Analysis of volatile compounds in different varieties of Brassica rapa L. by headspace-gas chromatography-ion mobility spectrometry

  • YUE Li ,
  • ZULIPIYA·Maimaiti ,
  • WANG Jiamin ,
  • YU Ming ,
  • MAO Hongyan ,
  • REYILAMU·Hailili
Expand
  • 1(Research Institute of Food Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China)
    2(Xinjiang Alnuri Agricultural Science and Technology Development Co.Ltd., Keping 843600, China)

Received date: 2024-02-23

  Revised date: 2024-04-27

  Online published: 2025-03-10

Abstract

To investigate the differences in flavor substances in different varieties of Brassica rapa L., different varieties (purple, yellow, and white) of Brassica rapa L.were selected for the identification and analysis of volatile compounds.Headspace-gas chromatography-ion mobility spectrometry was used to detect the volatile compounds in the samples and establish the fingerprints of the volatile compounds in Brassica rapa L.A total of 109 volatile compounds were detected in the Brassica rapa L.samples, and 80 species were identified, including aldehydes (12%-20%), esters (23%-27%), alcohols (10%-16%), ketones (5%-10%), acids (3%-7%), olefins (1%), sulphur ethers (2%-7%), and others (7%-15%).The content of esters was the highest in Brassica rapa L.volatile compounds, followed by aldehydes and alcohols.The composition and content of volatile compounds in different varieties of Brassica rapa L.differed significantly, with the least number of volatile compounds in purple Brassica rapa L.and the most in white Brassica rapa L.The results of principal component analysis and Euclidean distance showed that there were significant differences in the characteristic volatile compounds of the three varieties of Brassica rapa L., with octanal, acetic acid, nonanal, and (E,E)-2,4-heptadienal for the purple Brassica rapa L., camphene, ethyl 2-methylbutanoate, ethyl diphthongol, and (E)-2-hexen-1-ol for the white Brassica rapa L., and 5-methyl-2-thiophenecarboxaldehyde, isovaleryl butyl, diketonyl alcohol, butanone, and 2,3-pentanedione for the yellow Brassica rapa L.Combined with orthogonal partial least squares-discrimination analysis, this study established a model and screened out 32 different volatile compounds with variable importance in projection greater than 1, which were used as different volatile compounds to distinguish different varieties of Brassica rapa L.

Cite this article

YUE Li , ZULIPIYA·Maimaiti , WANG Jiamin , YU Ming , MAO Hongyan , REYILAMU·Hailili . Analysis of volatile compounds in different varieties of Brassica rapa L. by headspace-gas chromatography-ion mobility spectrometry[J]. Food and Fermentation Industries, 2025 , 51(4) : 309 -318 . DOI: 10.13995/j.cnki.11-1802/ts.038942

References

[1] KHAN S, WAQAS M, DING F H, et al.The influence of various biochars on the bioaccessibility and bioaccumulation of PAHs and potentially toxic elements to turnips (Brassica rapa L.)[J].Journal of Hazardous Materials, 2015, 300:243-253.
[2] FERNANDES F, VALENTÃO P, SOUSA C, et al.Chemical and antioxidative assessment of dietary turnip (Brassica rapa var.rapa L.)[J].Food Chemistry, 2007, 105(3):1003-1010.
[3] CAO Q S, WANG G, PENG Y.A critical review on phytochemical profile and biological effects of turnip (Brassica rapa L.)[J].Frontiers in Nutrition, 2021, 8:721733.
[4] JIANG H, DUAN W S, ZHAO Y C, et al.Development of a flavor fingerprint using HS-GC-IMS for volatile compounds from steamed potatoes of different varieties[J].Foods, 2023, 12(11):2252.
[5] TAVEIRA M, FERNANDES F, GUEDES DE PINHO P, et al.Evolution of Brassica rapa var.rapa L.volatile composition by HS-SPME and GC/IT-MS[J].Microchemical Journal, 2009, 93(2):140-146.
[6] 马国财, 李雅雯, 王丽君, 等.不同种质资源芜菁花朵香气成分的研究[J].中国酿造, 2017, 36(11):161-164.
MA G C, LI Y W, WANG L J, et al.Aroma components of Brassica rapa flowers from different germplasm resources[J].China Brewing, 2017, 36(11):161-164.
[7] 高琦, 韩昊廷, 李加恒, 等.基于主成分分析法综合评价四种干燥方式对芜菁脆片香气品质的影响[J].食品工业科技, 2018, 39(22):212-218;224.
GAO Q, HAN H T, LI J H, et al.Comprehensive evaluation of four drying methods on aroma quality of turnip chips based on principal component analysis[J].Science and Technology of Food Industry, 2018, 39(22):212-218;224.
[8] ZHOU Y X, WANG D D, DUAN H, et al.Detection and analysis of volatile flavor compounds in different varieties and origins of goji berries using HS-GC-IMS[J].LWT, 2023, 187:115322.
[9] 顾凡, 陶亮, 代佳和, 等.HS-GC-IMS结合化学计量学分析6种野生蜂蜜的挥发性风味成分[J].中国食品学报, 2023, 23(11):320-331.
GU F, TAO L, DAI J H, et al.Analysis of volatile flavor components of six wild honeys based on HS-GC-IMS combined with chemometrics[J].Journal of Chinese Institute of Food Science and Technology, 2023, 23(11):320-331.
[10] WANG S Q, CHEN H T, SUN B G.Recent progress in food flavor analysis using gas chromatography-ion mobility spectrometry (GC-IMS)[J].Food Chemistry, 2020, 315:126158.
[11] LIAO Y Q, DING Y X, WU Y R, et al.Analysis of volatile compounds and flavor fingerprint in hairtail (Trichiurus lepturus) during air-drying using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS)[J].Frontiers in Nutrition, 2023, 9:1088128.
[12] SONG Y J, GUO T, LIU S J, et al.Identification of Polygonati Rhizoma in three species and from different producing areas of each species using HS-GC-IMS[J].LWT, 2022, 172:114142.
[13] YAO L Y, SUN J Y, LIANG Y, et al.Volatile fingerprints of Torreya grandis hydrosols under different downstream processes using HS-GC-IMS and the enhanced stability and bioactivity of hydrosols by high pressure homogenization[J].Food Control, 2022, 139:109058.
[14] 高雪, 孙靖, 王俊平.不同品种小麦发酵面团及馒头挥发性物质的差异分析[J].食品安全质量检测学报, 2023, 14(14):10-18.
GAO X, SUN J, WANG J P.Analysis on the differences of volatile substances in different wheat fermented dough and steamed bread[J].Journal of Food Safety & Quality, 2023, 14(14):10-18.
[15] LI Q Q, YANG S P, ZHANG R, et al.Characterization of honey peach (Prunus persica (L.) Batsch) aroma variation and unraveling the potential aroma metabolism mechanism through proteomics analysis under abiotic stress[J].Food Chemistry, 2022, 386:132720.
[16] 曹长春, 石景艳, 谢建春, 等.温度对“半胱氨酸-木糖”模型体系初始美拉德中间体及挥发性风味物质形成的影响[J].食品与发酵工业, 2015, 41(11):64-69.
CAO C C, SHI J Y, XIE J C, et al.The effect of temperature on the formation of initial Maillard intermediates and volatile flavor compounds in cysteine/xylose model reaction system[J].Food and Fermentation Industries, 2015, 41(11):64-69.
[17] MARCINKOWSKA M A, JELEŃ H H.Role of sulfur compounds in vegetable and mushroom aroma[J].Molecules, 2022, 27(18):6116.
[18] BLANK I.Sensory Relevance of Volatile Organic Sulfur Compounds in Food[M].ACS Symposium Series.Washington,DC:American Chemical Society,2002.
[19] SÁNCHEZ-GARCÍA F, MIRZAYEVA A, ROLDÁN A, et al.Effect of different cooking methods on sea lettuce (Ulva rigida) volatile compounds and sensory properties[J].Journal of the Science of Food and Agriculture, 2021, 101(3):970-980.
[20] 周琦, 郑畅, 万楚筠, 等.不同种植区域双低品种浓香菜籽油的呈香特征[J].食品科学, 2023, 44(22):287-295.
ZHOU Q, ZHENG C, WAN C Y, et al.Aroma characteristics of double-low fragrant rapeseed oils from different planting areas[J].Food Science, 2023, 44(22):287-295.
[21] LI M Q, YANG R W, ZHANG H, et al.Development of a flavor fingerprint by HS-GC-IMS with PCA for volatile compounds of Tricholoma matsutake Singer[J].Food Chemistry, 2019, 290:32-39.
[22] SONG J X, CHEN Q Q, BI J F, et al.GC/MS coupled with MOS e-nose and flash GC e-nose for volatile characterization of Chinese jujubes as affected by different drying methods[J].Food Chemistry, 2020, 331:127201.
[23] ZHOU S Q, FENG D, ZHOU Y X, et al.HS-GC-IMS detection of volatile organic compounds in cistanche powders under different treatment methods[J].LWT, 2022, 165:113730.
Outlines

/