Food and Fermentation Industries >

2023 , Vol. 49 >Issue 19: 190 - 198

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033018

The phytochemical profiling, antioxidant capacity, α-glucosidase and α-amylase inhibition abilities of different parts of Paeonia suffruticosa ‘Hai Huang'

  • DAI Manting ,
  • SONG Jing ,
  • YU Xiao ,
  • ZHANG Pingfang ,
  • PING Huailei ,
  • LIU Zirong ,
  • WANG Juan ,
  • WANG Zhenxing
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  • 1(College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China)
    2(College of Forestry, Southwest Forestry University, Kunming 650224, China)
    3(Eco-development Academy, Southwest Forestry University, Kunming 650224, China)
    4(College of Life Sciences, Southwest Forestry University, Kunming 650224, China)

Received date: 2022-07-18

  Revised date: 2022-08-19

  Online published: 2023-11-01

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Abstract

To compare the phytochemical profiling and functional activity of petals, calyx, pistil, and stamen of Paeonia suffruticosa ‘Hai Huang'. The total phenolic (TPC) and total flavonoid (TFC) contents of these four parts were quantified, and their 5 polyphenols contents were further analyzed by HPLC. Finally, the antioxidant ability, α-glucosidase and α-amylase inhibition abilities of these four parts were evaluated. Results showed that the TPC of these four parts ranged between 347.72 to 917.82 mg GAE/g, while TFC ranged between 13.21 to 58.71 mg RT/g. Of these, pistil and calyx showed significantly higher TPC and TFC than those of the other two parts (P<0.05). From HPLC results, remarkable differences in their bioactive compound contents were observed between different parts of the samples (P<0.05). Among them, the petal detected the most compounds frequently, and the stamen detected the highest compounds contents. In addition, the calyx displayed the strongest DPPH radical and ABTS radical with IC50 values of 44.97 and 74.44 μg/mL, respectively. Pistil had the best ferric capacity, and its value was 691.46 mg FeSO4/g. In these four parts, the petal showed strong inhibition of α-glucosidase, while stamen displayed a superior inhibitory effect against α-amylase, which was better than the positive control acarbose. In summary, the pistil and calyx parts of Hai Huang exhibited superior antioxidant activity, while petal and stamen showed certain in vitro hypoglycemic activity. This study can provide some scientific basis for developing Hai Huang into natural antioxidant and hypoglycemic.

Key words： Paeonia suffruticosa ‘Hai Huang'; bioactive constituents; antioxidant capacity; α-glucosidase; α-amylase

Cite this article

DAI Manting , SONG Jing , YU Xiao , ZHANG Pingfang , PING Huailei , LIU Zirong , WANG Juan , WANG Zhenxing . The phytochemical profiling, antioxidant capacity, α-glucosidase and α-amylase inhibition abilities of different parts of Paeonia suffruticosa ‘Hai Huang'[J]. Food and Fermentation Industries, 2023 , 49(19) : 190 -198 . DOI: 10.13995/j.cnki.11-1802/ts.033018

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