Food and Fermentation Industries >

2023 , Vol. 49 >Issue 22: 19 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034949

Screening of bacteriocin-producing lactic acid bacteria and the probiotic properties evaluation

  • ZHANG Le ,
  • DING Yizhen ,
  • PAN Yuanna ,
  • LI Ke ,
  • GENG Yan ,
  • ZHOU Juan ,
  • XU Hongyu ,
  • LI Heng ,
  • XU Zhenghong ,
  • SHI Jinsong
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  • 1(College of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Research Center for Food Fermentation and Food Biomanufacturing, Wuxi 214122, China)

Received date: 2023-01-27

  Revised date: 2023-03-06

  Online published: 2023-12-25

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Abstract

Bacteriocin-producing lactic acid bacteria exhibit probiotic effects through regulating intestinal microecology. In the present study, a bacteriocin-producing strain of Lactobacillus harbinensis P1-1 with strong antibacterial ability was screened from 20 strains of lactic acid bacteria by eliminating the bacteriostasis effects of organic acid and hydrogen peroxide. The probiotic effects of L. harbinensis P1-1 were evaluated and the relationship between this strain and some other microorganisms were evaluated through microbial coculture. The results showed that the survival rate of L. harbinensis P1-1 in artificial gastrointestinal fluids could reach more than 90%, while the survival rate in 1.2 g/L bile salt was 56.2%. It indicated that this strain possessed good gastrointestinal digestive fluid tolerance. L. harbinensis P1-1 inhibited the proliferation of Escherichia coli ATCC 25922 and Staphylococcus epidermidis ATCC 12228 significantly with the inhibition rate reached 86.3% and 89.5% at 24 h, respectively. The co-culture method was utilized to investigate the probiotic properties of L. harbinensis P1-1. It showed L. harbinensis P1-1 exerted little effects on growth of the two probiotic L. plantarum CGMCC No.18389 and Escherichia coli Nissle 1917, while the growth of two conditional pathogens were both inhibited apparently with a survival rate of less than 90%. Meanwhile, the antibacterial activity of L. harbinensis P1-1 was enhanced by co-cultured with all these microorganisms, which represented that L. harbinensis P1-1 possessed positive intestinal prebiotic activity and had the potential for further exploration of its performance and application.

Key words： lactic acid bacteria; bacteriocin; bacteriostasis; co-culture; Lactobacillus harbinensis

Cite this article

ZHANG Le , DING Yizhen , PAN Yuanna , LI Ke , GENG Yan , ZHOU Juan , XU Hongyu , LI Heng , XU Zhenghong , SHI Jinsong . Screening of bacteriocin-producing lactic acid bacteria and the probiotic properties evaluation[J]. Food and Fermentation Industries, 2023 , 49(22) : 19 -26 . DOI: 10.13995/j.cnki.11-1802/ts.034949

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