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食品与发酵工业  2018, Vol. 44 Issue (12): 66-72    DOI: 10.13995/j.cnki.11-1802/ts.016647
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
淡红侧耳子实体多糖的分离纯化及结构探析
田有秋,贾金霞,束旭,任晓婕,关月,谢红,许杨洁,王昱沣*
(南京农业大学 食品科学技术学院,江苏 南京,210095)
Purification and structure analysis of polysaccharides from Pleurotus djamor
TIAN You-qiu, JIA Jin-xia, SHU Xu, REN Xiao-jie, GUAN Yue,XIE Hong, XU Yang-jie, WANG Yu-feng*
(College of Food Science and Technology, Nanjing Agriculture University, Nanjing 210095, China)
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摘要 以淡红侧耳子实体为原料,采用水提醇沉法进行提取,大孔阴离子交换树脂D315脱色,Sevage法脱蛋白,得到淡红侧耳子实体粗多糖;利用DEAE-纤维素-52和Sephadex G-150对粗多糖进行纯化;采用高效凝胶过滤色谱法和柱前衍生高效液相色谱法进行分子质量测定和单糖组成分析;通过紫外光谱扫描、红外光谱扫描及刚果红实验对其结构进行初步测定;并对其理化性质进行研究。结果表明,经纯化后得到3种组分PDP-1-1、PDP-2-1和PDP-3-1;相对分子质量分别为12.9、10.6和2 753.7 kDa;PDP-1-1由甘露糖、葡萄糖、半乳糖、阿拉伯糖和岩藻糖5种单糖组成,PDP-2-1由甘露糖、葡萄糖、半乳糖、木糖、阿拉伯糖和岩藻糖6种单糖组成,PDP-3-1由甘露糖、葡萄糖、半乳糖和岩藻糖4种单糖组成;紫外扫描三组分均不含蛋白质和核酸,红外扫描均显示具有多糖特征吸收峰;PDP-1-1和PDP-3-1具有三股螺旋结构。
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田有秋
贾金霞
束旭
任晓婕
关月
谢红
许杨洁
王昱沣
关键词:  淡红侧耳  多糖  分离纯化  结构  理化性质    
Abstract: Polysaccharides were obtained from Pleurotus djamor by hot water extraction and alcohol precipitation. After removal of pigments and proteins by macroporous anion exchange resin D315 and Sevage method respectively, polysaccharides were purified by DEAE-cellulose-52 and Sephadex G-150. Molecular weight distribution and monosaccharide composition were analyzed by high performance gel filtration chromatography (HPGFC) and pre-column high performance liquid chromatography. The structures were preliminarily analyzed by ultraviolet, infrared spectrum and Congo red experiments. Their physicochemical properties were further determined. The results showed that three components were obtained with the molecular weights 12.9, 10.6, 2753.7 kDa respectively. PDP-1-1 was composed of mannose, glucose, galactose, Arabia sugar and fucose; PDP-2-1 was composed of mannose, glucose, galactose, xylose, Arabia sugar and fucose; PDP-3-1 was composed of mannose, glucose, galactose and fucose. Ultraviolet spectrum confirmed that the fractions were free of protein and nucleic acid and the infrared spectrum showed the characteristic absorption peak of polysaccharides. PDP-1-1 and PDP-3-1 had triple-helical structure.
Key words:  Pleurotus djamor    polysaccharides    purification    structure    physicochemical properties
收稿日期:  2017-02-27                出版日期:  2018-12-25      发布日期:  2019-01-22      期的出版日期:  2018-12-25
基金资助: 国家大学生创新性实验计划项目(201710307057);江苏省自然科学基金资助项目(BK20171379);江苏省科技项
作者简介:  硕士研究生(王昱沣副教授为通讯作者,E-mail:joywangyu@sina.com)。
引用本文:    
田有秋,贾金霞,束旭,等. 淡红侧耳子实体多糖的分离纯化及结构探析[J]. 食品与发酵工业, 2018, 44(12): 66-72.
TIAN You-qiu,JIA Jin-xia,SHU Xu,et al. Purification and structure analysis of polysaccharides from Pleurotus djamor[J]. Food and Fermentation Industries, 2018, 44(12): 66-72.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.016647  或          http://sf1970.cnif.cn/CN/Y2018/V44/I12/66
[1] 李传华,曲明清,曹晖,等. 中国食用菌普通名名录[J]. 食用菌学报, 2013, 20(3): 50-72.
[2] 迟全勃,施鹏飞,王伟青,等. 红平菇和平菇的营养比较与分析[J]. 北京农业职业学院学报, 2015, 29(3): 16-19.
[3] 胡庭俊,陈灵然,程富胜. 多糖生物学研究概况[J]. 兽药与饲料添加剂, 2005, 10(5):26-29.
[4] 王谦,杨立华,刘玉霞. 桃红侧耳液体培养及其多糖的抗氧化活性研究[J]. 食用菌, 2005(3): 10-11.
[5] PHISUT N, TEERARAT I, PHANTIPHA C. Proximate compositions, nonvolatile taste components and antioxidant capacities of some dried edible mushrooms collected from Thailand [J]. Food Measure, 2015, 9(3): 259-268.
[6] RODRIGUES D M F, FREITAS A C, ROCHA-SANTOS T A P, et al. Chemical composition and nutritive value of Pleurotus citrinopileatus var cornucopiae, P. eryngii, P. salmoneo stramineus, Pholiota nameko and Hericium erinaceus [J]. Journal of Food Science and Technology, 2015, 52(11): 6 927-6 939.
[7] 王金玺,包振伟,刘笑,等. 响应曲面法优化平菇多糖提取工艺[J]. 中国酿造, 2013, 32(4): 108-112.
[8] 竹文坤,贺新生. 红平菇发酵工艺条件研究[J]. 食用菌, 2010(2): 13-14.
[9] ZHANG Jian-Jun, MENG Guang-yuan, ZHANG Chen, et al. The antioxidative effects of acidic-, alkalic-, and enzymatic-extractable mycelium zinc polysaccharides by Pleurotus djamor on liver and kidney of streptozocin-induced diabetic mice [J]. BMC Complementary and Alternative Medicine, 2015, 15: 440.
[10] 田有秋,武奔月,谢广杰,等. 一种淡红侧耳多糖的制备工艺[J]. 食品科学技术学报, 2017, 5(35): 72-77.
[11] 陈杨琼,伍春莲,侯怡铃,等. 松乳菇多糖含量的苯酚硫酸法检测研究[J]. 西华师范大学学报:自然科学版, 2012, 33(2): 135-138.
[12] 杨红燕,严楠楠,姜艳红,等. 苦荞茶多糖的体外抗氧化活性及其分离纯化[J]. 食品与发酵工业, 2014, 40(12): 94-99.
[13] 戴军,朱松,汤坚. PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖的单糖组成[J]. 分析测试学报, 2007,26(2): 206-210.
[14] LI Chao, FU Xiong, HUANG Qiang. Ultrasonic extraction and structural identification of polysaccharides from Prunella vulgaris and its antioxidant and antiproliferative activities [J]. European Food Research and Technology, 2015, 240(1): 49-60.
[15] 王艾平,周丽明. 考马斯亮蓝法测定茶籽多糖中蛋白质含量条件的优化[J]. 河南农业科学, 2014, 43(3): 150-153.
[16] 方东军,赵润琴,张晓娟. 茯苓多糖的总糖含量及糖醛酸含量测定[J]. 中医药信息, 2011, 28(4): 42-44.
[17] 叶红. 马尾藻多糖的分离纯化、生物活性及结构分析[D]. 南京:南京农业大学, 2008.
[18] MOKNI G A, SILA A, MAKLOUF G I, et al. Structural, functional, and ACE inhibitory properties of water-soluble polysaccharides from chickpea flours[J]. International Journal of Biological Macromolecules, 2015, 75(4): 276-282.
[19] JEDDOU K B, CHAARI F, MAKTOUF S, et al. Structural, functional, and antioxidant properties of water-soluble polysaccharides from potatoes peels [J]. Food Chemistry, 2016, 205(15): 97-105.
[20] SATITMANWIWAT S, RATANAKHANOKCHAI K, LAOHAKUNJIT N, et al. Improved purity and immunostimulatory activity of β-(1→3)(1→6)-glucan from Pleurotus sajorcaju using cell wall-degrading enzymes [J]. Journal of Agricultural and Food Chemistry, 2012, 60(21): 5 423-5 430.
[21] 乔德亮. 三角帆蚌多糖提取、纯化、生物活性及其结构[D]. 南京:南京农业大学, 2009.
[22] MA Gao-xing, YANG Wen-jian, MARIGA A M, et al. Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue Carbohydrate Polymers [J]. 2014, 114: 297-305.
[23] YANG Bao, WANG Jin-shui, ZHAO Mou-ming, et al. Identification of polysaccharides from pericarp tissues of litchi (Litchi chinensis Sonn.) fruit in relation to their antioxidant activities [J]. Carbohydrate Research, 2006, 341(5): 634-638.
[24] JIA Xue-jing, DONG Li-hua, YANG Yong, et al. Preliminary structural characterization and antioxidant activities of polysaccharides extracted from Hawk tea (Litsea coreana var. lanuginosa) [J]. Carbohydrate Polymers, 2013, 95(1): 195-199.
[25] 张倩,张民,王超. 枸杞多糖分离纯化及结构研究[J]. 食品与发酵工业, 2014, 40(12): 41-47.
[26] 王慧力. 茶树花多糖提取条件的优化、纯化及其结构鉴定[D]. 南京:南京农业大学, 2014.
[27] DING Xiang, HOU Yi-ling, HOU Wan-ru. Structure feature and antitumor activity of a novel polysaccharide isolated from Lactarius deliciosus Gray [J]. Carbohydrate Polymers, 2012, 89(2): 397-402.
[28] 张灵芝. 鸡枞菌子实体多糖分离纯化与结构分析及抗肿瘤活性研究[D]. 广州:华南理工大学, 2012.
[29] JIA Xue-jing, ZHANG Chao, QIU Jian-feng. Purification, structural characterization and anticancer activity of the novel polysaccharides from Rhynchosia minima root [J]. Carbohydrate Polymers, 2015,132: 67-71.
[30] 夏朝红,戴奇,房韦,等. 几种多糖的红外光谱研究[J]. 武汉理工大学学报, 2007,29(1): 45-47.
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