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食品与发酵工业  2018, Vol. 44 Issue (9): 254-261    DOI: 10.13995/j.cnki.11-1802/ts.016217
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
固相萃取整体捕集剂-气相色谱-质谱联用仪结合电子鼻技术对中华绒螯蟹关键脂质热氧化体系的构建
付雪艳1, 吴娜2, 袁凯1, 王锡昌1*
1 (上海海洋大学 食品学院,上海,201306)
2(江西农业大学 食品科学与工程学院,江西 南昌,330045)
Study key lipids in Eriocheir sinensis by establishing a thermal oxidation system using MMSE-GC-MS combined electronic nose
FU Xue-yan1, WU Na2, YUAN Kai1, WANG Xi-chang1*
1 (College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China)
2 (College of Food Science & Engineering, Jiangxi Agriculture University, Nanchang 330045, China)
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摘要 以雌性中华绒螯蟹关键脂质为研究对象,基于挥发性成分分析,从时间、温度、水分、氧气4个热处理环境因素出发,搭建热氧化体外模型并进行条件优化,为脂质氧化降解特性的研究提供理论基础。采用新型吸附材料(mono trap)作为顶空固相萃取整体捕集剂,结合气相色谱-质谱仪(GC-MS)对雌蟹性腺和肝胰腺在热处理过程中的挥发性物质进行测定,优化热处理时间和温度;在此基础上采用电子鼻技术结合主成分和雷达图分析法分别对性腺和肝胰腺中的关键脂质,甘油三酯(triglyceride, TG)和脑磷脂(phosphatidyl ethanolamine, PE)的水分和氧气进行优化。结果显示,最优热氧化体系条件分别为,TG: 100 ℃, 25 min,水分6 μL,有氧条件;PE: 100 ℃,25 min,水分0 μL,有氧条件。
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付雪艳
吴娜
袁凯
王锡昌
关键词:  中华绒螯蟹  关键脂质  甘油三酯  脑磷脂  热氧化体系    
Abstract: The thermal oxidation system (conditions: temperature, time, water, oxygen) of key lipids in female Eriocheir sinensis was established based on volatile compound analysis. The establishment of vitro model can provide a reaction condition for the research of oxidation degradation characteristics of lipids. The new adsorption material-Mono Trap, combined with gas chromatography mass spectrometry (GC-MS) was used to extract volatile compounds during the heat treatment of female crab gonads and hepatopancreas. Furthermore, electronic nose combined with principle components analysis (PCA) and radar picture analysis was used to optimize water and oxygen of Triglyceride (TG) and Phosphatidyl ethanolamine (PE). The results indicated that the optimal condition of thermal oxidation for TG and PE were: TG: 100 ℃, 25 min, 6 μL water, aerobic conditions; PE: 100 ℃, 25 min, 0 μL water, aerobic conditions.
Key words:  Eriocheir sinensis    key lipids    triglyceride (TG)    phosphatidyl ethanolamine (PE)    thermal oxidation system
收稿日期:  2017-11-07                出版日期:  2018-09-25      发布日期:  2018-10-30      期的出版日期:  2018-09-25
基金资助: 国家自然科学基金(31471608); 上海市现代农业产业技术体系(沪农科产字(2017)第4号)
作者简介:  硕士研究生(王锡昌教授为通讯作者,E-mail:xcwang@shou.edu.cn)。
引用本文:    
付雪艳,吴娜,袁凯,等. 固相萃取整体捕集剂-气相色谱-质谱联用仪结合电子鼻技术对中华绒螯蟹关键脂质热氧化体系的构建[J]. 食品与发酵工业, 2018, 44(9): 254-261.
FU Xue-yan,WU Na,YUAN Kai,et al. Study key lipids in Eriocheir sinensis by establishing a thermal oxidation system using MMSE-GC-MS combined electronic nose[J]. Food and Fermentation Industries, 2018, 44(9): 254-261.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.016217  或          http://sf1970.cnif.cn/CN/Y2018/V44/I9/254
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