酿酒葡萄品种遗传多样性的简单序列重复分析和分子身份证的建立

doi:10.13995/j.cnki.11-1802/ts.016844

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摘要以10个红白酿酒葡萄品种为试材,采用简单序列重复 (simple sequence repeats, SSR)分子标记技术,对供试材料进行了遗传多样性分析及分子身份证的构建。根据SSR-PCR聚丙烯酰胺凝胶图谱显示,选用的12对SSR引物共扩增出 213 个片段,多态性百分率为 100%。利用 NTSYSpc2.10e 软件进行遗传相似性系数分析,所有品种间的遗传相似系数变化范围在0.71~0.85之间,平均遗传相似系数为0.76。通过非加权类平均法(unweighted pair-group method with arithmetic mean, UPGMA)进行聚类分析,在遗传相似系数 0.73处,将10个葡萄品种分成了2个类群,在一定程度上揭示了红白酿酒葡萄之间的亲缘关系。将扩增片段图谱按大小赋值后利用6对引物构建了酿酒葡萄分子身份证编码,可区分所有试材。同时,选择多态性高,重复性好的VrZAG79、VrZAG62、VVMD27、VVMD5和 VVS2引物构建了酿酒葡萄品种的SSR位点荧光图谱,这为鉴定和检测葡萄品种及葡萄酒真实性和纯度提供了参考依据。

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	马文瑞
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	王雪薇
	武运
	薛洁

关键词: 酿酒葡萄品种简单序列重复 (simple sequence repeats, SSR) 遗传多样性分析分子身份证编码荧光图谱

Abstract: 10 kinds of Vitis vinifera were used as raw material to analyze genetic relationship and diversity of Various types of vitis vinifera resources by simple sequence repeats (SSR) molecular marker technique. Twelve pairs of polymorphic primers were used for SSR amplification. 213 DNA fragments were generated and all of them were polymorphic bands. The genetic similarity coefficient of SSR amplification results were analyzed by NTSYSpc2.10e software and the genetic similarity coefficient was 0.71 to 0.85 with the average genetic similarity coefficient of 0.76. The clustering dendrogram constructed by unweighted pair-group method with arithmetic mean (UPGMA) method indicated that 10 Vitis materials could be divided into two major groups at the similarity coefficient of 0.73. This study showed that SSR molecular marker analyzed the genetic diversity of different kinds of Vitis vinifera from the molecular level and revealed the relationship between different materials. After alleles assignment,there were only 6 pairs of primers selected out for the construction of germplasms molecular ID. At the same time, high polymorphism, good repeatability of VrZAG79, VrZAG62, VVMD27, VVMD5 and VVS2 primers were selected to establish SSR sites of fluorescence spectra of different varieties and thus construct SSR fingerprint database of Vitis vinifera, which provided a reference for the detection and identification of authenticity and purity of grape varieties and wine.

Key words: Vitis vinifera simple sequence repeats (SSR) genetic diversity molecular ID fluorescence fingerprint

收稿日期: 2018-01-17 出版日期: 2018-09-25 发布日期: 2018-10-30 期的出版日期: 2018-09-25

基金资助: 新疆维吾尔自治区重大科技专项(2017A01001-2);河北省科技计划项目(16222901D)

作者简介: 硕士研究生(武运教授和薛洁教授共为通讯作者,E-mail:764630324@qq.com,825728388@qq.com)。

引用本文:

马文瑞,严密,刘业伟,等. 酿酒葡萄品种遗传多样性的简单序列重复分析和分子身份证的建立[J]. 食品与发酵工业, 2018, 44(9): 232-238.
MA Wen-rui,YAN Mi,LIU Ye-wei,et al. Analysis of genetic relationship of Vitis vinifera resources by SSR markers[J]. Food and Fermentation Industries, 2018, 44(9): 232-238.

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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.016844 或 http://sf1970.cnif.cn/CN/Y2018/V44/I9/232

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[1]	马文瑞 , 邹弯 , 魏玉洁 , 等. 酿酒葡萄品种SSR-PCR体系的优化与建立[J]. 食品与发酵工业, 2018, 44(3): 52-.

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