Abstract: Echinocandin B (ECB) is the key precursor of anidufungin, mainly synthesized by microbial fermentation. In this experiment, the scale-up conditions of Aspergillus nidulans ZJB09223 for ECB fermentation was optimized in the fermentor. The effects of different types of agitating paddles, stirring speed and aeration volume on the Echinocandin B fermentation were investigated to achieve the basic conditions of industrialization. The comparison of different impeller types showed that flat-straight leaf disc tubine type agitator was more suitable for ECB fermentation process. The optimum aeration amount was 1.7 VVM, and the stirring speed was 600 r/min in the first 6 days and 400 r/min in the next 9 days to obtain the best results of 608 mg/L, which was 193% higher than that under the original conditions.
胡晓龙,秦海彬,吴旭萍,等. 5 L发酵罐中棘白菌素B发酵工艺参数优化[J]. 食品与发酵工业, 2018, 44(9): 130-135.
HU Xiao-long,QIN Hai-bin,WU Xu-ping,et al. Optimization of the fermentation parameters for Echinocandin B production in 5 L bioreactor[J]. Food and Fermentation Industries, 2018, 44(9): 130-135.
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