gravity brewing. To get ultra-high gravity brewing strain of reliable capability through a more efficient screening process, this study hereby establishes a high-throughput screening method for brewer's yeast based on droplet microfluidic technology. The Saccharomyces pastorianus 02 was used as the starting yeast strain, with atmospheric room temperature plasma (ARTP) mutagenesis, droplet microfluidic initial screening, α-glycosidase (α-GC) and alcohol dehydrogenase Ⅱ (ADH Ⅱ) activity re-screening, and fermentation experiment, results in a very high gravity brewing strain 9-50 of good genetic and fermentation stability. Compared with the starting strain, the resulting strain proliferated vigorously with a fermentation period shortened by 42 h in the pilot scale, and adapted to an environment of 9% vol high ethanol concentration while delivering a fermentation degree of more than 66%, and diacetyl content of 0.06 mg/L with well-balanced aroma of alcohol ester, brining it good application prospects in the brewing industry.

Key words： yeast; droplet microfluidic; ARTP mutagenesis; high-throughput screening; high gravity brewing

Cite this article

HE Xiuli , SHI Yamin , XIE Xin , GUO Liyun , FU Xiaofen . Breeding of very high gravity brewing strains based on droplet microfluidic technology[J]. Food and Fermentation Industries, 2023 , 49(19) : 105 -111 . DOI: 10.13995/j.cnki.11-1802/ts.034153

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