Abstract: The effects of three L. plantarum strains from different sources on intestinal microflora in rats with metabolic syndrome induced by high-fat-high-sucrose(HFHS)diet were evaluated. Three L. plantarum strains from different sources were individually administered to rats fed a HFHS diet for 12 weeks. Using commercial probiotic Lactobacillus rhamnose GG as a strain control, faecal samples were collected at the end of the experiment. The microbial diversity and composition were measured by Illumina Miseq Sequencing. L. plantarum CCFM591 significantly increased the abundance and microbial diversity of intestinal microbiota by α diversity analysis. PCoA analysis showed that HFHS diet changed the overall structure of microbial community. L. plantarum supplementation displayed various effects on the recovery of gut microbiota dysbiosis. Metagenomic analysis showed that CCFM591 dramatically decreased the ratio of Firmicutes/Bacteroidetes and Proteobacteria/Bacteroidetes induced by HFHS diet. Supplementation with L. plantarum increased the relative abundance of Lactabacillus, and decreased the abundance of Blautia,Coprococcus,Roseburia and [Ruminococcus] at genus level. L. plantarum CCFM591 showed a strong capacity in regulating the gut microbiota dysbiosis induced by HFHS diet.
朱广素,王刚,王园园,等. 三株植物乳杆菌对代谢综合征大鼠肠道菌群的影响[J]. 食品与发酵工业, 2018, 44(9): 53-60.
ZHU Guang-su,WANG Gang,WANG Yuan-yuan,et al. Effects of three Lactobacillus plantarum strains on gut microbiota in metabolic syndrome rats[J]. Food and Fermentation Industries, 2018, 44(9): 53-60.
 WANG Y, BEYDOUN M A, LIANG L, et al. Will all americans become overweight or obese? Estimating the progression and cost of the US obesity epidemic[J]. Obesity, 2008, 16(10):2 323-2 330.  ECKEL R H, ALBERTI K G M M, GRUNDY S M, et al. The metabolic syndrome[J]. Lancet, 2015, 365(9 468):1 415-1 428.  LEY R. Microbial ecology: human gut microbes associated with obesity[J]. Nature, 2006, 444(7 122):1 022-1 023.  ZMORA N, BASHIARDES S, LEVY M, et al. The role of the immune system in metabolic health and disease[J]. Cell Metabolism, 2017, 25(3):506-521.  QIN J, LI Y, CAI Z, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2013, 490(7418):55-60.  HAND T W, VUJKOVIC-CVIJIN I, RIDAURA V K, et al. Linking the microbiota, chronic disease, and the immune system[J]. Trends in Endocrinology & Metabolism Tem, 2016, 27(12):831-843.  MACFARLANE G T, MACFARLANE S. Models for intestinal fermentation: association between food components, delivery systems, bioavailability and functional interactions in the gut[J]. Current Opinion in Biotechnology, 2007, 18(2):156-162.  VIJAYKUMAR M, AITKEN J D, CARVALHO F A, et al. Metabolic syndrome and altered gut microbiota in mice lacking toll-like receptor 5[J]. Science, 2010, 328(5 975):228-231.  GAFFEN S L, JAIN R, GARG A V, et al. The IL-23-IL-17 immune axis: from mechanisms to therapeutic testing[J]. Nature Reviews Immunology, 2014, 14(9):585-600.  DUPONT A W, DUPONT H L. The intestinal microbiota and chronic disorders of the gut[J]. Nature Reviews Gastroenterology & Hepatology, 2011, 8(9):523-531.  BROWN J M, HAZEN S L. Microbial modulation of cardiovascular disease[J]. Nature Reviews Microbiology, 2018,16(3):171-181.  DAVID L A, MAURICE C F, CARMODY R N, et al. Diet rapidly and reproducibly alters the human gut microbiome[J]. Nature, 2014, 505(7 484):559-563.  GIBSON G R, PROBERT H M, LOO J V, et al. Dietary modulation of the human colonic microbiota: updating the concept of prebiotics[J]. Nutrition Research Reviews, 2004,17(2):259-275.  MACCALLUM I, PRZYBYLSKI D, GNERRE S, et al. ALLPATHS 2: small genomes assembled accurately and with high continuity from short paired reads[J]. Genome Biology, 2009, 10(10):R103.  LOMAN N J, CONSTANTINIDOU C, CHAN J Z, et al. High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity[J]. Nature Reviews Microbiology, 2012, 10(9):599-606.  SABIROVA J S, XAVIER B B, COPPENS J, et al. Whole-genome typing and characterization of blaVIM19-harbouring ST383 Klebsiella pneumoniae by PFGE, whole-genome mapping and WGS[J]. Journal of Antimicrobial Chemotherapy, 2016, 71(6):1 501-1 509.  TYAGI A K, SAHDEO P. Commentary: Probiotic and technological properties of Lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis[J]. Frontiers in Microbiology, 2015, 5:766.  MAO B, LI D, ZHAO J, et al. Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice[J]. Journal of Agricultural & Food Chemistry, 2015, 63(3):856-863.  毛丙永. 功能性低聚糖对肠道细菌的影响及机制[D]. 无锡:江南大学, 2015.  BI Y, LI C, LIU L, et al. IL-17A-dependent gut microbiota is essential for regulating diet-induced disorders in mice[J]. Science Bulletin, 2017, 62(15):1 052-1 063.  WANG J, TANG H, ZHANG C, et al. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice[J]. Isme Journal, 2015, 9(1):1-15.  KIM S W, PARK K Y, KIM B, et al. Lactobacillus rhamnosus GG improves insulin sensitivity and reduces adiposity in high-fat diet-fed mice through enhancement of adiponectin production[J]. Biochemical & Biophysical Research Communications, 2013, 431(2):258-263.  WEST N P, PYNE D B, CRIPPS A, et al. Gut Balance, a synbiotic supplement, increases fecal Lactobacillus paracasei but has little effect on immunity in healthy physically active individuals[J]. Gut Microbes, 2012, 3(3):221-227.  CANI P D, BIBILONI R, KNAUF C, et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice[J]. Diabetes, 2008, 57(6):1 470-1 481.  TURNBAUGH P J, LEY R E, MAHOWALD M A, et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature, 2006, 444(7 122):1 027-1 231.  LIM S M, KIM D H. Bifidobacterium adolescentis IM38 ameliorates high-fat diet-induced colitis in mice by inhibiting NF-κB activation and lipopolysaccharide production by gut microbiota[J]. Nutrition Research, 2017, 41:86-96.  BOSSHARD P P, ZBINDEN R M. Turicibacter sanguinis gen. nov. sp nov. a novel anaerobic, gram-positive bacterium[J]. International Journal of Systematic & Evolutionary Microbiology, 2002, 52(4):1 263-1 266.  PRESLEY L L, WEI B, BRAUN J, et al. Bacteria associated with immunoregulatory cells in mice[J]. Applied & Environmental Microbiology, 2010, 76(3):936-941.  DUNCAN S H, BELENGUER A, HOLTROP G, et al. Reduced dietary intake of carbohydrates by obese subjects results in decreased concentrations of butyrate and butyrate-producing bacteria in feces[J]. Applied & Environmental Microbiology, 2007, 73(4):1 073-1 078.  JAKOBSDOTTIR G, XU J, MOLIN G, et al. High-fat Diet reduces the formation of butyrate, but increases succinate, inflammation, liver fat and cholesterol in rats, while dietary fibre counteracts these effects[J]. Plos One, 2013, 8(11):e80476.