Analysis of genetic relationship of Vitis vinifera resources by SSR markers
MA Wen-rui1, YAN Mi2, LIU Ye-wei3, JIANG Wei2, QUAN Li1, WANG Xue-wei1, WU Yun1*, XUE Jie2*
1(Department of Food Science and Pharmacy, Xinjiang Agriculture University, Urumqi 830052, China) 2(China National Research Institute of Food and Fermentation Industries, Beijing 10015, China) 3(China Agricultural University, Beijing 10083, China)
Abstract: 10 kinds of Vitis vinifera were used as raw material to analyze genetic relationship and diversity of Various types of vitis vinifera resources by simple sequence repeats (SSR) molecular marker technique. Twelve pairs of polymorphic primers were used for SSR amplification. 213 DNA fragments were generated and all of them were polymorphic bands. The genetic similarity coefficient of SSR amplification results were analyzed by NTSYSpc2.10e software and the genetic similarity coefficient was 0.71 to 0.85 with the average genetic similarity coefficient of 0.76. The clustering dendrogram constructed by unweighted pair-group method with arithmetic mean (UPGMA) method indicated that 10 Vitis materials could be divided into two major groups at the similarity coefficient of 0.73. This study showed that SSR molecular marker analyzed the genetic diversity of different kinds of Vitis vinifera from the molecular level and revealed the relationship between different materials. After alleles assignment,there were only 6 pairs of primers selected out for the construction of germplasms molecular ID. At the same time, high polymorphism, good repeatability of VrZAG79, VrZAG62, VVMD27, VVMD5 and VVS2 primers were selected to establish SSR sites of fluorescence spectra of different varieties and thus construct SSR fingerprint database of Vitis vinifera, which provided a reference for the detection and identification of authenticity and purity of grape varieties and wine.
马文瑞,严密,刘业伟,等. 酿酒葡萄品种遗传多样性的简单序列重复分析和分子身份证的建立[J]. 食品与发酵工业, 2018, 44(9): 232-238.
MA Wen-rui,YAN Mi,LIU Ye-wei,et al. Analysis of genetic relationship of Vitis vinifera resources by SSR markers[J]. Food and Fermentation Industries, 2018, 44(9): 232-238.