Abstract: The
acid soluble collagen (ASC) of skin of Yunnan bream was extracted, and the amino
acids composition, subunit composition, structural characteristics, thermal
stability and solubility of which were investigated. Research showed that ASC was
rich in Gly, Pro, Ala, low in Tyr, Met, Cys, and the hydroxylation rate of proline
was 35.47%; SDS-PAGE revealed that ASC was mainly composed of α1、α2 and β chains, which was in accordance with the structural features
of type I collagen; The maximum UV absorption wavelength was at 230 nm; XRD and
FTIR showed that the molecule of ASC was closely and regularly arranged and the
helical structure of which was maintained; DSC showed the thermal denaturation
temperature of ASC were 86.5 and 226.2℃; SEM showed that ASC was
uniformly distributed and had a multi-layered micro structure, which was
suitable to be used as carrier in food, medicine and cosmetics industry, and base
material in biomedicine; the solubility of ASC was relatively high under acidic
(pH<4) and low NaCl concentration (<4% (w/v)) conditions. This paper provided a theoretical basis
for the comprehensive utilization of skin of Yunnan bream.
2017, Vol. 43 
