Food and Fermentation Industries >

2023 , Vol. 49 >Issue 23: 76 - 83

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035024

A preliminary study on the synthesis and related characteristics of 2-phenylethanol from a non-Saccharomyces cerevisiae strain

  • WANG Congjie ,
  • QIAN Yunkai ,
  • YAN Hejing ,
  • CUI Zongyan ,
  • SHI Wenqi ,
  • LUO Lisha ,
  • XIAO Yanxia ,
  • SHAN Chao ,
  • GE Chao
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  • 1(College of Food Science & Technology,Hebei Normal University of Science & Technology, Qinhuangdao 066004, China)
    2(Qinhuangdao Customs Technology Center, Qinhuangdao 066004, China)

Received date: 2023-02-10

  Revised date: 2023-04-14

  Online published: 2024-01-02

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Abstract

As an important quorum-sensing molecule in Saccharomyces cerevisiae and alcohols, which have important contributions to wine aroma, the synthesis and regulatory mechanism of 2-phenylethanol(2-PE) have been studied in S. cerevisiae, but less in non-Saccharomyces cerevisiae. It is difficult to understand the physiological and metabolic interactions between non-S. cerevisiae and S. cerevisiae induced by 2-PE in a mixed fermentation system and its impact on wine flavor. In this study, we screened a non-S. cerevisiae strain Mp-57, with dimorphic transformation using a restricted nitrogen source medium, and the 2-PE in the culture medium of Mp-57 strain was identified and detected by gas chromatography. The synthesis of 2-PE was also investigated. First, we found that the synthesis of 2-PE by Mp-57 strain depended on cell density. When the cell density reached 107 CFU/mL, 2-PE was detected in the culture medium, and the threshold cell density was the same as that of S. cerevisiae. Second, the synthesis rate of 2-PE showed a bell-shaped curve; that is, the synthesis rate increased with the extension of culture time at the beginning, reached the maximum value at the end of the logarithmic stage, and then rapidly decreased. As a result, the 2-PE content in the supernatant increased continuously from the beginning to the end of the quiescent period and remained at a high level. In contrast to previous studies, non-S. cerevisiae Mp-57 showed stronger 2-PE synthesis ability, which was significantly higher than that of S. cerevisiae Ds. Finally, the effects of 2-PE on the fermentation properties of Mp-57 and Ds were investigated, and it was found that 2-PE was similar to the signal molecule, which affected the growth of Mp-57 and Ds in a dose-dependent manner. It promoted yeast growth at a concentration of 50 μmol/L and inhibited yeast growth at a concentration of 500 μmol/L. Moreover, 2-PE at 50 μmol/L improved the tolerance of Mp-57 and Ds to ethanol and significantly promoted the formation of biofilm of strain Mp-57. The results provide a theoretical basis and experimental strains for studying the interaction mechanism between non-S. cerevisiae and S. cerevisiae in a mixed fermentation system.

Key words： nitrogen restriction; non-Saccharomyces cerevisiae; 2-phenylethanol; quorum sensing; biofilm; ethanol tolerance; dimorphic transformation

Cite this article

WANG Congjie , QIAN Yunkai , YAN Hejing , CUI Zongyan , SHI Wenqi , LUO Lisha , XIAO Yanxia , SHAN Chao , GE Chao . A preliminary study on the synthesis and related characteristics of 2-phenylethanol from a non-Saccharomyces cerevisiae strain[J]. Food and Fermentation Industries, 2023 , 49(23) : 76 -83 . DOI: 10.13995/j.cnki.11-1802/ts.035024

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