To analyze the characteristics of microbial flora of Zha-chili from Wushan and Fengjie County in Chongqing, high-throughput sequencing and isolation and identification-based methods were used. Based on the results of high-throughput sequencing, there were 11 dominant genera in Zha-chili from the two regions, including Lactobacillus, Levactobacter, Levilactobacillus, Companilactobacter, Weissella, and Prevotella, with the relative abundance of more than 5%. It is notable that there was a high relative abundance of Prevotella and Clostridium in Zha-chili samples from Wushan County. Based on linear discriminant analysis effect size (LEfSe) analysis, Prevotella, and Tatumella could be used as biomarker genera to distinguish Zha-chili in Wushan and Fengjie, respectively. Eighteen LAB strains were isolated from Zha-chili in Chongqing by isolation and identification-based methods, and they were identified as eight genera. Combining high-throughput sequencing and isolation and identification methods, the dominant genera of Levilactobacillus and Lentilactobacillus were identified as Levilactobacillus brevis and Lentilactobacillus buchneri, respectively. This research is expected to provide strain resources and data support for the industrialization development of Zha-chili.
ZHANG Zhendong
,
LI Xuefu
,
XIAO Qiuyang
,
ZHAN Jie
,
ZHAO Huijun
,
GUO Zhuang
,
WANG Yurong
. Microbial diversity of Zha-chili from Fengjie and Wushan County using high-throughput sequencing and isolation and identification-based methods[J]. Food and Fermentation Industries, 2023
, 49(24)
: 178
-185
.
DOI: 10.13995/j.cnki.11-1802/ts.034967
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