Food and Fermentation Industries >

2023 , Vol. 49 >Issue 22: 189 - 193

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034840

Preparation of quantitative strains of Lactobacillus rhamnosus for the detection of folic acid in infant formula milk powder

  • ZHANG Tao ,
  • ZHANG Rui ,
  • ZHANG Zilun ,
  • LI Xian ,
  • ZHU Yanbo ,
  • LIU Yumeng ,
  • ZHANG Yalun ,
  • ZHOU Wei
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  • (Hebei Food Inspection and Research Institute, Hebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Shijiazhuang 050200, China)

Received date: 2023-01-07

  Revised date: 2023-02-15

  Online published: 2023-12-25

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Abstract

A quantitative strain of Lactobacillus rhamnosus was prepared to optimize the determination of folic acid in infant formula milk powder by biological method. Make the suitable test conditions of the strain by adding the protecting agent to the freeze-stored strain, do the transport condition simulation test and storage condition simulation experiment to verify the stability of the bacteroids, randomly took 10 bottles of the strain to do the uniformity verification; L. rhamnosus quantitative strains were applied to test the folic acid content of quality control samples. The results showed that L. rhamnosus quantitative strains showed less variation in colony number at 25 ℃ and 37 ℃ conditions, the recovery was above 80% in both -20 ℃ and 4 ℃ environments, and L. rhamnosus quantitative strains were stable. One-way analysis of variance results indicated that the L. rhamnosus quantitative strain uniformity was good. The folic acid content of quality control samples were measured to be accurate. Quantitative strains of L. rhamnosus have the advantages of accurate test results, convenient preservation, and simplified experimental procedures, which meet the demand of folic acid detection in infant milk powder.

Key words： folic acid; Lactobacillus rhamnosus; quantitative strain; infant formula milk powder; homogeneity; stability

Cite this article

ZHANG Tao , ZHANG Rui , ZHANG Zilun , LI Xian , ZHU Yanbo , LIU Yumeng , ZHANG Yalun , ZHOU Wei . Preparation of quantitative strains of Lactobacillus rhamnosus for the detection of folic acid in infant formula milk powder[J]. Food and Fermentation Industries, 2023 , 49(22) : 189 -193 . DOI: 10.13995/j.cnki.11-1802/ts.034840

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