Food and Fermentation Industries >

2023 , Vol. 49 >Issue 21: 89 - 94

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035742

Applicability studies of COⅠ and 16S rRNA genes in the identification of fish maw species

  • GUAN Jinmeng ,
  • MAO Xiangzhao ,
  • MA Haixia ,
  • DENG Jianchao ,
  • HU Xiao ,
  • QI Bo ,
  • YANG Xianqing
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  • 1(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
    2(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China)

Received date: 2023-04-06

  Revised date: 2023-05-03

  Online published: 2023-12-08

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Abstract

To establish an accurate identification method for fish maw, this study analyzed the applicability of the cytochrome c oxidase subunit Ⅰ (COⅠ) gene and 16S ribosomal RNA (16S rRNA) gene from a DNA barcoding perspective in fish maw. Evolutionary analysis was performed by amplifying and sequencing COⅠ and 16S rRNA gene fragments of 30 samples from five major types of fish maw products, using three pairs of universal primers. DNA sequence and genetic difference analyses were conducted using DnaSP 6.12 and Mega 11, then an evolutionary tree was constructed based on the Neighbor-Joining method. The results revealed that both gene fragments displayed a nucleotide base bias, with the sequence variation rate of the 16S rRNA gene significantly lower than that of the COⅠ gene, indicating greater genetic stability for the 16S rRNA gene. The COⅠ and 16S rRNA sequences exhibited average intra-specific genetic distances of 0.56% and 0.37%, respectively; and average inter-specific genetic distances of 20.26% and 13.93%, respectively. Phylogenetic analysis revealed that both genes can differentiate among the five major classes of fish maw, with the 16S rRNA gene exhibiting slightly lower discriminatory power than the COⅠ gene in identifying certain species with high homology. Consequently, it is recommended that COⅠ and 16S rRNA genes be used together as a DNA barcode for fish maw species identification, resulting in 100% identification accuracy.

Key words： fish maw; DNA barcoding; COⅠ gene; 16S rRNA gene; species identification; phylogenetic analysis

Cite this article

GUAN Jinmeng , MAO Xiangzhao , MA Haixia , DENG Jianchao , HU Xiao , QI Bo , YANG Xianqing . Applicability studies of COⅠ and 16S rRNA genes in the identification of fish maw species[J]. Food and Fermentation Industries, 2023 , 49(21) : 89 -94 . DOI: 10.13995/j.cnki.11-1802/ts.035742

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