Food and Fermentation Industries >

2023 , Vol. 49 >Issue 20: 38 - 44

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034406

Effects of six Chinese herbal extracts on ergosterol in submerged fermentation of Morchella esculenta

  • GUO Gengxin ,
  • YUN Jianmin ,
  • WANG Biao ,
  • ZHAO Yuxuan ,
  • LI Wenhui ,
  • QU Yuling
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2022-11-23

  Revised date: 2023-01-11

  Online published: 2023-11-20

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Abstract

The effects of six authentic Chinese medical herb extracts from Gansu on the biomass and ergosterol yield of Morchella esculenta by submerged fermentation were studied. The single factor test combined with response surface methodology (RSM) was used to screen the Chinese herbal medicine suitable for the fermentation of M. esculenta to produce ergosterol. Meanwhile, the effects of adding Hedysarum polybotrys extract on ergosterol in submerged fermentation of M. esculenta was investigated, and the fermentation parameters were optimized. Finally, the fermentation system of M. esculenta in the liquid environment of Chinese herbal medicine was established. The results showed that Codonopsis pilosula, H. polybotrys, and Bupleurum umbelliferae could significantly promote the biomass and ergosterol yield of M. esculenta, among them, H. polybotrys had the best effect, the biomass dry weight and ergosterol yield was the largest at the dosage of 100 g/L, reaching 10.57 g/L and 47.69 mg/L, it was 2.83 times and 2.50 times of the control group, respectively; Glycyrrhiza uralensis, Angelica sinensis, and Isatis indigotica could all promote the growth of M. esculenta mycelia to a certain extent at the dosage of 50 g/L, while exceeding 50 g/L, it had strong inhibitory effect to mycelia growth. RSM results showed that the order of factors affecting ergosterol content was: fermentation time>dosage of H. polybotrys>fermentation temperature; the optimum technological parameters were as followed: dosage of H. polybotrys 97.60 g/L, 26 ℃, fermented for 11 d, inoculation volume 8%, shaking speed 180 r/min, under these conditions, the ergosterol yield reached 59.16 mg/L, which was 21.95% higher than that before optimization. The results can provide theoretical basis and reference for related industries to promote the liquid fermentation of ergosterol by M. esculenta using Chinese herbal extracts.

Key words： Chinese herbal medicine; Morchella esculenta; submerged fermentation; ergosterol; response surface methodology

Cite this article

GUO Gengxin , YUN Jianmin , WANG Biao , ZHAO Yuxuan , LI Wenhui , QU Yuling . Effects of six Chinese herbal extracts on ergosterol in submerged fermentation of Morchella esculenta[J]. Food and Fermentation Industries, 2023 , 49(20) : 38 -44 . DOI: 10.13995/j.cnki.11-1802/ts.034406

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