Aging is accompanied by the loss of hyaluronic acid (HA), which causes health problems such as skin aging, dazzling, joint pain, and memory decline. This study intended to explore the regulation of HA and anti-aging effects by oral Lactiplantibacillus plantarum CCFM1296 live bacteria and prepared postbiotics in vivo. The aging mouse model was induced by subcutaneous injection of 500 mg/(kg·d) D-galactose, and 5×109 CFU/kg Lactiplantibacillus plantarum CCFM1295, CCFM1296, CCFM1297 live bacteria (CCFM1295-L, CCFM1296-L, and CCFM1297-L) and 500 mg/kg dose of postbiotics prepared by L. plantarum CCFM1295, CCFM1296, CCFM1297(CCFM1295-D, CCFM1296-D, and CCFM1297-D) were administered daily for about 9 weeks. The content of HA in skin, joints, brain, and eyes of mice was measured and the mRNA expression of enzymes related to HA synthesis were quantified. In addition, aging-related serum biochemical indexes, skin moisture and elasticity, and skin antioxidant levels of mice were also detected. The results showed that both CCFM1296-L and CCFM1296-D promoted the content of HA in skin, joints, and eyes, and stimulated the expression level of HAS2 mRNA in tissues. CCFM1296-D also accelerate HA content in the brain and up-regulated the expression level of HAS2 mRNA. HAS2 is one of the key enzymes for HA biosynthesis, and may affect the level of HA. Further test found that CCFM1296-L and CCCFM1296-D increased CHE activity and decreased serum CRP, ALT, AST, ALP, and CK levels. CCFM1296-L can also increase skin moisture and skin elasticity, and improve the activity of CAT, GSH-Px and T-AOC in skin tissue to increase its defense ability. In general, L. plantarum CCFM1296 and prepared postbiotics can increase tissue HA level by up-regulating the expression level of HAS2 mRNA, thereby assisting the body to counteract aging.
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